EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12298

Ift172 intraflagellar transport 172 homolog (Chlamydomonas) ( MGI:2682064)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010945. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010945_01	euxassay_010945_02	euxassay_010945_03	euxassay_010945_04

euxassay_010945_05	euxassay_010945_06	euxassay_010945_07	euxassay_010945_08	euxassay_010945_09

euxassay_010945_10	euxassay_010945_11	euxassay_010945_12	euxassay_010945_13	euxassay_010945_14

euxassay_010945_15	euxassay_010945_16	euxassay_010945_17	euxassay_010945_18	euxassay_010945_19

euxassay_010945_20	euxassay_010945_21	euxassay_010945_22	euxassay_010945_23	euxassay_010945_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12298_wholemount_strong.wlz

12298_wholemount_moderate.wlz

12298_wholemount_weak.wlz

12298_wholemount_possible.wlz

12298_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12298_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9396
Entity Detected:	Ift172, intraflagellar transport 172 homolog (Chlamydomonas) ( MGI:2682064)
Sequence:	sense strand is shown >T9396 GTGCGTCAGCGGTTAGCGCCGCCGATGCTTGGCGGGTTGTCCAGGTAACCACGGGAGTTGTCTCGGTCAG TGAGCATCCGAGACCGGTGTACGTCATGCAGTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGA GCTGCGAAGGTGACATGCATGGCCTGGTCCCAGAACAATGCTAAATTTGCTGTCTGCACAGTGGACCGAG TGGTACTTCTGTATGATGAACATGGGGAGCGGAGAGACAAGTTCTCCACCAAACCAGCTGACATGAAGTA CGGCAGGAAAAGCTACATGGTGAAAGGCATGGCTTTCTCTCCCGATTCCACAAAAATTGCCATAGGACAG ACTGACAACATCATCTATGTCTACAAGATTGGAGAAGATTGGGGAGACAAGAAGGTCATCTGCAACAAGT TCATCCAGACGAGTGCTGTGACTTGTCTGCAGTGGCCTGCGGAATACGTCATTGTCTTTGGACTGGCAGA AGGCAAGGTTCGCTTAGCAAACACTAAAACTAACAAGTCATCTACTATCTATGGGACAGAGTCTTACGTG GTAGCACTGACAACCAACTGCTCTGGGAAGGGGATCCTCTCGGGTCACGCAGACGGCACCATCGTCAGGT ACTTCTTCGATGATGAAGGCTCTGGAGAGTCACAGGGAAAGTTGGTTAACCATCCATGCCCACCCTATGC CTTGGCCTGGGCCACCAACAGCATTGTGGCCGCTGGCTGTGACCGGAGGATTGTGGCCTATGGAAAGGAA GGCCATGTGCTGCAGACTTTTGACTATAGCCGAGACCCTCAGGAGCGGGAGTTCACCACAGCTGCTGCAA GTCCTGGAGGCCAGTCTGTTGTGCTGGGAAGTTATGACAGACTTCGGGTGTTCAACTGGAGTCCTCGAAG AAGCATCTGGGAAGAGGCGAAACCCAAGGAGATCGCTAACTTATACACCGTCACCGCCCTGGCCTGGAAG CGGGATGGCTCACGACTCTGTGCGGGAACACTCTGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAA GGAGCATTTACAAGAACAAGTTCGAGCTGACGTATGTGGGCCCTAGCCAGGTGATTGTGAAGAACCTGTC TTCAGGGACCAGGGTGGTGCTCAAGTCACACTATGGCTATGAAGTAGAAGAGGTGAAAATCCTGGGAAAG GAACGTTACTTGGTGGCTCACACATCAGACACACTGCTGCTGGGAGACCTGAACACGAACCGCCTCAGCG AGATAGCCTGGCAGGGATCTGGGGGCAATGAGAAATATTTCTTTGAAAATGAGAATGTGTGCATGATCTT CAATGCTGGAGAGCTGACCCTGGTGGAATATGGGAGTAACGACTCCTTGGGTTCTGTGCGCACCGAATTC ATGAACCCTCACCTCATCAGCGTCCGTATCAATGAGCGCTGCCAGCGAGGAATGGAAGATAATAAGAAAT TGGCTTATCTTGTTGATATTAAGACAATTGCTATAGTGGATCTGATTGGTGGCTACAACATTGGTACTAT CAGTCACGAAAGCCGGGTGGATTGGCTAGAACTTAACGAGACTGGACACAAGCTTCTCTTTAGAGACCGG AAACTTCGGCTGCATCTATATGATATTGAAAGCTGCTCTAAGACAATGATCCTCAACTTCTGCTCCTACG TGCAGTGGGTTCCGGGGAGTGATGTGCTGGTAGCTCAGAACCGAAACAGCTTATGTGTATGGTACAATAT TGAGGCCCCTGAGAGAGTCACCATGTCCTCTATCAGGGGTGATGTTGTCGGTCTGGAGCGAGGTGGTGGG AAGACAGAGGTCATGGTGACAGAAGGCGTGACTACTGTGGCCTACACCCTGGACGAGGGCCTGATTGAGT TTGGAACAGCCATCGATGATGGCAACTACACCCGGGCTACAGCCTTCTTGGAGACCCTGGAGATGACTCC CGAAACAGAGGCAATGTGGAAAACCTTGAGCAAACTGGCCCTGGAGGCAAGGCAGCTTCACACTGCTGAG AGGTGCTTCTCTGCTTTAGGTCATGTGGCCAAAGCTCGGTTCCTGCATGAGACCAATGAGATTGCAGATC AGGTGTCCAGGGAATATGGTGGAGAAGGAACGGACTTCTATCAGGTCCGAGCTCGTCTCGCCATGCTGGA GAAGAACTACAAACTTGCTGAGATGATCTTCTTGGAACAGAATGCTGTTGAGGAGGCCATGGACATGTAC CAAGAGTTGCACCGCTGGGAGGAATGCATTGCTGTGGCTGAGGCCAAGGGACACCCAGCCCTGGAGAAGC TGCGCAGAGACTACTATCAATGGTTAATGGACACACAGCAAGAGGAACGAGCAGGTGAACTGCAGGAGAG CCAAGGGGATGGGCTGGCAGCTATCAGCCTCTACCTCAAAGCTGGGCTCCCTGCCAAAGCTGCGCGGCTG GTGCTGACCCGAGAGGAGCTGCTGGCCAACACAGAACTAGTAGAACATATCACCACAGCCCTCATCAAGG GAGAGCTCTATGAAAGGGCAGGTGATCTCTTTGAGAAGATTCGAAATCCACAGCGGGCCCTTGAGTGTTA CTGTAAAGGCAATGCATTCATGAAAGCTGTTGAGCTGGCTCGACTGGCCTTCCCAGTGGAGGTGGTGAGG CTAGAGGAGGCCTGGGGGGACCACTTGGTACAGCAAAAGCAGCTGGATGCGGCCATCAACCACTATATTG AAGCCAGGTGCTCCATCAAAGCAATTGAGGCTGCCCTGGGTGCCCGCCAGTGGAAGAAGGCAATTTATAT ATTAGATCTCCAGGATCGCAACACTGCATCTAAATACTATCCTCGTGTGGCTCAACACTATGCGTCTCTG CAGGAGTATGAGATTGCTGAGGAGCTCTACACCAAAGGAGACCGGACCAAAGACGCCATAGACATGTACA TCCAGGCCGGCCGCTGGGAGCAAGCCCACAAGCTGGCCATGAAATGCATGCGACCCGAGGACGTGTCCGT GCTTTACATCACACAAGCCCAGGAGATGGAGAAGCAGGGCAAGTACCGAGAGGCTGAGAGGCTGTACGTG ACGGTGGAGGAGCCTGATCTCGCCATCACCATGTTCAAAAAGCACAAGTTGTATGACGACATGATTCGCC TGGTGGGGAAGCACCATCCAGACCTCCTCAGCGACACACACCTTCACCTGGGCAAGGAGCTGGAGGCAGA AGGCCGACTGCAGGAGGCTGAGTATCACTACCTTGAGGCCCAGGAATGGAAGGCAACTGTGAACATGTAC CGCTCCAGCGGGCTTTGGGAGGAGGCCTACCGGGTGGCCAAAGCCCATGGAGGAGCCAATGCCCACAAAC ACGTGGCCTATCTGTGGGCCAAAAGCCTGGGAGGAGAGGCTGCAGTTAGACTACTTAACAAGCTGGGACT TCTGGAGGCCACCATTGACCATGCTGCTGACAACTGCTCCTTTGAGTTTGCCTTTGAACTCTCTCGGCTA GCCTTCAAGCACAAAGCCCCAGAGATTCATCTCAAATACGCCATGTACCTGGAAGATGAGGGCAAATTTG AAGAGGCTGAAGCTGAATTCATCCGAGCTGGTAAACCCAAAGAGGCGGTCCTCATGTTTGTCCACAACCA GGACTGGGAGGCAGCTCAGCGCGTGGCTGAGGCCCACGATCCTGACAGCGTTGCTGAGGTACTTGTAGGT CAAGCCCGTGGGGCCTTGGAGGAGAAGGACTTTCAGAAGGCAGAAGGGTTACTACTTCGGGCCCAGAGAC CAGGTCTGGCTCTCAATTATTATAAGGAGGCTGGACTCTGGAGCGACGCTCTGAGAATCTGCAAGGACTA TGTGCCTGGCCAGCTAGAGGCCCTACAGGAAGAGTATGAGCGAGAAGCTACCAAGAAGGGAGGCAGAGGG GTGGAGGGACTTGTAGAGCAAGCACGGCAGTGGGAACAGGCTGGAGAGTATAGCCGTGCAGTGGACTGCT ACCTCAAAGTTCGCGACTCAGGAAGCAGTGGCCTGATGGAGAAGTGCTGGATGAAGGCGGCTGAACTGTC CATCAAGTTCCTGCCTCCCCAGCGCAGTCTGGAAGTCGTCCGGGTTGTAGGGCCCCAGCTGATCGGGATT GGAAAGCACAGTGCTGCTGCAGAACTCTATCTGAACCTGGACCTTGTCAAAGAAGCAATCGATGCTTTCA TTGAGGGGGAAGAGTGGAACAAGGCCAAGCGCGTGGCCAAAGAGCTAGATCCCCGGTATGAGGACTATGT GGATCAGCACTATAAGGAGTTCCTCAAGAACCAGGGCAAAGTGGATTCGCTGGTGGGTGTGGACGTAGTA GCTGCCTTGGACCTGTATGTGGAGCAGGGCCAGTGGGATAAATGCATTGAAACAGCTACCAAGCAGAACT ACAAGATTCTGCACAAGTATGTGGCTTTGTATGCAACTCATCTGATCCGAGAAGGTGGCTATGCCCAGGC ACTGGCCCTCTATGTGCAACACGGAGCCCCTGCAAACCCACAGAATTTCAACATCTACAAAAGGATCTTC ACTGACATGGTTAGCTCTCCTGGGACCAACAATGCTGAGGCCTACCATAGCTGGGCTGATCTTCGGGATG TCCTCTCCAACCTGTGTGAAAACCTGGTGAAGTCCAGTGAGGCAAACTCTGCAGCGCATGAGGAGTTTGA GATGATGCTGCTGATCTCTCATTACTACGCTACGCGATCTGCAGCCCAGAGTATCAAACAGCTGGAAACC GTAGCTGCCAGGCTTTCTGTTTCGCTCTTGCGCCACACCCAGTTACTCCCTGCAGACAAGGCCTTTTATG AAGCGGGCACTGCTGCCAAGGAAGTTGGCTGGGAGAACATGGCTTTCATTTTCCTCAATCGGTTCTTGGA TCTGACTGATGCAATCGAAGAAGGGACCCTGGATGCCCTGGACCATTCAGATTTTCAGGATACTGACATT CCCTTTGAGGTGCCGCTCCCAGCCAAGCAGCACGTCCCGGAGGCCCAGAGAGAAGAGGTCCGAGACTGGG TGCTCACAGTCTCGATGGACCAGAGACTGGAGCAGGTTCTGCCCCGGGATGAGCGAGGTGTCTATGAGGC TTCCCTAGTTGCCGCGAGCACTGGAGTTCGGGCACTGCCCTGCCTCATCACAGGTTACCCCATTTTGAGG AACAAAATTGAATTTAAGCGGCCTGGGAAGGCTGCTAACAAAGACAACTGGAACAAGTTTCTTATGGCCA TCAAGACCTCTCACAGCCCAGTGTGCCAGGACGTGCTCAAGTTCATCAGCCAGTGGTGCGGTGGTCTCCC TAGCACCAGCTTTTCCTTTCAGTGACTGGCGCAGCCGAGACGCTTACATCTGTCCCTCATTAAAGTTCTC TGAGTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6856438 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6856438 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12294 same embryo
	EMAGE:12293 same embryo
	EMAGE:12297 same embryo
	EMAGE:12295 same embryo
	EMAGE:12296 same embryo
	EurExpress:euxassay_010945 same experiment
	MGI:4825530 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source