EMAGE mouse gene expression spatial database

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EMAGE:17392

Acsm4 acyl-CoA synthetase medium-chain family member 4 ( MGI:2681844)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003695. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003695_01	euxassay_003695_02	euxassay_003695_03	euxassay_003695_04

euxassay_003695_05	euxassay_003695_06	euxassay_003695_07	euxassay_003695_08	euxassay_003695_09

euxassay_003695_10	euxassay_003695_11	euxassay_003695_12	euxassay_003695_13	euxassay_003695_14

euxassay_003695_15	euxassay_003695_16	euxassay_003695_17	euxassay_003695_18	euxassay_003695_19

euxassay_003695_20	euxassay_003695_21	euxassay_003695_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17392_wholemount_strong.wlz

17392_wholemount_moderate.wlz

17392_wholemount_weak.wlz

17392_wholemount_possible.wlz

17392_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17392_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7771
Entity Detected:	Acsm4, acyl-CoA synthetase medium-chain family member 4 ( MGI:2681844)
Sequence:	sense strand is shown >T7771 CAGAAAAGAAGCAACTTTGTGGCAGAAACAGCCTTTGGGAAGGAGCGGGAGGCCTTTCTGGATCAGCAGG ACCATTTGCTGGCATTTCCCACTTGCTACCAGAAAATAAAGTTTTTCAGGAACCATGAAGGTTCTCCTCC ACTGTCAGAGACTTAGATTCATCTGGCTTGCCAAGCCAGCCGGCCGGCACTTCCACAGAGATTCCCAGCT CTGGGCACCTCTGACACTGGATGACTTTGAAGCCATCAATCGCTGTGAGAAGCCATTACCCAAGAACTTT AACTTTGCAGCAGATGTTCTGGACCAGTGGTCCCTAAAGGAGAAGTCTGGAGAGAGGCCAGCCAACCCAG CCCTGTGGTGGGTGAATGGGAAAGGAGATGAGGTGAAGTGGAGCTTCCAAGAACTGGGTTCCTTGTCCCG AAAGGCTGCCAATGTGCTCACCAAGCCCTGCGGCCTGCAGAGAGGAGACCGGGTGGCTGTGATTCTGCCC CGTATCCCTGAATGGTGGCTCATCAATGTGGCTTGCATGAGAACAGGGCTTGTATTCATGCCAGGAACGA TCCAACTGACCAGAAAAGACATCCTCTACCGGCTGCAAGCATCCAAGGCCAAGTGCATTGTGGCTAGTGA GGAAGTGGCCCCCGCAGTGGACTCCATTGTATCAGAGTGTCCCAGCCTGAAGACTAAACTCCTGGTGTCT CCACACCACTGGGATGGATGGCTCAACTTTCAGGAGTTACTTCAATCGGCCTCTGAAGAGCACAATTGTG TGGAGACGGGAAGTCAAGAACCAATGGCCATTTACTTCACCAGTGGGACCACAGGCTCCCCCAAGATGGC TCAGCACTCACAGGGCAGCCTTGGCATTGGGTATACCCTTTGTGGAAGGTACTGGCTGGACTTGACATCC TCAGATATCATGTGGAACATGTCAGACACTGGCTGGATCAAGGCTGCCATTGGTAGTGTGTTTTCTACAT GGCTTCGAGGGGCCTGTGTTTTTGTGCACCGGATGGCCCAGTTCGACACTGATATCTTCCTAGACACACT TACCACATATCCCATCACGACCCTGTGCAGTGCTCCTACGGTGTACCGGATGCTCGTACAAAAGGACCTT AAGAGATATCAATTCAAGAGGCTGCGGCACTGCCTGACAGGAGGGGAGCCACTCAACCCCGAAGTGCTGG AACAGTGGAAGATGCAAACTGGGCTGGAGCTGTACGAGGGCTACGGACAGACCGAAGTGGGAATAATTTG CGCCAATCGCAAAGGAGAAGCAATTAAACCAGGTTCCATGGGAAAAGGAGTAGTGCCGTACGATGTGCAG ATTATAGATGAAAATGGAAATATCCTACCATCCGGCAAGGAAGGGGAAATTGCCCTTAGGCTGAAGTCCG ATCGGCCCTTCTGTTTCTTCTCTGAATATGTGGACAATCCAGAAAAGACAGATGCCACAATAAGAAGAAA TTTTTATGTCACTGGAGACAGAGGAGTGATGGATGATGACGGATACTTTTGGTTTGTCGGCAGAGCTGAT GATGTCATTATATCTTCTGGGTACCGAATTGGGCCTTTTGAAGTAGAGAGCGCACTGATAGAGCACCCAG CAGTAGTTGAGTCAGCTGTTGTCAGCAGTCCAGATCCGATCCGAGGAGAGGTGGTGAAGGCATTTATTGT ACTAGCTGCACCCTACAAGTGCTCCAACCGTGAGAAGTTAACCGCTGAACTTCAGGATCATGTGAAAAAT TCAACAGCACCTTACAAATACCCAAGAAAAGTGGAATTTGTTCAAGAACTCCCAAAGACAATCACTGGGA AAATCAAACGCAACGTGTTAAGAGACCAGGAATGGGGAAGAGCATAGCTGGATGAGAACCTTGCTAGAAG TGATGCGGTTGGTTTCAGTACCAGCTTCCTGATAGTTCAACGGCTACTCTTAAAATGCTTGAACATTTTT TTTCCTATTTCACTCTTAAAATGCTTTGTCTTTCCTGTTTCGCTCTTAAAATGCTTTAACACTTGTTTTT TTCCTGCTTGAAAATAGTTCTATCTAGGAAAACAGTAAAGAAACAACCAAAATCTAAAAAACAAAAAAAA AAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:6312894 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312894 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17393 same embryo
	EMAGE:17391 same embryo
	EMAGE:17390 same embryo
	EurExpress:euxassay_003695 same experiment
	MGI:4822951 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source