EMAGE mouse gene expression spatial database

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EMAGE:10732

Pla2g5 phospholipase A2, group V ( MGI:101899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001141. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001141_01	euxassay_001141_02	euxassay_001141_03	euxassay_001141_04

euxassay_001141_05	euxassay_001141_06	euxassay_001141_07	euxassay_001141_08	euxassay_001141_09

euxassay_001141_10	euxassay_001141_11	euxassay_001141_12	euxassay_001141_13	euxassay_001141_14

euxassay_001141_15	euxassay_001141_16	euxassay_001141_17	euxassay_001141_18	euxassay_001141_19

euxassay_001141_20	euxassay_001141_21	euxassay_001141_22	euxassay_001141_23	euxassay_001141_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10732_wholemount_strong.wlz

10732_wholemount_moderate.wlz

10732_wholemount_weak.wlz

10732_wholemount_possible.wlz

10732_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10732_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

weak

regional

weak expression: see section 10 11 12 17

hypothalamus mantle layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

hypothalamus ventricular layer

weak

regional

weak expression: see section 11 12 13 14 15 16

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 11 12 13 14 15 16

telencephalon marginal layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 11 12 13 14 16 17 18 19

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14 16 17 18 19

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

weak

single cell

weak expression: see section 09 10 11 12 13 15 16 17 18 19

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17

metencephalon basal plate

moderate

regional

moderate expression: see section 08 19 21

pons mantle layer

weak

single cell

weak expression: see section 09 10 11 12 15 16 17 18 19 20 21

pons ventricular layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22

midbrain mantle layer

weak

single cell

weak expression: see section 07 08 09 10 11 12 13 17 18 19 20

midbrain marginal layer

weak

single cell

weak expression: see section 12 13 14 15 16 17

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 moderate expression: see section 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 21

ventral grey horn

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 16 17

intermediate grey horn

moderate

regional

moderate expression: see section 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 12 17 18 19

cervical ganglion

weak

regional

weak expression: see section 19

thoracic ganglion

weak

regional

weak expression: see section 13 14 17

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 20

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

testis

moderate

regional

moderate expression: see section 06 07 08 09 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4234
Entity Detected:	Pla2g5, phospholipase A2, group V ( MGI:101899)
Sequence:	sense strand is shown >T4234 ACTTTCTGCCACCATGAAGGCTGCTGGGGGCTGAGCGGGATGCTTGCTGGCCAGAGTTCATTGCGTGCTC CCATGATCAGAGTCTGTCCTCCTGTGTTGCAGCTTGTAGACCCTGATGCTTGTGCTGCCGGCCATAAACG CAGGGATCCAGACTCTGTCTACTGAGGCTGCTCCTAGGAACGGGCCCTGGGAACAGGCGCTGAGACCAGG CTACAAAGAACCCAAGAGATGAAGGGTCTCCTCACACTGGCTTGGTTCCTGGCTTGCAGTGTGCCTGCAG TCCCAGGGGGCTTGCTAGAACTCAAGTCCATGATTGAGAAGGTGACCAGGAAGAATGCCTTTAAGAACTA TGGCTTCTACGGCTGCTACTGTGGCTGGGGCGGTCGCGGGACACCCAAGGATGGCACTGATTGGTGCTGT CAGATGCACGACCGTTGTTATGGGCAACTGGAGGAAAAAGACTGTGCCATTCGGACCCAGTCCTATGACT ACAGATACACAAATGGCCTAGTCATCTGCGAACACGACTCCTTCTGTCCAATGAGGCTCTGTGCCTGTGA CCGGAAGCTGGTCTACTGCCTGCGGAGAAACCTCTGGACTTACAACCCTCTTTACCAGTATTACCCCAAC TTCCTCTGCTAATGTCCGCTGTGCGCTCTGCCCTGGGGGCACCTCCCACGGTGGCAGTCTCCCTCTGCTG TATTCCTGATGTGTCCCGCCTCCCCCACAAGGTCTTGGATCTGCCTTCCTCTGTGCACCAGTGGACTGGA CAGAGCCCCCCCCCCCCCCCAGGGCTACACTCTTCCCTCCAGAGTCCGAGGAAGGGACTTTGATCTAGAG TTGGCAGACTCTCAGGATGGCTGAGCCTGCACTTGCTAAGTTGGCTCTGGGCCCTGAAGCTCCCCCGGCT CAGGCCAGCCGTGTTGACTTTTCTTTCAATTTCTGGAACCCAACTGTCATTAGCACCCTCCAGAGACCTT TTACTGGAGGAGAAGCCAAATTAACTCTATAAATCTGCTATGTAGATATTAAATAAAACCCATTCTCAAG GCTAGAAAAACACCACCCCAGCACTTCCTCTGACGGGGCTGGGGTGGGATTGCCCACGCTTTTCCAACCC CCCCCCCCCCCCGAGGCATCTGTATGCTCTCGGGGATGGAGGTCAGGAAACAGGTGGGAGCCTTGACGCC TTGATGCCTTTCATGGTTTGTCTTGAGTTCATTTTCTTCAACCTTAGGCTCCTTCAAGCCAGACCTGGGG TTTATGTTTCTTCTGGAGGAAGATGAGACACAGCCCTATGCCACCTTGAGCTCCTGGCTAGAGAGGGCAA CAATCAGCTCCCAGCCCTGGCTTCTGGCAGCTGTATGGTCTTTTAAACCTTTGGATAGTCCGAATCTCTC TGGCTCTCCTCAAAATATAAAACAAGGCTCCTTCCAGGAGTATACTGGTGCACACCCCAATCCCAGCACC TGGAAGGTGGAGGCAAGAGAATCGGGAGTTCAAGGCCAGCCTCTGCTCCCTAGCAGGGATAGTAGCCTAG ACTATGTGAGACTGTGTTTCAGAAAGGACCCTGGTACAGATACAGGGGAGCTGGGGTTCTGAGATGTCAC TCAGTGAGAAAAATGGTTTAAATAAGATTCGGGGGGGGGGGGTAGAGATATATCAATGGCGGAATGTCTG TCTATTTTTCACCGAGTTAGGGGCTCTGAAAATCTCAAGTCATCTATCGCTTTATAAAAGACAGAACTTT CTCATAGCAACAAGACAGGAGGAAGAGATGTATTCAACAGTAAAGCAGTGTCCTTCCGGAGGAAGGGTCT AGGCTTCTTCAAATGGAGCTCCAGAATGTGCACGGGACACATCCGATCTATATTTAGAGACCTTTAGTGT TCAAACATTTTCTAAATAAAGCTAATTATATGAGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502326 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502326 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10731 same embryo
	EMAGE:10730 same embryo
	EMAGE:10729 same embryo
	EMAGE:10728 same embryo
	EurExpress:euxassay_001141 same experiment
	MGI:4827243 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source