EMAGE mouse gene expression spatial database

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EMAGE:12812

2400003C14Rik RIKEN cDNA 2400003C14 gene ( MGI:1919205)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013623. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013623_01	euxassay_013623_02	euxassay_013623_03	euxassay_013623_04

euxassay_013623_05	euxassay_013623_06	euxassay_013623_07	euxassay_013623_08	euxassay_013623_09

euxassay_013623_10	euxassay_013623_11	euxassay_013623_12	euxassay_013623_13	euxassay_013623_14

euxassay_013623_15	euxassay_013623_16	euxassay_013623_17	euxassay_013623_18	euxassay_013623_19

euxassay_013623_20	euxassay_013623_21	euxassay_013623_22	euxassay_013623_23	euxassay_013623_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12812_wholemount_strong.wlz

12812_wholemount_moderate.wlz

12812_wholemount_weak.wlz

12812_wholemount_possible.wlz

12812_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12812_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 12 21 22 weak expression: see section 19 20

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 07 08 09 10 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 06 07 08 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 10 22

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 11 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 09 22

trigeminal v nerve

moderate

regional

moderate expression: see section 11 19

spinal cord

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 10 12 13 14 19 20 21

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05

cornea

moderate

regional

moderate expression: see section 04 05

neural retina

moderate

regional

moderate expression: see section 01 02 03 04

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

lower jaw molar

weak

regional

weak expression: see section 08 09 21 22

upper jaw incisor

weak

regional

weak expression: see section 13 14 17 18

upper jaw molar

weak

regional

weak expression: see section 08 09 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T850
Entity Detected:	2400003C14Rik, RIKEN cDNA 2400003C14 gene ( MGI:1919205)
Sequence:	sense strand is shown >T850 TCCTCGAGNCTGTTGGCCTACTGGTTTTTGGATGCCGAGCGGCTCCTCGGAAGTCGGCTTCTTCCGTTTT CACGTCCGGATCCCTCCAGGAGGAGCAGGACAACATGCTGGGCTCTGGGTTCAAAGCTGAGCGCTTACGA GTCAATTTGAGATTAGTTATAAATCGTCTCAAACTGTTGGAGAAAAAAAAAACGGAACTGGCTCAGAAGG CAAGGAAGGAGATTGCTGACTACCTGGCCGCCGGAAAGGATGAGCGAGCACGCATCCGTGTGGAGCACAT AATCCGGGAGGACTACCTCGTGGAGGCCATGGAGATCCTGGAGCTGTATTGTGACCTGCTGCTGGCGCGC TTTGGCCTTATCCAGTCTATGAAGGAGCTAGATTCTGGTCTAGCTGAATCCGTGTCTACACTGATCTGGG CTGCGCCTCGGCTTCAGTCAGAAGTGGCTGAGTTGAAAATAGTTGCCGATCAGCTCTGTGCCAAATACAG CAAGGAATATGGCAAGTTGTGCAGGACCAACCAGATCGGGACTGTGAATGATAGGCTAATGC
Notes:	The probe template was PCR amplified from IMAGE:1923024 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1923024 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12813 same embryo
	EMAGE:12811 same embryo
	EurExpress:euxassay_013623 same experiment
	MGI:4822670 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source