EMAGE mouse gene expression spatial database

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EMAGE:10281

R3hdm2 R3H domain containing 2 ( MGI:1919000)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000306. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000306_01	euxassay_000306_02	euxassay_000306_03	euxassay_000306_04

euxassay_000306_05	euxassay_000306_06	euxassay_000306_07	euxassay_000306_08	euxassay_000306_09

euxassay_000306_10	euxassay_000306_11	euxassay_000306_12	euxassay_000306_13	euxassay_000306_14

euxassay_000306_15	euxassay_000306_16	euxassay_000306_17	euxassay_000306_18	euxassay_000306_19

euxassay_000306_20	euxassay_000306_21	euxassay_000306_22	euxassay_000306_23	euxassay_000306_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10281_wholemount_strong.wlz

10281_wholemount_moderate.wlz

10281_wholemount_weak.wlz

10281_wholemount_possible.wlz

10281_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10281_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 20

trigeminal v ganglion

strong

homogeneous

strong expression: see section 09 18 19 20 moderate expression: see section 08 weak expression: see section 07 21

vagus x ganglion

strong

homogeneous

strong expression: see section 09 19 20 weak expression: see section 21

dorsal root ganglion

strong

homogeneous

strong expression: see section 12 19 moderate expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1710
Entity Detected:	R3hdm2, R3H domain containing 2 ( MGI:1919000)
Sequence:	sense strand is shown >T1710 TGGCCTCGAGCCAGATTCGGACGAGGGGCACGAGGGCCAGTCTGTGCAAGGAGGCCTCCCAACAGGAGGT GTGCCTGTGTACTACAGCATGATCCCACCAGCTCAGCAGAACGGAACGAGCCCTTCTGTGGGCTTTCTGC AGCCTCCTGGCTCTGAGCAGTACCAGATGCCTCAGTCTCCCTCTCCCTGCAGCCCACCACAGATGTCACA GCAGTACTCAGGAGTGTCACCTTCTGGACCAGGTGTGGTGGTCATGCAACTGAATGTCCCTAACGGACCC CAGGCCCCCCAGAATCCATCCATGGTCCAATGGAGTCACTGCAAATACTACAGCGTGGAGCAGCGTGGTC AGAAGCCTGGAGACCTGTACAGCCCTGATGGGAGCCCCCAGGCCAACGCACAAATGGGCAGCAGCCCCGT CACATCTCCTACCCAGTCTCCAGCACCCTCTCCTGTCACGAGCCTCAGCAACGTCTGCACTGGACTCAGC CCCCTGCCTGTCCTCACACCATTCCCCCGGCCTGGGGGTCCTGCACAGGGTGACGGGCGCTATTCCCTA
Notes:	The probe template was PCR amplified from IMAGE:478330 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:478330 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10280 same embryo
	EMAGE:10279 same embryo
	EMAGE:10278 same embryo
	EMAGE:10277 same embryo
	EMAGE:10276 same embryo
	EurExpress:euxassay_000306 same experiment
	MGI:4827561 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source