EMAGE mouse gene expression spatial database

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EMAGE:7774

Apbb2 amyloid beta (A4) precursor protein-binding, family B, member 2 ( MGI:108405)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007659. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007659_01	euxassay_007659_02	euxassay_007659_03	euxassay_007659_04

euxassay_007659_05	euxassay_007659_06	euxassay_007659_07	euxassay_007659_08	euxassay_007659_09

euxassay_007659_10	euxassay_007659_11	euxassay_007659_12	euxassay_007659_13	euxassay_007659_14

euxassay_007659_15	euxassay_007659_16	euxassay_007659_17	euxassay_007659_18	euxassay_007659_19

euxassay_007659_20	euxassay_007659_21	euxassay_007659_22	euxassay_007659_23	euxassay_007659_24

euxassay_007659_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7774_wholemount_strong.wlz

7774_wholemount_moderate.wlz

7774_wholemount_weak.wlz

7774_wholemount_possible.wlz

7774_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7774_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11 14 weak expression: see section 12 13 15

thalamus mantle layer

weak

regional

weak expression: see section 10 11 12 14 15

corpus striatum

moderate

regional

moderate expression: see section 04 05 06 07 18 19 20 21

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 09 10 14 15 weak expression: see section 08 11

midbrain mantle layer

moderate

spotted

moderate expression: see section 09 10 11 12 13 14 15

ventral grey horn

moderate

regional

moderate expression: see section 14 weak expression: see section 11 12 13

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 24

nasal septum

moderate

regional

moderate expression: see section 10 11 weak expression: see section 12 13 14

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35938
Entity Detected:	Apbb2, amyloid beta (A4) precursor protein-binding, family B, member 2 ( MGI:108405)
Sequence:	sense strand is shown >T35938 AGAGAGGGACTTTGCTTACGTGGCGAGAGACAAGGACACAAGGATTCTGAAATGCCATGTGTTTCGATGT GACACACCAGCAAAAGCCATTGCCACAAGTCTCCACGAAATCTGCTCCAAGATTATGGCTGAACGGAAGA ACGCCAAAGCACTGGCCTGCAGCTCCTTACAGGAGAGGACCAATATGAGTCTCGATGTCCCTTTGCAAGT AGATTTTCCAACACCAAAGACGGAGCTGGTGCAGAAGTTCCGCGTGCAGTACCTGGGCATGTTACCTGTA GACAGACCTGTCGGCATGGACACCCTGAACAGTGCCATAGAAAATCTCATGACGTCATCCAGCAAGGAGG ACTGGCCTTCGGTGAACATGAACGTGGCCGACGCCACTGTGACTGTCATCAGTGAAAAGAATGAAGAGGA GGTCTTGGTGGAGTGTCGAGTGCGGTTCCTGTCCTTCATGGGTGTCGGGAAGGATGTCCACACATTCGCC TTCATCATGGACACTGGGAACCAGCGCTTTGAGTGCCATGTGTTCTGGTGTGAGCCTAACGCAGCCAATG TGTCAGAAGCTGTCCAGGCTGCCTGCATGTTGCGGTATCAGAAGTGCTTGGTTGCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64005. Forward Primer - name:064005_F_cDNA_Apbb2, sequence:AGAGAGGGACTTTGCTTACGTG; Reverse Primer - name:064005_N_SP6_cDNA_Apbb2, sequence:TGGCAACCAAGCACTTCTGATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7770 same embryo
	EMAGE:7773 same embryo
	EMAGE:7769 same embryo
	EMAGE:7772 same embryo
	EMAGE:7771 same embryo
	EurExpress:euxassay_007659 same experiment
	MGI:4823171 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source