EMAGE mouse gene expression spatial database

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EMAGE:15816

Cacna1i calcium channel, voltage-dependent, alpha 1I subunit ( MGI:2178051)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013223. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013223_01	euxassay_013223_02	euxassay_013223_03	euxassay_013223_04

euxassay_013223_05	euxassay_013223_06	euxassay_013223_07	euxassay_013223_08	euxassay_013223_09

euxassay_013223_10	euxassay_013223_11	euxassay_013223_12	euxassay_013223_13	euxassay_013223_14

euxassay_013223_15	euxassay_013223_16	euxassay_013223_17	euxassay_013223_18	euxassay_013223_19

euxassay_013223_20	euxassay_013223_21	euxassay_013223_22	euxassay_013223_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15816_wholemount_strong.wlz

15816_wholemount_moderate.wlz

15816_wholemount_weak.wlz

15816_wholemount_possible.wlz

15816_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15816_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11 12 15 weak expression: see section 07 08 09 16

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 16

rest of cerebellum mantle layer

weak

regional

weak expression: see section 07 16

pons mantle layer

moderate

regional

moderate expression: see section 11 weak expression: see section 05 06 07 13 14 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39580
Entity Detected:	Cacna1i, calcium channel, voltage-dependent, alpha 1I subunit ( MGI:2178051)
Sequence:	sense strand is shown >T39580 CCATCTTCCACCACTACTCCTCACCTGCCGGCTGTGACAAGTGTCACCATGACAAGCAAGAGAAGGAAGG GGGGTCTCTGGTGTTTGCTTGTTGTTGTTCTCTGATGGTCATGGCCACCCCCACCCCACCCCCACCCCAA GTATCTCGGTGCATCCCTCGAGAGCCTCCTTTAGGCTTCGTCTCCGTGGCAGCTCAGACCCACTCTGCTC AGGACCCATCTCTCAAGGCTGGACCTGTTGCTCAGCGCTCCAGGCCCAGGCTGCCCCATCCCAGCCCCAG GGTGACCCGAGGCCACCACCTCCCCCCGCAGGTGCAGCTGGCTGAGACAGAGGCCTTCTCCCTGAACTCA GACAGGTCCTCGTCCGTCCTGCTGGGGGATGACCTGAGTCTTGAGGACCCCACGGCCTGCCCGCAGGGCC CCAAGGAGAGCAAGGGTGAGCTAGAGCCTCCGGAGCCCATGCAGGCTGGAGACCTGGATGAATGCTTCTT CCCCTTTGCCGGTGAGCCAGTGTCTGCAGGCCCAGAGAGCCTGCTGTGCGAGATGGGGGCCATTCCATTC AACCCCGTCCAGTCCTGGCTCAAACATGAGAGCAACCAAGCACCCCCAAGCCCTTTCTCCCCGGATGGTT CCAGCCCTCTGCTGCAGATGCCTGCTGAGTTCTTCCACCCTGCTGTGTCTGCCAGCCAGAAGGGGCAGGA ACCGGGCATGAGTTCAGGGACCCTGCCCAAGATTGCACTTCAGGGGTCCTGGGCATCCCTGAGGTCACCG AGTGTCAACTGCACCCTCTTGCGCCAGGCTACTGTGAGTGACACGTCTTTGGATGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 112407. Forward Primer - name:112407_F_cDNA_LOC239556, sequence:CCATCTTCCACCACTACTCCTC; Reverse Primer - name:112407_N_SP6_cDNA_LOC239556, sequence:GGCATCCAAAGACGTGTCACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15815 same embryo
	EMAGE:15817 same embryo
	EMAGE:15820 same embryo
	EMAGE:15818 same embryo
	EMAGE:15819 same embryo
	EurExpress:euxassay_013223 same experiment
	MGI:4823577 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source