EMAGE mouse gene expression spatial database

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EMAGE:14182

Apoe apolipoprotein E ( MGI:88057)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003949. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003949_01	euxassay_003949_02	euxassay_003949_03	euxassay_003949_04

euxassay_003949_05	euxassay_003949_06	euxassay_003949_07	euxassay_003949_08	euxassay_003949_09

euxassay_003949_10	euxassay_003949_11	euxassay_003949_12	euxassay_003949_13	euxassay_003949_14

euxassay_003949_15	euxassay_003949_16	euxassay_003949_17	euxassay_003949_18	euxassay_003949_19

euxassay_003949_20	euxassay_003949_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14182_wholemount_strong.wlz

14182_wholemount_moderate.wlz

14182_wholemount_weak.wlz

14182_wholemount_possible.wlz

14182_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14182_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 17 moderate expression: see section 07 08 09 10 11 16 18 19 20

liver left lobe

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4968
Entity Detected:	Apoe, apolipoprotein E ( MGI:88057)
Sequence:	sense strand is shown >T4968 GGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGCTCTGTGGGCCGTGCTGT TGGTCACATTGCTGACAGGATGCCTAGCCGAGGGAGAGCCGGAGGTGACAGATCAGCTCGAGTGGCAAAG CAACCAACCCTGGGAGCAGGCCCTGAACCGCTTCTGGGATTACCTGCGCTGGGTGCAGACGCTGTCTGAC CAGGTCCAGGAAGAGCTGCAGAGCTCCCAAGTCACACAAGAACTGACGGCACTGATGGAGGACACTATGA CGGAAGTAAAGGCTTACAAAAAGGAGCTGGAGGAACAGCTGGGTCCAGTGGCGGAGGAGACACGGGCCAG GCTGGGCAAAGAGGTGCAGGCGGCACAGGCCCGACTCGGAGCCGACATGGAGGATCTACGCAACCGACTC GGGCAGTACCGCAACGAGGTGCACACCATGCTGGGCCAGAGCACAGAGGAGATACGGGCGCGGCTCTCCA CACACCTGCGCAAGATGCGCAAGCGCTTGATGCGGGATGCCGATGATCTGCAGAAGCGCCTAGCTGTGTA CAAGGCAGGGGCACGCGAGGGCGCCGAGCGCGGTGTGAGTGCCATCCGTGAGCGCCTGGGGCCTCTGGTG GAGCAAGGTCGCCAGCGCACTGCCAACCTAGGCGCTGGGGCCGCCCAGCCTCTGCGCGATCGCGCCCAGG CTTTTGGTGACCGCATCCGAGGGCGGCTGGAGGAAGTGGGCAACCAGGCCCGTGACCGCCTAGAGGAGGT GCGTGAGCACATGGAGGAGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATACGCCTGCAGGCGGAG ATCTTCCAGGCCCGCCTCAAGGGCTGGTTCGAGCCAATAGTGGAAGACATGCATCGCCAGTGGGCAAACC TGATGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCATCACCCCAGTGGCCCAGGAGAATCAATG AGTATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTGGCCCTGTCTCAAGCACCTCTCTGG CCCTCTGGTGGCCCTTGCTTAATAAAGATTCTCCGAGCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136415 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136415 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14183 same embryo
	EMAGE:14180 same embryo
	EMAGE:14179 same embryo
	EMAGE:14184 same embryo
	EMAGE:14181 same embryo
	EurExpress:euxassay_003949 same experiment
	MGI:4823189 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source