EMAGE mouse gene expression spatial database

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EMAGE:11575

Tmem132a transmembrane protein 132A ( MGI:2147810)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003650. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003650_01	euxassay_003650_02	euxassay_003650_03	euxassay_003650_04

euxassay_003650_05	euxassay_003650_06	euxassay_003650_07	euxassay_003650_08	euxassay_003650_09

euxassay_003650_10	euxassay_003650_11	euxassay_003650_12	euxassay_003650_13	euxassay_003650_14

euxassay_003650_15	euxassay_003650_16	euxassay_003650_17	euxassay_003650_18	euxassay_003650_19

euxassay_003650_20	euxassay_003650_21	euxassay_003650_22	euxassay_003650_23	euxassay_003650_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11575_wholemount_strong.wlz

11575_wholemount_moderate.wlz

11575_wholemount_weak.wlz

11575_wholemount_possible.wlz

11575_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11575_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 weak expression: see section 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 19 20 21 22 23 weak expression: see section 18

vagus x ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 19 weak expression: see section 18

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 16 17 18

esophagus

strong

regional

strong expression: see section 13

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08

lower jaw molar

moderate

regional

moderate expression: see section 06 19 20

upper jaw molar

moderate

regional

moderate expression: see section 06 19 20

left lung lobar bronchus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6635
Entity Detected:	Tmem132a, transmembrane protein 132A ( MGI:2147810)
Sequence:	sense strand is shown >T6635 GCCACAGCGGCTGGCGCGGAGCGGGCGCGGGAGATGTGGGGACAGATTGCAGCCACCTGAGCCGCGCGCC GGTTACCCTCCTTTGGTGGGAAGGAGTGCGCCTCGATGACTGAACGCAAGGCTGCGGCCCCTCGGGGGCC CTACGGTGCCTGGTTCTGCCTCCTGGTGGCTCTCGCCCTGGAAGTCGTGAGAGTTAGCAGTAACCATGAT ACCCTGGACCCCATCTACCTGCCAGTGGCCCTGGAACTCCTAGATGCCCCTGAGCATTTTCGTGTGCAGC AGGTGGGCCATTACCCACCTGCCAACTCTTCCCTGGCCTCCCGCTCTGAGACTTTCCTGCTCATGCAGCC CTGGCCCAGGGCACAGCCGCTTCTCCGGGCCTCCTACCCACCCTTTGCCACTCAGCAGGTGGTCCCTCCT AGGGTCACCGAGCCCCACCGGCGGCCTGTCCCTTGGGATGTGCGGGCTGTTTCCGTGGAAGCAGCGGTGA CTCCAGCAGAACCCTATGCCCGTGTCCTCTTCCACCTCAAAGGGCAGGATTGGCCACCAGGACCTGGCAG CTTGCCCTGTGCCCGACTACATGCCACACACCCTGCAGGCACTGCCCACCGAGCCTGCCGCTTCCAGCCA TCTCTGGGTGCCTGTGTGGTGGAACTGCAATTCCCCTCTCACTGGTTCTCCCAAAGTGCCACCACAAGGG CCGAACTGGCCTACACGCTGGAGCCTGCAGGCGAGGGCCCTGGGGGCTGTGGCCTTGGCACAGAGGAGGA ACCCAGGGAGCAGGCTCTCCCAGTGGGTGGTGTGGAGCTGCACCCTGAGGACCCCCCACAGTACCAGGAG GTACCTCTGGATGAAGCAGTCACTTTGCGTGCACCTGATGTGCCAATGAGGCCTGGCCAGCTCTTCACTG CCACCCTTCTGCTTCGACACAACTTCACTGCGAGCCTTCTTACTCTGCGGATTAAGGTGAAGAAAGGGCT GCAGGTGATAGCTGCCCGCCCAGCCCAGCCCACCCTCTGGACTGCTAAGTTAGACCGCTTCAAGGGCTCA AAGCACCACACCAGCCTGATCACCTGTCACCGTGCTGGGCCTGCAGGGCCAGATTCCAGCCCCCTTGAAC TGTCCGAGTTCCTGTGGGTGGACTTTGCAGTGGAGAACAGCACTGGTGGGGGTGTGGCAGTCACTCGCCC TGTCACGTGGCAGCTGGAGTACCCAGGTCAGGCCCCCGAAGCAGAGAAGGACAAAATGGTGTGGGAGATC CTGGTGTCTGAGCGGGACATCAGAGCCCTCATCCCTCTGGCCAAGGCTGAGGAGCTGGTGAACACGGCTC CATTGACTGGAGTGCCCCAGCGTATTCCGGTGCGGCTCGTCACTGTGGACAGTGGGGGAGCCTTGGAGGA GGTGACAGAGCACATTGGCTGTGAGTCAGCCAATACACAGGTCCTGCAGGTATCTGAGGCCTGTGATGCT GTGTTCGTGGCTGGCCAGGAGAGCCGGGGTGCCAAGGGGGTGAGGGTGGACTTCTGGTGGCGCCGACTTC GGGCCTCACTAAAGCTGACTGTATGGGCCCCACTCCTGCCCTTGCGCATTGAGCTGACTGACACCACTCT GGAACAAATCCGAGGCTGGAGGGTCCCAGGCTCAGCAGAAGGGCAGCTGGAGCCTGAGACTGCTGCTGAG GAGGTGGAGCGGCGCTCCCGTGGCTGCCGCCTGCAGTACCAGAGAGCTGGTGTGCGCTTCTTGGTGCCCT TTGCGGCTCACCCGCTGGACGGTGGCCGTCGCCTCACCCACCTACTCGGTCCTGACTGGTTGCTGGATGT GAGTCACCTCGTGGCAGCCCATGCCCATGTGCAAGATCCTCGCATAGCCTCCTTGGAAGGTGGCCGCATC CTAGTGGGACGGGAGCCTGGAGTCACCTCTATCGAGGTTCGTTCCCCGCTGTCTGACGCCATCCTGGGGG AGCAGGCACTGGCTGTGACAGACGACAAGGTCTCAGTGCTGGATCTGCGAGTACAGCCGGTGATGGGCAT CTCACTGTCCTTGAGCCGGGGCATGTCCCACCCTGGAGAAGTCACAGCCACATGCTGGGCCCAGTCAGCT CTTCCAGCCCCAAAGCAGGAGGTGGCCCTCTCCCTGTGGCTGTCCTTCTCTGACCACACTTTGGCCCCAG CCGAGCTCTACGACCGCAATGACCTGGGGCTGTCGGTCTCAGCTGAGGAGCCCAGTGCTGTTCTGCCAGC CGAAGAGCAGGGAGCCCAGCTTGGTGTGGTGGTGAGTGGTGTGGGTGCCGAAGGGCTGCCCCTACACGTG GCTCTGCACCCACCCGAACCCTGCCGCCGGGGCCGCCATCGCGTTCCCCTGGCATCAGGCACCGCCTGGC TGGGGTTGCCCCCTTTGCCCACACCAGTTCCTGCCCTCCCATCCAGCCCTGTCCGGACCTCACCGTTCAC AGAAGCCACGGTGGAGGGAAAGCGGCAGATAGCAGGTGATATGGGGGGCCATGTGGGTATTAGGGGCAAG TTTGAGCGGGCAGAGGAAGAAGCTGGGAAGGAAGAAAATGAGGCCAAAGAAGAGGAGGAAGATGAGGAAG AGATGGTGCCTGCCCCTCAGCGTGTCACCGACCTAGAGCTGGGGATGTATGCTCTTCTGGGCATCTTCTG TTTGGCCATCCTCATCTTCCTGGTCAATGGTGTGGTCTTTGTGTTGCGCTACCAGCGCAAAGAGCCTCCA GACAGCGCTACAGACCCTGCCTCCCCTCAGCCACATAACTGGGTCTGGCTGGGCACCAATCAGGAGGAAC TGAGTCGGCAGCTGGACCGATGCTCTTCCTCGGGTCCACCCAAGGGGGAGGGGGGCTGCCCTTGTGAAAG TGGGGCAGGAGGGGATGCCTCAACCGTGGCCCCCAGTGCTTCAGAGAGCCCTGCTGGCTCCTCGAGCACC CTGGCCCGCAAAGAAGCCGGGGGACGTCGGAAGCGAGTCGAGTTTGTGACATTTGCACCAGCACCCCCAA CCCAGCCGCCTGAGGAGCCTGTAGGGGCCCCCGCTGTACAGTCCATCCTGGTGGCAGGTGAGGAGGACAT CCGCTGGGTGTGCGAGGACATGGGGCTGAAGGACCCAGAGGAGCTTCGAAACTACATGGAGAGGATCCGG GGCAGCTCCTGACCACTCCCTGACCTGCCAGGGGCAACTTGGCCCACCAGCTCAGGCACTGGGACCAATG GGATCGCTGGCTAATCGTCTTGCCTGACCCTCATTCTCATCCTCTGGTGCTTGTTGATCTGAGTCCCCTG CTGGGTCCCCTCGGGGACCCCGTCCCAGGCCCCTTTCACCCCCACCCCTTGTCATGGACTATGGTCAAGA GGGAGGGCGCATGCCCCTTATTTATGGGAACCACTTCATTCTACTGTTGGGTACAGAATAAATGAGAAAA GAATGGGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599652 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599652 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11573 same embryo
	EMAGE:11572 same embryo
	EMAGE:11574 same embryo
	EMAGE:11577 same embryo
	EMAGE:11576 same embryo
	EurExpress:euxassay_003650 same experiment
	MGI:4828771 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source