EMAGE mouse gene expression spatial database

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EMAGE:8844

Crispld1 cysteine-rich secretory protein LCCL domain containing 1 ( MGI:1934666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010145_01	euxassay_010145_02	euxassay_010145_03	euxassay_010145_04

euxassay_010145_05	euxassay_010145_06	euxassay_010145_07	euxassay_010145_08	euxassay_010145_09

euxassay_010145_10	euxassay_010145_11	euxassay_010145_12	euxassay_010145_13	euxassay_010145_14

euxassay_010145_15	euxassay_010145_16	euxassay_010145_17	euxassay_010145_18	euxassay_010145_19

euxassay_010145_20	euxassay_010145_21	euxassay_010145_22	euxassay_010145_23	euxassay_010145_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8844_wholemount_strong.wlz

8844_wholemount_moderate.wlz

8844_wholemount_weak.wlz

8844_wholemount_possible.wlz

8844_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8844_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 15 16 17 21 22 23 24 weak expression: see section 08 18 19 20

humerus

moderate

regional

moderate expression: see section 01 02 03 04

forelimb digit 1 phalanx

moderate

regional

moderate expression: see section 02 03

forelimb digit 2 phalanx

moderate

regional

moderate expression: see section 01 02 03

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 01

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 05 06

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 05 06 weak expression: see section 17 18 19 20

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 05 06 weak expression: see section 17 18 19 20

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 05 06 weak expression: see section 17 18 19

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 05 06 weak expression: see section 17 18 19

tarsus

moderate

regional

moderate expression: see section 03

fibula

moderate

regional

moderate expression: see section 01 02 22 23 24 weak expression: see section 21

tibia

moderate

regional

moderate expression: see section 01 02 22 23 24 weak expression: see section 21

femur

moderate

regional

moderate expression: see section 01 02 03 04 22 23 24 weak expression: see section 17 18 19 20 21

diencephalon meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord meninges

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

nasal cavity

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17 18 moderate expression: see section 12

mandible

moderate

regional

moderate expression: see section 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16

maxilla

moderate

regional

moderate expression: see section 17 18 19 20 weak expression: see section 07 09 10 13 16

thyroid cartilage

strong

regional

strong expression: see section 12 13 14 15 16

trachea cartilaginous ring

strong

regional

strong expression: see section 14 15

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

basioccipital bone

moderate

regional

moderate expression: see section 01 02 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

basisphenoid bone

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

temporal bone petrous part

moderate

regional

moderate expression: see section 02 23 24

vault of skull

moderate

regional

moderate expression: see section 01 02 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

clavicle

moderate

regional

moderate expression: see section 09 10 11 12 18 19 20

sternum

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32049
Entity Detected:	Crispld1, cysteine-rich secretory protein LCCL domain containing 1 ( MGI:1934666)
Sequence:	sense strand is shown >T32049 ATGGGATGTGGAGCTGGAAAGATCTGCAGAATCCTGGGCTGAAATGTGTTTGTGGGAACATGGCCCCGCG AGCCTGCTGCCCTCCATTGGACAGAATTTGGGGGCACACTGGGGAAGATACCGACCCCCAACCTTTCATG TACAAGCATGGTATGATGAAGTGAGAGACTTTAGCTACCCATATGAAAATGAGTGTGACCCTTACTGTCC TTTCAGATGTTCTGGCCCTGTTTGTACACACTACACTCAGGTGGTGTGGGCTACTAGTAGCAGAATAGGC TGTGCCGTCAATCTGTGCCACAACATGAACATCTGGGGGCAGATATGGCCCAAGGCAGTCTACTTGGTGT GCAATTACTCCCCCAAGGGAAACTGGTGGGGCCACGCACCTTACAAACATGGAAGGCCTTGTTCTGCTTG CCCACCCAGTTTTGGAGGAGGCTGTAGAGAAAACTTGTGCTACAAAGAAGGGTCAGACAGGTATTATACC CCTCGAGAAGAAGAAACAAATGAAATTGAACGGCAGCAGTCTCAGGTCCATGACACCCATGTTCGAACAA GATCAGATGACAGTGACAGAAATGACGTCATCAGCACTCAGCAGATGTCTCAGATTGTTTCTTGTGAAGT AAGATTGAGAGACCAGTGTAAAGGGACCACCTGCAACAGGTATGAGTGCCCTGCAGGCTGCTTGGACAGT AAAGCTAAAGTAATTGGGAGTGTACATTATGAAATGCAATCCAGTATCTGCCGAGCTGCCATCCACTATG GGATAATAGACAATGAAGGTGGGTGGGTAGATGTCACTCGACAAGGGAGAAAGCATTACTTCATCAAATC CAACAGAAATGGCATTCAAACAATTGGAAAATATCATTCTGCTAATTCCTTCACAGTCTCCAAAGTTACA GTTCAAGCTGTGACCTGTGAAACTACAGTGGAACAGCTCTGTCCATTCCATAAGCCTGCTTCTCATTGCC CCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4458200), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57847. Forward Primer - name:057847_F_IRAV88_d12_Cocoacrisp, sequence:ATGGGATGTGGAGCTGGA; Reverse Primer - name:057847_R_SP6_IRAV88_d12_Cocoacrisp, sequence:TCTGGGGCAATGAGAAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8845 same embryo
	EMAGE:8843 same embryo
	EMAGE:8847 same embryo
	EMAGE:8846 same embryo
	EMAGE:8848 same embryo
	EurExpress:euxassay_010145 same experiment
	MGI:4824057 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source