EMAGE mouse gene expression spatial database

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EMAGE:8852

Gm15800 predicted gene 15800 ( MGI:3647820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010071. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010071_01	euxassay_010071_02	euxassay_010071_03	euxassay_010071_04

euxassay_010071_05	euxassay_010071_06	euxassay_010071_07	euxassay_010071_08	euxassay_010071_09

euxassay_010071_10	euxassay_010071_11	euxassay_010071_12	euxassay_010071_13	euxassay_010071_14

euxassay_010071_15	euxassay_010071_16	euxassay_010071_17	euxassay_010071_18	euxassay_010071_19

euxassay_010071_20	euxassay_010071_21	euxassay_010071_22	euxassay_010071_23	euxassay_010071_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8852_wholemount_strong.wlz

8852_wholemount_moderate.wlz

8852_wholemount_weak.wlz

8852_wholemount_possible.wlz

8852_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8852_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 08 16

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 16

cervical ganglion

weak

regional

weak expression: see section 09 17

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 11 12 13 14 15 16 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32122
Entity Detected:	Gm15800, predicted gene 15800 ( MGI:3647820)
Sequence:	sense strand is shown >T32122 GGAGGTTCATGGCACCAGTGGCTCCTTCCGTCACTTCCTGTGGCAGGTGTGTAAAGAGCTGCAGAGTTCC TCACTGTCGCTGCTCCTGCTGTGTCCCAGCTCTGCAGTCAACAAGAACAAGGGCAAGTACATCCTGACAC CAAGCCCCATCACCTATGGGGAGGAGCAGCTGCTACACTTCTTAGGCCAGCTGCTGGGGATCGCCATTCG TGCAGATGTGCCTCTCCCCCTGGACCTGCTGCCCTCCTTCTGGAAGACGCTGGTTGGCGAGCCTCTCGAC CCTGACCAGGACCTGCAGGAAGCAGACATCCTCACCTACAATTATGTCAAGAAGTTTGAGAGTATCAATG ATGAGAGCGAGCTGGAGGCCTTGTGTGCCGAGATTGCCTCCCAGCACCTGGCCACCGAGAGCCCTGAAGG TCCCAAGCCCTGCTGCCGGTTCACCTACCTGACCATGACTGGCGAGGAGGTGGAGCTATGCAGCCGTGGC CGGCACATCCCTGTGGCTTGGGAGAACAAGGATATCTACGCCGCAGCCATCCGGAGCCTGCGGCTGCGTG AGCTACAGAACATGGAGTGCGTGACAGCCGTGCGTGCTGGCCTGGGCTCCATCATCCCCCTGCAGTTGCT CACCACCCTCAGCCCACTGGAGATGGAATTGCGTACCTGTGGCCTCCCATACATCAACCTGGAGTTCCTG AAGGCCCACACCATGTACCAGGTGGGGCTGATGGAGACAGACCAGCACATCGAGCTTTTCTGGGGGGCAC TGGAGATGTTCACCCAGGAGGAACTGTGCAAGTTCATCAAGTTCGCCTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3985812), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58487. Forward Primer - name:058487_F_IRAV93_f03_AU042671, sequence:GGAGGTTCATGGCACCAG; Reverse Primer - name:058487_R_SP6_IRAV93_f03_AU042671, sequence:TGCAGGCGAACTTGATGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8849 same embryo
	EMAGE:8854 same embryo
	EMAGE:8853 same embryo
	EMAGE:8851 same embryo
	EMAGE:8850 same embryo
	EurExpress:euxassay_010071 same experiment
	MGI:4825088 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source