EMAGE mouse gene expression spatial database

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EMAGE:7087

Cdk20 cyclin-dependent kinase 20 ( MGI:2145349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002618. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002618_01	euxassay_002618_02	euxassay_002618_03	euxassay_002618_04

euxassay_002618_05	euxassay_002618_06	euxassay_002618_07	euxassay_002618_08	euxassay_002618_09

euxassay_002618_10	euxassay_002618_11	euxassay_002618_12	euxassay_002618_13	euxassay_002618_14

euxassay_002618_15	euxassay_002618_16	euxassay_002618_17	euxassay_002618_18	euxassay_002618_19

euxassay_002618_20	euxassay_002618_21	euxassay_002618_22	euxassay_002618_23	euxassay_002618_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7087_wholemount_strong.wlz

7087_wholemount_moderate.wlz

7087_wholemount_weak.wlz

7087_wholemount_possible.wlz

7087_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7087_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5288
Entity Detected:	Cdk20, cyclin-dependent kinase 20 ( MGI:2145349)
Sequence:	sense strand is shown >T5288 TGCTGGATCCCTGTAGAGTAGGATCTGGGCACCGCGGGCCGACATGGACCAGTATTGCATCCTCGGTCGC ATCGGGGAGGGCGCCCACGGCATCGTCTTCAAAGCCAAGCACGTAGAGACTGGCGAGATCGTTGCCCTCA AGAAGGTGGCCTTGCGGCGGCTGGAGGATGGTATTCCTAACCAGGCCCTCAGAGAAATCAAGGCTCTGCA GGAGATAGAGGACAGTCAATATGTGGTGCAGCTGAAGGCTGTGTTCCCACATGGTGCGGGCTTTGTGCTG GCTTTCGAATTTATGCTGTCGGACCTGGCAGAGGTGGTGCGCCATGCCCAGAGGCCCCTGGCCCCAGCAC AAGTCAAGAGCTACCTGCAGATGCTGCTCAAAGGTGTTGCGTTTTGCCATGCCAACAACATTGTGCATCG GGACCTGAAGCCTGCTAACCTGCTCATCAGTGCCTCAGGCCAGCTCAAGATAGCTGACTTTGGCCTGGCC CGGGTCTTCTCTCCGGATGGTGGTCGCCTCTACACACATCAGGTGGCCACCAGGTGGTACCGAGCTCCTG AACTCCTGTATGGCGCTCGGCAGTATGACCAGGGCGTTGACCTATGGGCTGTGGGCTGCATCATGGGAGA GCTGTTGAATGGGTCCCCCCTGTTCCCGGGCGAAAACGACATTGAACAACTGTGCTGTGTGCTTCGCATC CTGGGTACCCCGAGTCCTCGAGTCTGGCCGGAGATCACAGAGCTGCCTGACTACAACAAGATCTCCTTCA AGGAGCAGGCACCAGTGCCCCTGGAGGAGGTGCTGCCTGATGCCTCTCCCCAGGCCTTGGACCTGCTGGG CCAGTTCCTCCTCTACCCTCCACGACAGCGTATTGCAGCCTCCCAGGCCCTTCTGCATCAGTACTTCTTC ACAGCGCCTCTGCCTGCCCATCCATCCGAGCTGCCAATTCCTCAGCGCCCAGGGGGACCTGCACCCAAGG CTCACCCAGGGCCCCCCCATGTCCACGACTTCCATGTGGATCGACCTCTTGAGGAGTCACTGTTGAACCC AGAACTGATTCGGCCCTTCATCCCAGAGGGGTGAGATGCTGGTCCAGGCCTTCCTGCTCGCCCTAGGAGC ACCTCTTTCTGATTTGCCTCCATGGCCTCCCCACGGCTATATATACCACACCTGGTCCTGCTCCTTAGTG TGCTTGAGGGCTGGGCTCTGGGAGGCAGAACCGTGAGATGTTCATCCCAGCAGAGAAAGAGACTCACGTC CTACAGACAAAGCCTCCAGAAACTGCTAGCTGTGTCCTTCTCCAGGGCCACCCCTCAGTGGTGCCACCCG GCCTTAGAGATGATTGTCAGGCTCTGTCCCCTCTTCAAGGACATTGGTACTACAGCACCACCTGGTGGAA GCACAGAGTATAAGCTGTCTTCATACCGGGGACACAGCTGGGAAGTCAGACATGTTTTAGTTTTGGTTCC ACTGGGTCAGGATTTGAGGTTCATATAAAAGCCCTGGGTGTTTCTGTCTAATTGCACCTTGTCTGTTGCT GTTAGGGAAAGGACAATGGTGGGCCTTGATTCACAGGGGTCAGGTACTCAGAAGAGGCCTCCTGTGAAGG CCATTTGGGTCCTCAGGCTTCCCATGCTATTCACGGGACTTGAGTGCTCATTTGGGAGCGAGAGTCCAGA AGCTGAGGCCCAGGGATGGACAGTCCAGTTCCCGAAGCACACTTCCCACATGTCGGGTGGGTCAGTCAGT GAGCCTGAGGCTGCCTTGCAGATGCGGAAGCAGACATTCCTGGAATCCACTCAGTAAATAAATTCCAGTG TGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362198 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362198 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7086 same embryo
	EMAGE:7085 same embryo
	EMAGE:7084 same embryo
	EMAGE:7083 same embryo
	EurExpress:euxassay_002618 same experiment
	MGI:4823778 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source