EMAGE mouse gene expression spatial database

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EMAGE:10731

Gng4 guanine nucleotide binding protein (G protein), gamma 4 ( MGI:102703)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002981. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002981_01	euxassay_002981_02	euxassay_002981_03	euxassay_002981_04

euxassay_002981_05	euxassay_002981_06	euxassay_002981_07	euxassay_002981_08	euxassay_002981_09

euxassay_002981_10	euxassay_002981_11	euxassay_002981_12	euxassay_002981_13	euxassay_002981_14

euxassay_002981_15	euxassay_002981_16	euxassay_002981_17	euxassay_002981_18	euxassay_002981_19

euxassay_002981_20	euxassay_002981_21	euxassay_002981_22	euxassay_002981_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10731_wholemount_strong.wlz

10731_wholemount_moderate.wlz

10731_wholemount_weak.wlz

10731_wholemount_possible.wlz

10731_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10731_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4236
Entity Detected:	Gng4, guanine nucleotide binding protein (G protein), gamma 4 ( MGI:102703)
Sequence:	sense strand is shown >T4236 ACTGAGCCCGGGGCGAGCGATGCCGCTTGGCCTGGCCAACCCCGTCGAGCGCCGCGCCGGGGACCCAGCC CGCGTCTGAGCAGCGCCGGCTGCGCGCCGGCTGCGGAGAACCCCACAGCAGGACCCTGAGTCAGCTTCTC TTCCAACAGCCAACTTCTGCTGCTGCAGTGTCCCCTGACTGGACCTGCCCCTGTACCACCTCTCAGCAGG GAGTGCAGGAATGAAGGAAGGCATGTCTAATAACAGCACCACCAGCATCTCCCAGGCCAGGAAAGCCGTG GAGCAGCTGAAGATGGAAGCCTGCATGGACAGGGTGAAGGTCTCCCAGGCTGCCTCAGACCTCCTGGCCT ACTGTGAAGCCCACGTGCGGGAGGACCCCCTCATCATCCCAGTGCCTGCCTCAGAAAACCCCTTCCGGGA GAAGAAGTTCTTCTGCACCATCCTCTAACACCCATGGCGATGAAGCGGGCCCTTTCCTGCTGTAACAGTT CTGAGTAGAGACCATGCATGCTCCCATGTAGAGGCACACCTCCCACAACACCCCATCTCTGTCCAACCAA TCATGATCTGGGCTCATCTTGTTTTATCATATTTCCTTCATAGCACTGTTTCCTCTTCCTTTTTTTTTTC TTTTTTCTTATTTCCATCCACAGAGAAAACAGACATTTTTATAGCCAAAAAACAAATGTGCCATATAAAA GTGTAGACTTAAAGGTCTACAGTTTTTAAACATTCCCAACCATAAATATATCCAGTTAATAAATTTCAGT GGGTAATTCATTGAAATCAAAACCATGCACAAGTGTCTCCCATGCGCAGTAATTCAATCGCAGATGAGAG CTTGTCAGCCAGACACAGGTGAGGGTGGGGTCTCTTTCGCCTCTGGCATCTCTATCATAGGAGCCTTCAG GGGCCATATGGCTAGGTCCTGTAAATGACTAATATCTCTATCTGTTTATCACTGAACATTCCAACGCTGA TGCTTCTGAGAATACATTTGTGGTGTAGACGCTGGAGCAGCCTTGACTGTAGATACTCACCAGACAGCCC CACTGGGCCATCTTCTCAATCCAAATGCCCCGTTCTCCCTGTTTGCTCCCTCCTTGGTGTTTCTAGTAAG GCAAGAAGTCTGACAACACTGGGTCCTCTGGAGTCTCGGCACAGTTATCCCTGAAAGTGATGCCACCAAT GCAACTCGCTGATCTAAGGATTTATGGGCGTCAACATATTTAACCTAACAATAAGTACTCCAAGTATAGG TCTAGTTTTAGAGAGGGAATATCCCAGGCATAGAGAAATGACATTTGCTGAAAACTGCAGATAGAAAGTA AGCACACCAAGACGTCCATGTGTGGGTAGTCTATCCCTAAAACCCTCCCTGAAGCTCTGAACTGTGGACA CCTGTGGCAAGCGTGCAGCTTCACAAACAGTCCTAACTGCACGCTTGGAATGAAGCAAACACATTTTCAC ACTAACTGGATGTACACCTCTCTCCCACCTTGGCCAAGAGAAGTGTCACAAGCCTAAGTTATGGCAGTTT GTAATAGATGATATGTAGGTGCCCCAAGATCAGATAGCAGTAAGCCTTGAGATTTTCCATGACAAGGCTG TTGGCCCCATCTAAGGCACCCCCATACCCTCCCAATGGAGGCGGTAACCACAGTTGCTGAGGGCTCTGTG AGAACATTTGTTGTGTAGATATTTGAGAACTACCCCTCCTCTGGATCTCAACGAGGCTTGCTGTGTGCTG CTGGGCAAGCCGTTGTGTGCCAAGCTGTTCAAACCGGCAAACTGCAGACAGTTAATTACTCCTCTTGCTT TTCTGCCAGGGCAGGAAACCAAGCAAAGCTCGCATTATCCTTGAAAGCCAACATGTGCTGGATGACAGCC TATAATGCTGTGGAGAGTATATGATTTCACAGACACCATGGTTCTGAGACGAGGAGTGCAAAATGGCCTC CAAGGAGGTGACTATCATTTTTAGATGGGTTTGTGCCTTGCTACCCTCTCTTGCAGCTTTGGTGGAAGCT GTTCTTAGTTTCACTACTGGGCACGAGATGAGAGGCTAAGACTCTGGTTTTGTTGGGCTGTAAGAAGATC TCTTTATTCTGTGGCTCATGTTGGCATCATCAACCGACAGAGTAGACCATAGTCCACAAAAGTCAAGAAG TGGGGCAGCACCAGCAGTAGCAAAATCAGGTCAAGAGAACAGTGTGCTTTAATCTCCTACCAAACTGTCT CTGCTTTCCTGCCTCTCCTTCCCACACATCTTCCTTACATTCTCCTGTGGATGCCGTGTCCACTAGTAAC TGTCCTGGGAAGATTCCCCTCCCTCACCCCCACCCCTGCCACTCTACCACTTGGACCCATTGAGCTTTCT GACTTATTCCTGAATTTCTGTTAATTCTCAGAGTCTTGTGATCAACCTTGTAAACTCATTATTCCACAAA GTCTCCTCATTTGGATGTGAATATTTTGTGAGGAGACAACTCTGCCAGAGTGTGTACTCAACAGAAATGT GTACATATCATCAGAAGAAATGCACTAGAGTGTTAGAGAAGTACTGCTTACAGTAACCATAGCCAGAAGG AGCCCCTTACACCCATGCCAAAGGAATAAACAGATGAGGGTAATTTGTGCAGTGGATTCTCAGCTCTGTC CACAGCTTGGTGTGAATCTTGCTGGCTTGAGGGACTAAGAGCAGGCAAGTCCAAGCTACAGTTCCATTTC CATACAGCACAGGCCTGGCAGGCCAACCCAAGCAGATTAAAGGAGGGACAGTAGCTATTATTCTCAAAGG GGCCACAGAGAGGGGACACAAAAGGCTTCTGGAATGTTCTGTCTCTGGATCTAGCTGTAGGTCATCCTGA TGTGTTTAGTTTGTGCAAGTTCTCCAACTGTCCACTTTTGGGTGCTTTTCTGTATGTGTGTTATACTTCA ATAAAACATCAGGCTTTGTGATTACCAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502719 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502719 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10732 same embryo
	EMAGE:10730 same embryo
	EMAGE:10729 same embryo
	EMAGE:10728 same embryo
	EurExpress:euxassay_002981 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source