EMAGE mouse gene expression spatial database

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EMAGE:7043

Eepd1 endonuclease/exonuclease/phosphatase family domain containing 1 ( MGI:1914734)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002552. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002552_01	euxassay_002552_02	euxassay_002552_03	euxassay_002552_04

euxassay_002552_05	euxassay_002552_06	euxassay_002552_07	euxassay_002552_08	euxassay_002552_09

euxassay_002552_10	euxassay_002552_11	euxassay_002552_12	euxassay_002552_13	euxassay_002552_14

euxassay_002552_15	euxassay_002552_16	euxassay_002552_17	euxassay_002552_18	euxassay_002552_19

euxassay_002552_20	euxassay_002552_21	euxassay_002552_22	euxassay_002552_23	euxassay_002552_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7043_wholemount_strong.wlz

7043_wholemount_moderate.wlz

7043_wholemount_weak.wlz

7043_wholemount_possible.wlz

7043_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7043_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 23

trigeminal v ganglion

strong

regional

strong expression: see section 05 09 moderate expression: see section 06 07 08 10 18 19 20 21 22 23 24

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 16 17 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5096
Entity Detected:	Eepd1, endonuclease/exonuclease/phosphatase family domain containing 1 ( MGI:1914734)
Sequence:	sense strand is shown >T5096 CCACGCGTCCGCCCACGCGTCCGCGTGGCTGTGCGGGCGAAGGCGCGGGCGGGCGGCGGGACCCGACTTC AGCTGTGACACCCACGGCGAGCGGCCGTGGTGGCGCGCGGCGCGAGGTTCAGGACGCGCTCCGCCGCCTC TCGCACCCGAGTTAGGCGCCGGGCTTTGCCTGGATTCAAGCAGCTACGACGCGGCTAGTCCTGGTGCAGC ATCGGGGGCCGAATTTCGCCGCGGCTACTGTCGCTCAGGAGCAATTCTGCCTGCTCCGGGAAAGCGGCAC TGAGAGTTTCCCTGGGAGAAAGAACCGGGACGCGCGCGTAGAGCCACAGGGGACCGGGAACCGATCCGAC CGAGCGGCGGAGAGGTGGCCCAGCAAAAGGTAATTTTGGACACTAGCCATGGAAGATTTGATGTTTGTCT GCAGCAACTTCCTCGACCCTTAAGTCCTGTAACCTCCCGTTCTTTTGTGCCTATCAAGGCTCCCGGGTTT CTTCTCTATCCAGAGTTATTCTGTTGTGGTTGGCCATGGGGAGCACACTGGGCTGCCATCGCTCTATCCC CAGAGATCCCTCGGACCTGTCACATAACCGCAAGTTCAGCGCTGCCTGCAACTTCAGCAACATCCTGGTG AATCAGGAGCGGCTAAATATCAACACTGCCACTGAGGAGGAACTGATGACTCTGCCTGGGGTGACACGTG CAGTGGCCCGAAGCATCGTGGAATACCGAGAATATATCGGCGGCTTCAAAAAGGTGGAAGATCTGGCACT TGTCAGTGGTGTAGGGGCCACCAAACTGGAACAGGTCAAGTTTGAGATCTGTGTGAGCAGCAAGGGTAAC TCTGCACAGCACTCTCCCAGTTCCCTGAGGCGGGATCTTCTGGCTGAGCAGCAGCCTCATCACCTGACCA CCACTGTGCCCCTCACTCCGCGAGTCAATATCAACACAGCCACCCTTGCTCAGCTCATGAGTGTCCGGGG CCTCAGCGAGAAGATGGCCCTCAGCATCGTGGACTACCGCAGGGAACACGGTCCTTTTCGAAGTGTTGAG GACTTGGTGAGAATGGATGGAATCAATGCAGCCTTCCTGGACAGAATAAGACACCAGGTGTTCGCAGAGA GGTCCAGGCCCCCATCAACCCACACCAATGGGGGTCTTACCTTCACTGCCAAGCCTCATCCCAGTCCCAC ATCTCTGAGCCTGCAGAGTGAGGACTTGGACCTGCCACCAGGGGGGCCCACGCAGATTATCTCCATGAGG CCCTCGGTGGAGGCCTTTGGAGGTATGAGGGATGGGCGACCTGTGTTTAGGTTGGCCACGTGGAACTTAC AGGGCTGCTCTGTGGAGAAGGCCAATAACCCGGGGGTTCGAGAGGTGGTGTGCATGACGCTTCTGGAAAA CAGCATCAAACTTCTAGCTGTGCAAGAGCTGCTTGACAAAGAGGCCCTGGAAAAGTTCTGTACAGAGCTG AACCAGCCGATCCTGCCCAACATCCGCAAGTGGAAGGGGTCCCGAGGATGCTGGAGGTCCATTGTTGCTG AGAAGCCTTCCAATCAGCTCCAGAAGGGCCCCTGCTACTCTGGATTCCTATGGGACACAGCAGCCAACGT GGAGCTGAGAGACATCCCCGGGCGGGAGAGCTCACCCAGCAACGGTCATGCTAAGGCTGTGGGCCCCAGC CCTTTCCTCACCAGATTCAAGGTGGGAAGTAATGACTTAACCCTGGTTAACCTCCAGCTGACAGCCCTGG CCCTCCCAGGAGCTGAGAATTCAAGCAAGAATCACAGTGATGGCCACCGATTATTGAACTTTGCACTAAC CCTCCAGGAAACCCTGAAAGGAGAAAAGGATGTTGTTATTTTAGGGGATTTTGGCCAAGGGCCAGATAGC AATGACTACGATATCCTAAGGAGAGAAAAATTTCACCACCTAGTCCCGGCTCACACTTTCACCAACATCA GCACCAGGAATCCTCAAGGCTCCAAGTCTGTGGACAACATCTGGATCAGCAAAAGCTTAAAGAAAGTGTT CACAGGTCACTGGGCTGTTGTGAGAGAAGGGCTCACAAACCCTTGGATTCCAGATAACTGGTCCTGGGGT GGAGTCGCTTCTGAACATTGCCCTGTGCTTGCTGAATTGTACATGGAAAAGGACTGGAGCAAGAAGGAAG TCCCCAGGAATGGCAATGGAGTCACCTTAGAGCCAAGTGAAGCCAACATTAAACATGAGCGATGATGACA TCACTTTTTCCCGCCTCTGACTTGGGAGAGCATAGGATGGAGATGGCACTACAGGGTGAGAAGATCCAGG GCCTGTGAATCATCATTCTGAGGACCTTAGTCTTGGTCCCTGGACCACTGTCCACTTTGTGAACACTTTA GAACCTGGCCTGAGGGGAGCCTGTCAGGCACTGGCTATGGTGGGCATCATGGCGGAGCTGGCTGGAACTG CGACGTCTTGACGCTATGTAGCTGAGTTCGGGCTTGGGATGGGGGCTGCACTGCTGGCTGATTGTTTAGC AGCATGAGAACAGTAACAGAGGAGTGAAGTGCAGATGGGAACAGTGGCTCAGGCTGACAAGCAGTGCCAC AGCTCTCGTTTCTGCCTCACCAGGATGTCATGTTCTACCGACAAATTTCAGGAAGCTTTTAACTGTGATG AATGCACTTCTCAAAGATTTTTAGTTTTGTTTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323841 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323841 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7045 same embryo
	EMAGE:7042 same embryo
	EMAGE:7047 same embryo
	EMAGE:7044 same embryo
	EMAGE:7046 same embryo
	EurExpress:euxassay_002552 same experiment
	MGI:4824477 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source