EMAGE mouse gene expression spatial database

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EMAGE:11057

Ergic2 ERGIC and golgi 2 ( MGI:1914706)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003183. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003183_01	euxassay_003183_02	euxassay_003183_03	euxassay_003183_04

euxassay_003183_05	euxassay_003183_06	euxassay_003183_07	euxassay_003183_08	euxassay_003183_09

euxassay_003183_10	euxassay_003183_11	euxassay_003183_12	euxassay_003183_13	euxassay_003183_14

euxassay_003183_15	euxassay_003183_16	euxassay_003183_17	euxassay_003183_18	euxassay_003183_19

euxassay_003183_20	euxassay_003183_21	euxassay_003183_22	euxassay_003183_23	euxassay_003183_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11057_wholemount_strong.wlz

11057_wholemount_moderate.wlz

11057_wholemount_weak.wlz

11057_wholemount_possible.wlz

11057_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11057_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4700
Entity Detected:	Ergic2, ERGIC and golgi 2 ( MGI:1914706)
Sequence:	sense strand is shown >T4700 TGGGACCGTACCACTAGTATGACTATATGAAGGAGAAGGAAGGTTTTTCTGAAGATGAGGCGTCTGAATC GGAGAAAAACCTTAAGTTTGGTGAAAGAGTTAGATGCCTTTCCCAAGGTTCCTGACAGCTATGTGGAGAC GTCAGCCAGTGGGGGAACAGTTTCTCTAATAGCATTTACCACTATGGCTTTATTAACAATAATGGAATTC TCAGTCTATCAAGATACATGGATGAAGTACGAATATGAAGTAGACAAGGATTTTTCTAGCAAGTTGCGAA TCAACATAGACATTACTGTTGCCATGAAGTGTCACTATGTTGGAGCAGATGTACTGGATTTAGCAGAGAC CATGGTTGCTTCTGCAGATGGTCTGGCTTATGAACCAGCACTGTTTGACCTTTCACCCCAGCAGAGAGAG TGGCAGAGGATGCTGCAGCTGATTCAGAGTAGGCTGCAGGAAGAGCATTCCCTTCAAGACGTGATATTCA AGAGTGCTTTTAAAAGTGCATCAACTGCACTGCCACCAAGGGAAGATGATTCATCACTGACTCCAGATGC GTGCAGAATCCATGGTCATCTCTATGTCAATAAAGTAGCAGGGAATTTTCACATAACTGTGGGCAAGGCA ATCCCACATCCTCGAGGGCATGCACATTTGGCAGCACTTGTCAACCATGATTCTTACAACTTTTCTCACA GAATAGATCATTTGTCTTTTGGAGAGCTTGTTCCGGGAATTATTAATCCTTTGGATGGAACTGAAAAGAT TGCTGTAGATCACAACCAGATGTTCCAATATTTCATTACAGTTGTGCCAACAAAGCTACACACATACAAG ATCTCAGCAGATACCCATCAGTTCTCTGTGACAGAAAGGGAACGCATCATCAACCATGCTGCAGGCAGCC ACGGCGTCTCTGGAATATTTATGAAATATGATCTCAGTTCTCTCATGGTGACAGTCACTGAGGAACACAT GCCTTTCTGGCAGTTTTTTGTAAGACTCTGTGGTATAATTGGAGGAATCTTTTCAACAACAGGCATGTTA CATGGAATTGGAAAATTTATAGTGGAAATAATTTGCTGTCGTTTCAGACTTGGATCCTACAAGCCTGTCC GTTCTGTCCCCTTTGCGGATGGCCACACAGACAACCACTTACCTCTTTTAGAGAACAACACACATTAGTA CCTTGTGGGTGGAGAAAAACTTTTTGCCTGAGACATAAAGCCTTTTCTAATAATAACATGTTGTGCAATA TATTCAAAGAAAAAGAAAATATGAGAACCCGGAAACATATTTAAAAAAGAAAAAAACAGCCAAGGAAAAC AACAAACTGCAGTCGTCTTGTATATGTTGTGTCTGTTACAAATGTCATAGAAGAAATTATGCAGCTAACT GGAGAGTCAGCCCAGCCAACAGTGCCAGGTGAAGACAGCACCTCTGATGCCTCCCAGCAAACAGACAATA TGGAAAGCAGATCCTAGAGGATGGACGTCAACCCGAGTTTAAAGAAAAGGGCATTGATAAATGCAAATGT GTGCGTCCTTTGTTTTTAAAGATGTCAGAATAAAATGTGTAAAACATATGGAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030661 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030661 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11055 same embryo
	EMAGE:11058 same embryo
	EMAGE:11059 same embryo
	EMAGE:11054 same embryo
	EMAGE:11056 same embryo
	EurExpress:euxassay_003183 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source