Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12272

Macf1 microtubule-actin crosslinking factor 1 ( MGI:108559)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272
"Pseudo-wholemount" of euxassay_011134. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011134_01 euxassay_011134_02 euxassay_011134_03 euxassay_011134_04
EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272
euxassay_011134_05 euxassay_011134_06 euxassay_011134_07 euxassay_011134_08 euxassay_011134_09
EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272
euxassay_011134_10 euxassay_011134_11 euxassay_011134_12 euxassay_011134_13 euxassay_011134_14
EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272
euxassay_011134_15 euxassay_011134_16 euxassay_011134_17 euxassay_011134_18 euxassay_011134_19
EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272 EMAGE:12272
euxassay_011134_20 euxassay_011134_21 euxassay_011134_22 euxassay_011134_23 euxassay_011134_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12272Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12272_wholemount_strong.wlz
12272_wholemount_moderate.wlz
12272_wholemount_weak.wlz
12272_wholemount_possible.wlz
12272_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12272_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
upper arm muscle
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06
upper leg muscle
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 20 21 22 23 24
diaphragm
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
forearm rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
hand
moderate moderate
regionalmoderate expression: see section 01 02 03
foot
moderate moderate
regionalmoderate expression: see section 07 08 23 24
lower leg rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23 24
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 07 21 22 23 24
facial vii ganglion
weak weak
regionalweak expression: see section 05 06
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 20
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 19 20 21 22
vagus x ganglion
weak weak
regionalweak expression: see section 09 19
spinal cord
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18
esophagus
moderate moderate
regionalmoderate expression: see section 16
tongue muscle
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
metanephros
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18 19 20 21
left lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13
right lung
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20 21 22 23 24
tail paraxial mesenchyme
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19
extraembryonic component
moderate moderate
regionalmoderate expression: see section 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36609
Entity Detected:Macf1, microtubule-actin crosslinking factor 1 ( MGI:108559)
Sequence:sense strand is shown

>T36609
GGCTTTCTGAAGCAGAACAAACACTTCGCTTTCGAGGAGCCCTTCCTGATGACACGGAGGCCCTACAATC
TCTCATTGACACCCATAAGGAATTCATGAAGAAAGTAGAAGAAAAGCGGGTAGATGTGAACACAGCAGTG
GCGATGGGAGAGGCCATCCTGGCTGTCTGCCATCCCGACTGCATCACGACCATCAAGCACTGGATCACCA
TCATCAGAGCTCGATTCGAGGAGGTCCTGACGTGGGCAAAGCAGCACCAACAGCGCCTGGAAACTGCTCT
GTCAGAACTAGTGGCTAATGCTGAGCTCCTGGAAGAACTTCTGGCATGGATCCAGTGGGCTGAGACCACC
CTAATCCAGCGGGATCAGGAGCCCATCCCTCAGAACATAGACAGAGTTAAAGCTCTTATCACTGAGCATC
AGTCATTTATGGAGGAGATGACACGCAAACAACCTGACGTGGACAGGGTCACCAAGACATATAAGAGGAA
GAGTGTAGAACCCACCCATGCACCTTTCATGGAGAAATCCCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78187. Forward Primer - name:078187_F_cDNA_Macf1, sequence:GGCTTTCTGAAGCAGAACAAAC; Reverse Primer - name:078187_N_SP6_cDNA_Macf1, sequence:CGGGATTTCTCCATGAAAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12277 same embryo
 EMAGE:12273 same embryo
 EMAGE:12275 same embryo
 EMAGE:12276 same embryo
 EMAGE:12274 same embryo
 EurExpress:euxassay_011134 same experiment
 MGI:4826052 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS