EMAGE mouse gene expression spatial database

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EMAGE:10249

Uqcrc2 ubiquinol cytochrome c reductase core protein 2 ( MGI:1914253)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018923. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018923_01	euxassay_018923_02	euxassay_018923_03	euxassay_018923_04

euxassay_018923_05	euxassay_018923_06	euxassay_018923_07	euxassay_018923_08	euxassay_018923_09

euxassay_018923_10	euxassay_018923_11	euxassay_018923_12	euxassay_018923_13	euxassay_018923_14

euxassay_018923_15	euxassay_018923_16	euxassay_018923_17	euxassay_018923_18	euxassay_018923_19

euxassay_018923_20	euxassay_018923_21	euxassay_018923_22	euxassay_018923_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10249_wholemount_strong.wlz

10249_wholemount_moderate.wlz

10249_wholemount_weak.wlz

10249_wholemount_possible.wlz

10249_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10249_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 16 17 18 19

pancreas

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 10 11 12

vibrissa

weak

regional

weak expression: see section 07 08 09 21 22

aorta

weak

regional

weak expression: see section 10 11 12 13 14 15 16

orbito-sphenoid

weak

regional

weak expression: see section 03 04 05 06 07 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51170
Entity Detected:	Uqcrc2, ubiquinol cytochrome c reductase core protein 2 ( MGI:1914253)
Sequence:	sense strand is shown >T51170 TTACTACGTCTTGCATCTAGTTTGACTACCAAAGGAGCTTCATCTTTCAAGATAACCCGTGGGATTGAAG CAGTTGGTGGTAAATTAAGTGTGACTGCAACAAGGGAAAACATGGCGTACACTGTAGAAGGCATTCGGAG TGATATTGAGATCCTCATGGAGTTCCTGCTCAATGTCACCACAGCACCCGAGTTTCGCCGTTGGGAAGTA GCTGCCCTGCGGTCTCAGCTAAAGATTGACAAAGCTGTTGCCTTTCAGAATTCACAGACCCGTATCATTG AGAACTTGCATGATGTTGCCTACAAGAATGCCTTGGCTAATCCTTTGTACTGTCCTGACTATAGGATGGG GAAGATAACATCAGAGGAATTACATTACTTTGTTCAGAACCACTTCACAAGTGCAAGAATGGCTCTGGTT GGACTTGGTGTGAGTCATTCTGTCTTAAAGCAGGTGGCTGAACAATTTCTCAACATGAGGGGTGGCCTTG GTTTAGCTGGTGCAAAAGCCAAATACCGTGGAGGTGAAATTAGAGAGCAGAATGGAGACAACCTGGTCCA TGCTGCTATTGTTGCAGAAAGTGCTGCCATTGGAAATGCAGAGGCAAATGCATTCAGTGTACTTCAGCAT CTTCTTGGTGCTGGACCACATATCAAAAGGGGCAACAACACCACCAGCCTCCTGAGCCAGAGTGTTGCCA AAGGAAGTCACCAGCCTTTTGATGTTTCTGCATTTAATGCCAGTTACTCAGACTCTGGGCTCTTTGGAAT TTACACTATTTCCCAGGCTGCAGCTGCTGGAGAGGTTATCAATGCTGCCTACAACCAAGTCAAAGCAGTT GCTCAAGGAAACCTTTCCAGTGCAGATGTCCA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTACTACGTCTTGCATCTAG; Reverse Primer - name:unspecified, sequence:TGGACATCTGCACTGGAAAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10252 same embryo
	EMAGE:10248 same embryo
	EMAGE:10251 same embryo
	EMAGE:10247 same embryo
	EMAGE:10250 same embryo
	EurExpress:euxassay_018923 same experiment
	MGI:4829116 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source