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EMAGE:11378

Ndufaf3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 ( MGI:1913956)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378
"Pseudo-wholemount" of euxassay_006731. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006731_01 euxassay_006731_02 euxassay_006731_03 euxassay_006731_04
EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378
euxassay_006731_05 euxassay_006731_06 euxassay_006731_07 euxassay_006731_08 euxassay_006731_09
EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378
euxassay_006731_10 euxassay_006731_11 euxassay_006731_12 euxassay_006731_13 euxassay_006731_14
EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378 EMAGE:11378
euxassay_006731_15 euxassay_006731_16 euxassay_006731_17 euxassay_006731_18 euxassay_006731_19
EMAGE:11378 EMAGE:11378 EMAGE:11378
euxassay_006731_20 euxassay_006731_21 euxassay_006731_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11378Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11378_wholemount_strong.wlz
11378_wholemount_moderate.wlz
11378_wholemount_weak.wlz
11378_wholemount_possible.wlz
11378_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11378_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 16 17 moderate expression: see section 15 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8171
Entity Detected:Ndufaf3, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 ( MGI:1913956)
Sequence:sense strand is shown

>T8171
CGATTTCGAGACTAGCTAGGGAAATTTTAGCCATCTGCCCCGCCCCCGGAGCCCGATTCTCCAAAGCCTG
GGATTAAGGGCCTGGAGGCTGGCACCACACCCTGGCCAGGTGTTCATAACAGGACCAGGTGCGCAGCTGC
CCCGCCCTGGTCCCTGGGAGCCCGAATAATGGCTGAGAATCCCAGGAACAGGCCGCGGCAGTGGCCTAAA
CAATACCCTTAGGGCGCCCGTACTAGCAGGTGTTTTTCCCCCTCCTCCTTAGGGACTGTCGCTGGAGGCA
TGGGGCGGGGTGGGCGACAGGTGCTGACACACCCGCATGGGGAGACAGAGGCTCTGCATTGGCCTACTGC
GCCGTTCAAGGTGCGCGGTGCCCTCCGGGACGCAAGGGGCGCTGTGGCTACTTTGAGACAGACAACTCTG
TCCCTACGACCGGGTCGTTAAGCGCCGTTGCAGCCATGGCTACTGCTCTAGGATTTCGCTGCCTTTTCCG
AACGCGACCTGCGCCGCTCTGTCGGCACGTCGACCGGCTGTGGAGGAACCCGCGTCGGGGACACCGGCTT
TCTCCGGCGGACGATGAGTTATACCAGCGGACTCGCATCTCACTGCTGCAAAGCGAGTTCCCTCAGGCCG
TGTACATCGACAGCTACAGTAGCAGAGGCTTCACTATCTGCGGAAATCGCGTGTTTGGCCCTTGCGTGCT
GCTCCCACAGACGGTGGTCCATTGGAACGTGGGATCTCATCAGGATATCACTGAAGAGAGCTTCTCCCTT
TTCTGGATGCTGGAACCTAGGATAGAGATTGTTGTGGTGGGCACTGGAAACAAGACTGAGCGGCTGCACC
CTCAAGTACTACAGGCCATGAGGCAGCGGGGAATTGCTGTGGAGGTCCAGGACACGCCCAATGCCTGTGC
CACTTTCAACTTCCTGTGCCACGAAGGTCGAGTGACAGGAGCTGCCCTCATTCCCCCTCCTGGAGAGACT
GCACTGGCTTCTTTGGGTCAAGCCTCAGAATGACACATCAGGAACCATCAAGGAGGGCACTGCATGCTCT
CAACGCTTTCTCCACACTTGTCATGCTCACCCAGGATAATAATTTATACTCCTCTTAAAAAAAAAAAAAA
AAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5118121 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5118121 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11377 same embryo
 EMAGE:11376 same embryo
 EMAGE:11375 same embryo
 EMAGE:11379 same embryo
 EurExpress:euxassay_006731 same experiment
 MGI:4826643 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS