EMAGE mouse gene expression spatial database

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EMAGE:10711

Pde8b phosphodiesterase 8B ( MGI:2443999)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003225. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003225_01	euxassay_003225_02	euxassay_003225_03	euxassay_003225_04

euxassay_003225_05	euxassay_003225_06	euxassay_003225_07	euxassay_003225_08	euxassay_003225_09

euxassay_003225_10	euxassay_003225_11	euxassay_003225_12	euxassay_003225_13	euxassay_003225_14

euxassay_003225_15	euxassay_003225_16	euxassay_003225_17	euxassay_003225_18	euxassay_003225_19

euxassay_003225_20	euxassay_003225_21	euxassay_003225_22	euxassay_003225_23	euxassay_003225_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10711_wholemount_strong.wlz

10711_wholemount_moderate.wlz

10711_wholemount_weak.wlz

10711_wholemount_possible.wlz

10711_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10711_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 04 05 23 24

hindlimb digit 1 dermis

moderate

regional

moderate expression: see section 23

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 23 24

hindlimb digit 2 skin

moderate

regional

moderate expression: see section 06 07

hindlimb digit 2 dermis

moderate

regional

moderate expression: see section 22 23

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 04 05 23 24

hindlimb digit 3 skin

moderate

regional

moderate expression: see section 06 21

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 04 06 22 23 24

hindlimb digit 4 skin

moderate

regional

moderate expression: see section 21

hindlimb digit 4 dermis

moderate

regional

moderate expression: see section 22

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 05 06 22 23 24

hindlimb digit 5 dermis

moderate

regional

moderate expression: see section 22

adrenal gland

weak

regional

weak expression: see section 17 18 19

pancreas

moderate

regional

moderate expression: see section 09 16 17 18 19 weak expression: see section 10 11 12 13 14

dorsal grey horn

moderate

regional

moderate expression: see section 14

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

anterior naris

moderate

regional

moderate expression: see section 12 13 14 15 16 17

posterior naris

moderate

regional

moderate expression: see section 12 13 14 15 16 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

kidney calyx

strong

regional

strong expression: see section 17 18 21 22 moderate expression: see section 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5413
Entity Detected:	Pde8b, phosphodiesterase 8B ( MGI:2443999)
Sequence:	sense strand is shown >T5413 CCACGCGTCCGGTCCCTTGGAGCGACCAGACCCTTCTTGCAGAGAGAGCGAGGTTAATGGTGCAAAGGTA CAGCTGTCTAGTGCGCTCCATGAGCGGCTGCTGCTAGGGGCACAGGCACCTGCACGTTGGGACAGCAGCT GGGGATCGCAGCGGGTATGGACCAGCAGCAGAGCGTGCGCCAGGTGTCTTCTGCAGAGGTGCGCATTGGG CCCATGAGACTGACACAGGACCCTATCCAGGTTCTGCTAATCTTCGCAAAGGAAGATAGCCAGAGTGATG GCTTCTGGTGGGCCTGTGACAGAGCTGGCTACAGATGCAACATCGCTCGCACTCCAGAGTCGGCCCTCGA GTGCTTTCTGGACAAGCATCATGAAATTATTGTGATCGATCACAGGCAGAGCCGGAACTTTGATGCAGAA GCCGTGTGCAGGTCAATCCGGGCCACGAACCCCTCTGAGCACACGGTGATCCTGGCTGTCGTTTCACAAG CATCTGATGACCATGAAGAGGCCTCAGTCCTTCCTCTTCTTCATGCAGGCTTCAACCGGAGATTTATGGA GAACAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACACGGGGAAGTTCGCTCACAGTTC AAATTACGGGCCTGTAATTCAGTGTTTACAGCGTTAGACCACTGTCACGAAGCCATAGAAATAACAAGTG ATGACCATGTGATTCAGTATGTCAACCCTGCCTTTGAGAGGATGATGGGCTACCACAAAGGGGAGCTCCT GGGGAAGGAACTCGCTGACCTACCTAAGAGTGACAAGAACCGGGCAGACCTCCTGGACACCATCAACACG TGCATCAAGAAGGGAAAGATTCACCGGATTCATCGTGACTCCGGGGACAACTCTCAGACAGAGCCTCATT CGTTCAGACACAAGAGCAGGAGGAAAGAGTCCATTGATGTGAAATCAATATCTTCTCGGGGCAGTGATGC GCCAAGCCTACAGAATCGCCGCTACCCCTCCATGGCCAGGATCCACTCCATGACCATTGAGGCTCCCATC ACAAAGGTTATAAATATAATCAATGCAGCTCAGGAGAACAGCCCAGTTACAGTAGCAGAGGCCTTGGACA GAGTCCTAGAAATTTTACGGACCACAGAACTGTATTCCCCTCAGCTGGGAACCAAAGATGAGGATCCCCA CACCAGTGACCTTGTTGGGGGCCTGATGACTGATGGCTTGAGGAGACTGTCAGGGAACGAGTATGTGTTT ACAAAGAATGTGCACCACAGTCACAGTCACCTCTCGATGCCCATCACCATCAATGATGTCCCCCCAAGTA TCGCCCAGCTCCTTGATAATGAGGAAAGCTGGGACTTCAACATCTTTGAATTGGAAGCCGTTACACATAA AAGGCCACTTGTGTACCTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGTGAATTTTTAAACTGTACT GAAACCACTCTTCGGGCCTGGCTGCAAGTGATTGAAGCCAACTACCACTCCTCCAACGCCTACCACAACT CCACCCATGCTGCCGATGTACTGCATGCCACGGCTTTCTTCCTTGGAAAGGAAAGAGTGAAGGGAAGCCT GGACCAGCTGGATGAGGTGGCTGCTCTGATTGCTGCCACAGTCCACGACGTGGATCACCCCGGAAGGACC AACTCTTTCCTGTGCAACGCCGGCAGCGAGCTCGCTGTGCTCTACAATGACACTGCAGTCTTGGAGAGTC ACCACACAGCCCTGGTCTTCCAGCTCACAGTCAAGGACACCAAGTGCAACATTTTCAAGAATATTGACAG GAACCACTATCGAACGCTGCGCCAGGCTATTATCGACATGGTTTTGGCTACAGAGATGACAAAACACTTC GAGCACGTGAACAAATTCGTGAACAGCATCAACAAGCCACTGGCAGCTGAGAGTGAGGGCAGTGATTGTG AATGCAACCCTACCGGGAAGAACTTTCCTGAGAACCAAATCCTCATCAAACGCATGATGATTAAGTGTGC CGACGTGGCCAACCCGTGCCGGCCCTTGGACCTGTGCATTGAATGGGCTGGAAGGATCTCCGAGGAGTAC TTTGCTCAGACTGATGAAGAGAAGAGGCAGGGGCTGCCTGTGGTGATGCCAGTGTTCGACCGCAATACCT GCAGCATCCCCAAGTCCCAGATCTCCTTCATCGACTACTTCATAACAGACATGTTTGATGCTTGGGATGC CTTCGCTCACCTGCCTGCCCTGATGCAGCACCTGGCCGATAACTATAAACACTGGAAGACACTGGATGAC CTCAAGTGCAAAACTCTGAGACTTCCATCTGACAGCTGAAGCCACGCCCGAGAAGGGGACACTGTGAATC ACAGGGTAGAACAAGCAAGAGGGTTTCCTTTAAAATGGAAATGACAGGCGGACACTAAGGGGATGGTGTT TAATTTCCGACCTGAATCATGCACGCCCCCAAACTTCATTTCCAGAAAGTTATATTCCATCAATAAAAAT ATGTTCCTTTGAATCACTCAGTGACAAAACGAGCTGCCTGGCAGCTTCTTCTGCCAAGCTGTTAGCCTGC GTGCCCTTGTAAAGCCGGAGCTGACTGACTTACTGACGGTACAAGCAGGACACGAGAAGCAAAGGCCTGT TGTGTGTCCATCCTCCTGACGGAGGTCAGGTAGCTTAGCCAGAGTTCAGCTCAACTGTTACCAGAAAACT AACGACGGAGGCTTTCCTCAGTGGCAAAAGGATGAAGGATTCCTAAACATCTTCTCTGAGCAAATGGATG TGAGGAAGGAGCTCATTAAAATGAGCAGAATGAGAATGAGATGGTCAGTAAACTAATTAGTTAGCACTGT GGTTCCCATGGCTACATGGGAGACAGTGTGATATCCAAGTCAGAACACATCCTGGGCCTGCCGGGTGTCC CGTGGATGGGTGCCCGTCACCCGTGGAGCCCCCTTTCCTCCGTGGAACCCGCCTTTTGGAAGCCAGCTGA TAGGGAAGGAGTAGGATCTCAGTTAGGGGAGTCCTTGAGCACCCCTTACTTCAGGCTCAAGGACACAGCC ACAAGTCAGAGTATCTATAAAGCCATTAAGTCTGAATGCTCGTGGGTAAGGTTTTTTTTAAAAAAAAAAA ATTAAGAGAAGATAAATTTATATATATTTGCTGTTCAAAGATTTTTATTAAAATAATCTAAATGTCCCGA ATCTTCACCCCTAGTTTGCTGGGCATTACCTGTCTGTCTATATCTGGGGAGGAAATGGGGAAGTTCCAAG GTTCGGATATCCTTAATTTATATTTGAGATCATGCCATTTGCTTTAATGATGTAAAAAGAGACTGCAAGA AGTGTAAATATGTCGGGCGGGACAAAGGCATGGAGAGGCAGTAAATACTATTTTATGCTACCGCCCGTAC GCTGACATTCCTGAAGCACAAGGGCATATTTTTATACAACTTTTCACAAGGCTCTGTGCTCTGCATACGG TCAGACAGATCTCCCCTCTCTCTTGTCAGCCGTCCCGCCTCTATGTGTTACGAACATCAGAAACCAAGTA AACAAAATACCTTACTTTTATTTTATCTGTACTCCACGATGCATTTTAAAGATGTATAGAAACTGAACTG ATAGAATCAGTAGCTACATTTTTTTTTCCAAGTGAACATTGGACCTTGGAGTATGTGGAAATTGGTACAT GGGGAAGAAAGAGACAACTTATCGCTACGTAATGTGCCAATGTTTTTTTTTTCTATGTTTTGTACCAAAG TGTAAACATATATGACAATTCCATGCAAAGAAATGCATCTAAATTATTTTTGTTAGATGGTGAGAGGGCT TGCTTGCTCAGGACAGGAGATGGCTTTCTATTGTACTGCTGCGTTATCTACATGTGATGAAATGTTTTTG TGAAGTACTTGTAACTAAATTCTTACAGCCATTTTTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5367055 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5367055 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10710 same embryo
	EMAGE:10709 same embryo
	EMAGE:10712 same embryo
	EMAGE:10714 same embryo
	EMAGE:10713 same embryo
	EurExpress:euxassay_003225 same experiment
	MGI:4827123 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source