EMAGE mouse gene expression spatial database

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EMAGE:11009

Nacc2 nucleus accumbens associated 2, BEN and BTB (POZ) domain containing ( MGI:1915241)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001103. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001103_01	euxassay_001103_02	euxassay_001103_03	euxassay_001103_04

euxassay_001103_05	euxassay_001103_06	euxassay_001103_07	euxassay_001103_08	euxassay_001103_09

euxassay_001103_10	euxassay_001103_11	euxassay_001103_12	euxassay_001103_13	euxassay_001103_14

euxassay_001103_15	euxassay_001103_16	euxassay_001103_17	euxassay_001103_18	euxassay_001103_19

euxassay_001103_20	euxassay_001103_21	euxassay_001103_22	euxassay_001103_23	euxassay_001103_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11009_wholemount_strong.wlz

11009_wholemount_moderate.wlz

11009_wholemount_weak.wlz

11009_wholemount_possible.wlz

11009_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11009_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 22 23 24

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 17 18 19 20

cerebral cortex marginal layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate

weak

homogeneous

weak expression: see section 08 09 16 17

lower jaw incisor

weak

regional

weak expression: see section 13 14 17 18

lower jaw molar

weak

regional

weak expression: see section 20

upper jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

upper jaw molar

weak

regional

weak expression: see section 20

kidney calyx

weak

homogeneous

weak expression: see section 06 07 08 09 10 17 18 19 20 21

tail

weak

regional

weak expression: see section 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4854
Entity Detected:	Nacc2, nucleus accumbens associated 2, BEN and BTB (POZ) domain containing ( MGI:1915241)
Sequence:	sense strand is shown >T4854 CGGACGCGTGGGGTCCGCGGGCGCCGGAGCTCGGGGACGCCGGAGCACTTCCTGCCGCGGGCGGCGCCGC TGCTGCCCAGGCCGGACTGGGCCGGGTGCCCGCAGATGTGCCTCATCCAGGCTGCCCCACGTTGAGTCTG AGACCCACCCACCACAGCACCCACCATGTCTCAGATGCTGCACATCGAGATCCCCAACTTCGGGAACACA GTGCTTGGCTGTCTCAACGAACAGCGCCTGTTAGGCCTCTACTGTGATGTGTCCATCGTGGTCAAAGGCC AGGCCTTCAAGGCCCACCGTGCCGTCTTGGCCGCCAGCAGCCTCTATTTCCGAGACCTCTTCAGTGGTAA TAGCAAGAGTGCATTCGAGCTGCCGGGCACGGTGCCGCCTGCCTGCTTCCAGCAGATTCTGTCCTTCTGC TACACAGGTAAGCTTACCATGGCGGCCAGCGAGCAGCTCGTGGTCATGTACACAGCCGGCTTCCTGCAGA TCCAGCACATCGTGGAGCGGGGCACAGATCTCATGTTCAAGGTGAGCTCACCCCACTGTGACTCGCAGAC CGCCATGATCGAAGATGCCAGCTCGGAGCCCCAAAGTCCCTGCAACCAGCTGCAACCGGCCACTGCTGCC TATGTCACGTCTCCTTCTGTGCCCATCCCGCTTCTGACCCGTGTAAAGCATGAAGCTATGGAGATGCCGC CGGCCAGTGGCCCGGGCCTGGCTTCTAAACGCCCACTAGAGACGGGCCCTCGAGATGGTGTGGCAGTAGC TACAGGTGCTGCAGGGACACCTGGCACGGCCCCTCTGAAGCTGCCTCGAGTCTCCTACTATGGTGTGCCA AGCCTGGCCACTCTCATCCCCAGCATCCAGCAGGTACCCTATCCCCCGGGGGAGCGGACCAGTCCTGGGG CCAGCAGCCTGCCCACCACTGACAGCCCCACCTCCTACCACAACGAGGAGGATGAAGAGGATGACGAAGC CTATGATACCATGGTGGAGGAGCAGTATGGCCAGATGTATATTAAAGCCACAGGAAACTATGCAGTGCAA GAGAAACCAGAGCCAGTGCCCCTGGAGAGCCGCTCCTGTGTCCTCATCCGTCGAGACCTGGTGGCACTTC CTGCCAGCCTCATCAGCCAGATTGGGTACCGCTGCCACCCAAAGCTCTACTCAGAGGGAGATCCTGGAGA GAAACTGGAGCTTGTAGCAGGCTCCGGGGTCTACATCACACGTGGCCAGCTCATGAACTGCCACCTGTGT GCAGGGGTGAAGCACAAGGTCCTGCTGCGGAGGCTCCTGGCCACCTTCTTTGACAGGAACACACTGGCCA ACAGCTGTGGCACTGGCATTCGCTCCTCCACCAGTGATCCGAGCCGAAAGCCACTGGACAGCCGAGTCCT GAACGCTGTGAAACTGTACTGTCAGAACTTTGCCCCCAGCTTCAAAGAGAGCGAGATGAACGTGATTGCC GCAGACATGTGTACCAATGCTCGCCGTGTCCGTAAGCGCTGGCTGCCCAAGATCAAGTCCATGCTTCCAG AGGGTGTGGAGATGTACCGTAGTGTCATGGGTGCGTCGGCCGCCAGCCTGCCTCTGGACCCCGAGTTCCC ATCTGCGGCTCCACAGGTGTTTGAGCAGCGCATCTATGCTGAGCGACGAAGTGATGCAACCACCATCGTG GCTCTGAGAACTGATGCGGTGAATGTCGATCTGAGCACTTCAGCCAACCCTGCCTTTGAGGCTAATGAGG AGGTGGATGGGGGTGGCTCTGTTATCCAGGAGGTGGCTGCCCCAGAGCAGCTGCCCGCTGATGGCCAGAG CTCCCCTCAGGCCTTTGAGCAGGGCAACACCAGCTCCAGTAGGCCTCAGACACCAGTGGCCACAGCAACA AGAAGGCCAGAGGGCACCTATGCAGGGACCTTGTAAGTGGCCGGCAAGGCCAGCTCACAGGAACCCAGTA CTAAAGCTGCTTGCATGCGTTACTAATACAAACAAATGTGATCAAGCCACTTACCTACTGAACTGCTACT GCTGCCTGACAAAAATGTGATTTTTATTCTGCCTGTATTTAAAATGGATGAAGGAAACAAATGCATTCAT TATACTGTAAACATTTAGGCCGCTGGCCACTCTCTGGGACAAAGGTCCCAGGGCAACTTTTTGATATTCC CCAGCTCAGTCTCCCGTGGTAGTGTTCTCTCTCCCTTCTTGGGGAGACAGAAGAGGGCACCGGGTCCCTG ATGTCCTGCCCTGACCCTGCCTCATCCACCCTTCTTATTCCCCTGCTCTGAAAGCAGAAGCTGGGGCAGC AGTGGCCTGGGGCTGGAGGGGCAGACACGACACTCCATTCTTACCCATAAGGACCCCACTGGGACAGTGG GGGGAATGCTGGGCCCTCAGAGGCCAGACTTCTCTTGTTTTCATTCCTCCGAAGAAGCTGCCTGCGTGCA TGTATGTATGCGTGTGTGTGTGTGTGTGCGCGTGTGTGTGTGTGTGTGTGTGTGTGTGCATGTGTGTGTA TGAGTATGTACGCACACACACATGAGTGAGAGAGACAGAGAGAGAGAAAGAGAAAGAGGGGGGGCAGGAA GACCAGTGATGGCCCTGCAGTAGCTGTGGCTGGTCATCTGGGGGTGGGGGAAAGATGAGGTGTTCCCCCC TCCATACCTGTGTCCTCACTCTGCTCCACCCAGACCCCCAGCGGCTGGCCTGCTGATCCCCCCAGGGTCT CTCTGCCCCTGGCCATGGCTGTCCTCACCACCCTCTGCCTCTCCAGCCACTCTGGGATTTTGCTTGTTTG CTGTTTTTGTTTAGTTCAGATCTATTTTGTTTGTGGTTTGGAAACTTTCAGACCGAACAGAGAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5067063 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067063 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11008 same embryo
	EMAGE:11012 same embryo
	EMAGE:11011 same embryo
	EMAGE:11010 same embryo
	EMAGE:11007 same embryo
	EurExpress:euxassay_001103 same experiment
	MGI:4826586 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source