EMAGE mouse gene expression spatial database

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EMAGE:19770

Tgfbr1 transforming growth factor, beta receptor I ( MGI:98728)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006306. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006306_01	euxassay_006306_02	euxassay_006306_03	euxassay_006306_04

euxassay_006306_05	euxassay_006306_06	euxassay_006306_07	euxassay_006306_08	euxassay_006306_09

euxassay_006306_10	euxassay_006306_11	euxassay_006306_12	euxassay_006306_13	euxassay_006306_14

euxassay_006306_15	euxassay_006306_16	euxassay_006306_17	euxassay_006306_18	euxassay_006306_19

euxassay_006306_20	euxassay_006306_21	euxassay_006306_22	euxassay_006306_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19770_wholemount_strong.wlz

19770_wholemount_moderate.wlz

19770_wholemount_weak.wlz

19770_wholemount_possible.wlz

19770_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19770_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

vomeronasal organ

strong

regional

strong expression: see section 12 13 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8814
Entity Detected:	Tgfbr1, transforming growth factor, beta receptor I ( MGI:98728)
Sequence:	sense strand is shown >T8814 CGGGACCATGGAGGCGGCGGCCGCTGCTCCACGTCGTCCGCAGCTCCTCATCGTGTTGGTGGCGGCGGCG ACGCTGCTCCCGGGGGCGAAGGCATTACAGTGTTTCTGCCACCTCTGTACAAAGGATAATTTTACCTGTG AGACAGATGGTCTTTGCTTTGTCTCAGTCACTGAGACCACAGACAAAGTTATACACAATAGTATGTGTAT AGCTGAAATTGACCTAATTCCTCGAGACAGGCCATTTGTATGTGCACCATCTTCAAAAACAGGGGCAGTT ACTACAACATATTGCTGCAATCAGGACCACTGCAATAAAATAGAACTCCCAACTACAGGACCTTTTTCAG AAAAGCAGTCAGCTGGCCTTGGTCCTGTGGAGCTGGCAGCTGTCATTGCTGGTCCAGTCTGCTTCGTCTG CATTGCACTTATGCTGATGGTCTATATCTGCCATAACCGCACTGTCATTCACCACCGTGTGCCAAATGAA GAGGATCCATCACTAGATCGCCCTTTCATTTCAGAGGGCACCACCTTAAAAGATTTAATTTATGATATGA CAACATCAGGGTCTGGATCAGGTTTACCACTGCTTGTTCAAAGAACAATTGCCAGGACCATTGTGTTACA AGAAAGCATTGGCAAAGGTCGGTTTGGAGAAGTTTGGCGAGGCAAATGGCGGGGAGAAGAAGTTGCTGTG AAGATATTCTCTTCTAGAGAAGAGCGTTCATGGTTCCGAGAGGCAGAGATTTATCAGACTGTAATGTTAC GCCATGAAAATATCCTGGGATTTATAGCAGCAGACAACAAAGACAATGGGACATGGACGCAGCTGTGGTT GGTGTCAGATTATCATGAGCATGGATCCCTTTTCGATTACTTGAATAGATACACTGTTACTGTGGAAGGA ATGATCAAGCTTGCTCTGTCCACAGCAAGTGGTCTTGCCCATCTTCACATGGAGATTGTTGGTACCCAAG GAAAACCAGCTATTGCCCATAGAGATTTGAAATCAAAGAATATCTTGGTGAAGAAAAATGGAACCTGTTG TATTGCAGACTTGGGACTTGCTGTGAGACATGATTCTGCCACAGATACAATTGATATTGCTCCAAACCAC AGAGTAGGCACTAAAAGGTACATGGCCCCTGAAGTTCTAGATGATTCCATAAATATGAAACATTTTGAAT CCTTCAAACGCGCTGACATCTATGCAATGGGCTTAGTGTTCTGGGAAATTGCTCGACGCTGTTCTATTGG TGGAATCCATGAAGACTATCAGTTGCCTTATTATGATCTTGTACCTTCTGATCCATCGGTTGAAGAAATG AGAAAAGTAGTTTGCGAACAGAAGTTAAGGCCAAATATTCCAAACAGATGGCAGAGCTGTGAGGCCTTGA GAGTGATGGCTAAAATTATGAGAGAATGCTGGTATGCCAATGGAGCAGCAAGGCTGACAGCTTTGCGAAT TAAAAAAACATTGTCACAACTCAGCCAACAGGAAGGCATCAAAATGTAACTGAAACACCGTGGGAACTCT GCTCTCTTCATATCTGCTCCTGGGTGTTTAGGAGGCTGGTTGTTCTACCTCACTGAGAGAACAGAGGGCT CTGCTTCCTCTTGCAGCAGTGGAATATGGTCAACTGAAAGCTTCCCAGGGTTTCTCTGGGCCCAGAGGCA GCCGTGGGGTCCTTTCTGTGCACTATGGATACTTCTTCCAGACAGTTACAGAATGTTGTGTAGTCTACTT TTGTTTTTATTAACAAAGCTTGTTTTTAAAGACAACTGCCAGCCCTTAGGGAGCTGCTGTGCTGTTGACC TTCTGTAAGGGTGAAGGAGCTGGGGTGTGGCACTAAATGAAGCACTTCTGATTTTCACTCCTAGTAGTAC ATTCTCAGAGGACTCTGAACCACTAGTGTTTCCTTGGTTCACTTTGAATGTACTGTTCTATAATTTTTCA AGATCTTAAACTAACACTTTAAACTCTATAGTCTAAAAAATATGACCTCGTGGAGGCGTGGGGGCACAGT TGCTCCGTTGTATTTGTGCACAGCTCCTTCACACTCTGAACACAACACACCTTCACAGTAGGGTTGTGCT ACACCAGTAAGTGCTACTTTTGCATCTGTCTAAGTGGAAACGAACAGAACTACTTAAAGGCGGTGTTAAG TCCTGTATCATTTTCATTCAAAAAGTTTATTTTTCTGAGTAACCTAAACCTTTTCTGGGTTTGTTTTTGT TTTTTGAGGGTTTTTGTGATAGATGTCATTCAGTCTTCATGCCTTTCTCCTACCCAGATGTGCTTGAACC ACTGAGGCAAGGCTGTAGCATTGATAAGCAGTGACTGCCATGCGGGAACACCCCACAGGAGCACTGGAGC TCTGCAGTTGAGACGTTTAGCCTGAGTTTATTTTAAGAAGTGAAGTGGCACTCCAAACATGTACAGTGCT TTATGGATGTAATCATTATGGTATACAGACTCCTTTTGAAAGGGGAGGCTTGTCTATTGCCGAAGGAAAA GTTGTATGCTGTTCTGTAAGCCATTTCTTTTTCTTTATTTGATCAAAGCAGTGTTTTGTTTTGTTTTGAT TTTTCTTTTTTGGAAAGGAATTGCATTTAAAATTCAAATGTGGTTAATGTTAAATAATAGGCCTTTTTCT AGGAAGGCAGATATAGTTAATATGAATGTACAAGTGTAGGTGACTTTCACATGTTATGTAGCTGTAAGTA TTTGTGGATTCTATCTTGGGAAGGGTAGAGTTAGTGGAGTTTTGAGGTCTCACTACCCTTTGAGGAAGGC AGCTTTTAATTCAGTCTTTTTATCTCATATGTGCAGATCTTGCAACTGCTTGATTTACATGGTAGCAATG AATTCAGTGCCACGTTGATACTAGGAGAAGCAGCAACTAATAGCTATTCTGAGTAGAGGCTCTCTTCATA TATTCTTGGTCATATTAAATGTCAAAGTCAGTCCGTTGGGTCTTCTCACTGCTGCTGGAGTTAGGTGGTT GAGCCTGGGTTTTACTTCAGTGCTGTCAAATCTTTGCAGGGCTTGTGTGGCTTTCAAAGTCCTTTTTATC ACTGTGATCATATTCTGGGCGGGGAAATGTTATCATCATTGCTTGAGACGAGATGTTGAACATAGTGATA CCAATCCCCAGATAAAAAGGTCAAAATAACCTTTAGTGTTTAGCCAAGAAGAGATTGTGACTATTGATCT GAAGCAGCTGGGCTCTGTTAATGTCTTATTTCAAACCTAGCATACATTTGAAGACTATTTGGTCTTAAAC CAAAGTAACTGTAGGACGAATGACATACTACCTAGACATTAGTGTCCGTTTCATACGTCAGAAACACCAT GGGAAAAAGTACCTAGAGATTAGTATCTTTAATACAAATCTGAGTTTTTTCTGTAATTATCATGATTTCA TCAATGTAAGTAAGGGTTTGTTGTTACAGTTGTATCACCTGCTTAAATAAGCTCTTGTGCTATAATCAGG TACTTTTGGGGCCTTTGTTTTCAGTTTTTTCCATTTCTAACCCTTCGAGATCTGCTTTATAGAAATCCAG GGACCAATGTATTTTATCACTAAAACTGTTTTTATATAATTTTAAGACCATACCAAAAGTTATCTGAGTT AAAGTTGTAATACGTTTCTTACTTTCTTGCAAGGAAATGGGTTTTTACTGAAGACACTTCTCATGATATC TGAAGTTGAAGAATCATTATGTTTTTCTTACCTAAGTGGATAAAATGTGCTTTGATGAATCAGGGAATTT TTTAAAGTTCGAATTTAGTTCTAAATTGAGTTTACATGTTTACAGCTAATTTTTTGTGTAGGGATAGTTT GATTCTCTAGGTGGCCAATACTGAATATAAAAATAACTTAAAAATTGAGATAGGTCATGGTTAATGTGGC TATAACTATATATACATCTAATTAGAGAAATATTTCTATGTAATTTTGTAGTGCAGTCTGTTGTTTCTAT TGCCAATCAAAGACTTCCCCCCAGCCCCAAAGTGTAGTATGGCTAAGACATATCTTCTGACAAATTACAT ATTATCTTGTCAATACCCTGGTTAAGCTAGCATGAAGGAAGTATGACTTACTGAGGCTTACACAACTTGA GTCCCTTATATCTTCAGCTCTGTTTTTCCCACTCTGCCTTTCTGCTGCCCCCTGGCTCAGAGACTTAGTG TTCCTGACCCCACCTCCAGCCTTTGGACCTAAGAGCTGCTTTTGAGACCTAAAATGAAGTGATTGATGGA AGCTGTGTGACATTTTTCCACTTCCTTGAGTCACTGGGTGTTATGAGGGAGTGATCTGCAAATTGGGGTA GGGGGTGCCCCTACTGGTAGCCCTGCTTTGATGTCAGCTCTGGGCAAAGATTAGGGTGACAGCTAAGTGG CAGTGGAAGTTGGCCTCAGAAGTAGTGGCCAGCTGTGTCTCTAGTAGGACAGTAAAGGCATGAAGCTCAG CCTGTAATCCTGCTACTACAGTAGTACTCCAGAAGTGCCTTGAGGCCCTGTGTGGGGCTGTGGCCATCAA GAATTCCAGATTTTGGTTCTCGCTGCAGACTTAAGTGGCCATGTAGCCATTTTTGTAATCCATACATGTT TGACTCTTCCCTCTGCAAATTGTCAGATACGTAAAACCAATGACAAACTACTACTTAACATGCACACCAA AATCTGCCCAGAAGACATTGTGATTTCATTCCATGCACTGAAGGAATGATTGTAAATCATTTCTTTGTTC CTTGGTCATGGGAGTGTTCTGGTTCTACATTGTGGAGATTCCAGCTGTTGTTCTGTTATAGCCCAGCAGA ACAAGTCGACGTGTTGAAATTCTGAAGAATAGTTTGGGAGTGCAGTACAGCTTCATGTGTTGGATGTTGA AACTTTCATGGGCCCTCTTTAGAAAAGCAGAGGACTGTCAGCACATTATTTTTAATCCTGTTATGCACTC GTACTTATTGTCAGTAACAATAAGTACGAGTATTGAAAATATTGTCAGTAACATTCAGTCTCTTTCTTAA AGTGAATTTGCTTATTTTTCAATGAGATTTGTGAGTTTAATATTATGAACTGTGTTTTGATAATCCCTTT TTCACATTGTGCCAATGGAATGGAATATTTGATATTTCTTTATATGTCAAGGAGATGCTTCAATATGTCA TTTGCTTTAAACTTAAATTACCTCTCAAGAGACCAAGGTACATTTACCTCATTGTGTATATAATGTTTAA TATTTGTCAGCATCCACCAGGTTTGCAATTTTATTTCTATAAAGTGTGAGTGTTACGTTGCTAAGTTACT CAGATGGTACTGTATTGTTTATATCTGTACCCCAAATGACATCATCTAGACTTTGTTTTCTGTGTCTTAT TGTATTTGTGCAGTCCTTTAACCCTGTTGGTGTGATTGCTGCCCTTTCAGATATGTGCAGGTGTCTATAG AAATTCTCATTTAAGGTGAATGGTTTTGAGAAACCTGTGAAGAGAGAAGCATAAACTGCATTTCCCCATC AGTTTTTTAAATGTACATTGTATGTGTAGTAATATTCCAGATGAATTGTAAATAAAAGCTAGGGAGATGC TTAAATTTCATCCTAACACTACAGTAGAAAATGGAAGCAATGCAAATAAACAACTTTTTCCCACCAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409765 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409765 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19771 same embryo
	EMAGE:19767 same embryo
	EMAGE:19769 same embryo
	EMAGE:19766 same embryo
	EMAGE:19768 same embryo
	EurExpress:euxassay_006306 same experiment
	MGI:4828680 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source