EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11058

Liph lipase, member H ( MGI:2388029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001274. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001274_01	euxassay_001274_02	euxassay_001274_03	euxassay_001274_04

euxassay_001274_05	euxassay_001274_06	euxassay_001274_07	euxassay_001274_08	euxassay_001274_09

euxassay_001274_10	euxassay_001274_11	euxassay_001274_12	euxassay_001274_13	euxassay_001274_14

euxassay_001274_15	euxassay_001274_16	euxassay_001274_17	euxassay_001274_18	euxassay_001274_19

euxassay_001274_20	euxassay_001274_21	euxassay_001274_22	euxassay_001274_23	euxassay_001274_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11058_wholemount_strong.wlz

11058_wholemount_moderate.wlz

11058_wholemount_weak.wlz

11058_wholemount_possible.wlz

11058_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11058_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

anal canal

weak

regional

weak expression: see section 16

stomach fundus mucosa

weak

regional

weak expression: see section 05 06 07 08 09 10 11

bladder

moderate

regional

moderate expression: see section 14 15 16

testis

moderate

regional

moderate expression: see section 07 08 09 10 20 21 22

duodenum caudal part lumen

weak

regional

weak expression: see section 12 15 16 17 18

midgut loop lumen

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

jejunum lumen

weak

regional

weak expression: see section 10 11 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4188
Entity Detected:	Liph, lipase, member H ( MGI:2388029)
Sequence:	sense strand is shown >T4188 CCACGCGTCCGCGGGAACGCGGGAACGCCAGGCTCTGTGCGGAGTGCGACACGTCCTCGTGTTTGGGGCA CATGATCTCTTGAGGTTCCCTATGCTGAGGCTATGTTTTTTCATCAGTTTCATGTGCTTGGTGAAATCAG ACACGGATGAAACATGCCCGTCCTTCACCAGACTGAGCTTCCACAGCGCGGTGGTTGGTACAGGACTCTC CGTGAGACTGATGCTCTACACACAGAGAGACCAGACCTGTGCACAGATCATCAACTCCACAGCTCTGGGG AGCTTAAATGTGACCAAGAAAACCACTTTTATAATCCACGGTTTCCGGCCAACAGGCTCCCCTCCGGTTT GGATAGAGGAGCTGGTACAGAGTTTGATCAGTGTACAAGAGATGAATGTAGTGGTTGTTGATTGGAATCG AGGAGCGACAACTGTGATATACCCCCATGCGTCTAGCAAGACCAGACAAGTAGCTTCTATTTTGAAGGAA TTTATTGACCAGATGTTGGTCAAAGGAGCTTCTCTGGACAACATTTACATGATTGGAGTAAGTCTAGGAG CCCACATAGCTGGATTTGTTGGCGAGTCGTATGAGGGGAAGCTGGGGAGAGTCACAGGTCTTGACCCTGC AGGCCCTTTATTCAACGGGAGACCTCCTGAGGAGAGATTAGACCCCAGCGACGCACTGTTCGTGGACGTC ATCCACTCTGACACTGACGGTATAAAGTACTTCAAGTGTGACCACCAGATGTCCGTCTACCTGTACCTTG CATCCCTGCAAAATAACTGCTCCATCACGGCCTATCCCTGCGACTCCTATCGGGATTATAGGAATGGCAA GTGTGTCAGCTGTGGCGCTGGACAAATTGTGCCATGTCCCCGTGTAGGCTACTATGCTGACAGCTGGAAA GAGTACTTATGGGACAGAGATCCTCCGATGACGAAGGCGTTCTTCGACACAGCTGAGACAAAGCCATACT GCATGTATCATTATTTTGTGGACATTGTATCTTGGAACAAGAGCGTAAGAAGAGGGTTTATTACAATCAA ACTGAGAGGCGAAGATGGCAATATCACAGAATCCAAAATCGATCATGAGCCATCTGCATTTGAGAAATAC CATCAAGTGAGTCTCCTCGCAAGATTTAATCGAGATCTGGATAAGGTGGCAGAGATTTCCTTGTTGTTCT CCACAGGGTCTGTAGTGGGCCCAAAGTACAAGCTCAGGGTCCTGCAGATGAAGCTGAGGTCTCTGGCCCA TCCAGACAGGCCTCACTTGTGTCGATATGATCTCGTCCTTATGGAGAATGTTGAGACATCCTTCCAACCT ATCCTGTGCTCTCAGCAGCAGATGTAGCTGCTGTTAAGACCTGTGGGCAGTGATGACATCAAGATCTCTA CAGCTATAGGCTTTCAGTGCCTCACCCTATAGCGTCCTGAAGGCTTCCAAAGCATGGGGAAGAAAACCAA GGTTATTTTCGGCTGTGTTCCAAGGATGTCGCGTCACAAAAATGTCAAAGGACCTGCGATTATGAAACAC CACTGGGAGGGGGGGGGCGCCCTAACTAGGGCAAGACAGAAATAGCCCAGCTCCCTACAGCAAAGCACTT TGTCTGGCTGCGTCCCGAGGCCCCACTTGTTCCCCACTGTCGAATCTGCTGCCTGTCATACCACACCCTC AGGGCCGTGGGGCAAGCATCTAAATGGTCCCTGTGCCCAATCCTCTGCCATCTCGGCGGGAACCGAATGG AGAATGAACCAGACTACCTCACAGGGACTGTGGGTGGGGAGGGCCAGGCTGTGAAGGGTTTGGAAATCCC TTGGGTCCCTTGGGTGGACTACTGTGAACATGCTTTTCTTTTGATAGGGTTTGGCTCTTACGTAAAGACA CACAATCTCTCTTTTTCAACACCAGCTTTCCTCCTGGACTGTTTGCCTCGGGGCGACAAGAAGGCTCTGG CTTAGTCCAGACTCTTCTGAAGTGATGCTGATGGCCAGAGGTATTGGGCTGGGCTCATAGCCCTGAGGCC TGTGCAGCTAAGTGGAAGAAAGAGAAAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487939 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487939 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11055 same embryo
	EMAGE:11057 same embryo
	EMAGE:11059 same embryo
	EMAGE:11054 same embryo
	EMAGE:11056 same embryo
	EurExpress:euxassay_001274 same experiment
	MGI:4825938 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source