EMAGE mouse gene expression spatial database

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EMAGE:11063

Abcd2 ATP-binding cassette, sub-family D (ALD), member 2 ( MGI:1349467)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001188. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001188_01	euxassay_001188_02	euxassay_001188_03	euxassay_001188_04

euxassay_001188_05	euxassay_001188_06	euxassay_001188_07	euxassay_001188_08	euxassay_001188_09

euxassay_001188_10	euxassay_001188_11	euxassay_001188_12	euxassay_001188_13	euxassay_001188_14

euxassay_001188_15	euxassay_001188_16	euxassay_001188_17	euxassay_001188_18	euxassay_001188_19

euxassay_001188_20	euxassay_001188_21	euxassay_001188_22	euxassay_001188_23	euxassay_001188_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11063_wholemount_strong.wlz

11063_wholemount_moderate.wlz

11063_wholemount_weak.wlz

11063_wholemount_possible.wlz

11063_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11063_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16 17

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 13 14 18 19 20

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 moderate expression: see section 01 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 03 04 05 17 18 19 20 21 22 23 weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 17 18 19 20 21 22 23 weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

pons ventricular layer

moderate

regional

moderate expression: see section 17 18 19 20 weak expression: see section 06 07 08 09 10 11 12 14 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 17 18 19 20 21 moderate expression: see section 22

tongue

weak

regional

weak expression: see section 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4677
Entity Detected:	Abcd2, ATP-binding cassette, sub-family D (ALD), member 2 ( MGI:1349467)
Sequence:	sense strand is shown >T4677 CCACGCGTCCGTAAGAGTAAAGGAGACAAGCTGCAGAGCATGGGAGGCTGTGGGGTCTTCTGAAACCTTT GCTGGGCTTTCCGCGGAGCATGAGCTTTTAAAACGAATTCTTTTCAAAGAAACCCATTTGTGTAGCTGGA AAAATGATACACATGCTAAATGCAGCAGCCTATCGGGTGAAATGGACCAGATCCGGTGCTGCTAAAAGGG CTGCCTGCCTGGTGGCTGCGGCATATGCTCTGAAAACCCTCTATCCCATCATTGGCAAGCGTTTAAAGCA GCCTGGCCACAGGAAGGCAAAAGCAGAAGCTTACTCGCCTGCAGAGAACAGAGAAATACTGCATTGCACG GAGATCATCTGTAAAAAACCTGCGCCGGGACTAAATGCAGCTTTTTTCAAACAGCTACTAGAACTTCGGA AAATCCTCTTTCCAAAACTTGTGACCACTGAAACGGGGTGGCTCTGCCTCCACTCGGTGGCTCTAATCTC AAGAACATTTCTCTCTATTTATGTGGCTGGTCTGGATGGGAAAATCGTGAAAAGCATCGTGGAAAAGAAG CCTCGGACTTTCATCATCAAATTAATCAAGTGGCTTATGATTGCTATCCCTGCTACCTTTGTCAACAGTG CTATCAGGTACCTGGAATGCAAACTGGCATTGGCCTTTAGAACTCGCTTAGTAGACCATGCCTATGAGAC CTATTTCGCAAATCAGACTTATTATAAGGTGATAAATATGGATGGGAGGCTGGCAAACCCTGACCAGTCT CTTACCGAAGACATTATGATGTTCTCGCAATCTGTGGCTCACCTGTATTCCAACCTTACCAAACCTATTT TAGATGTCATTCTAACCTCCTATACTCTCATCCGGACAGCTACATCCAGAGGAGCAAGCCCTATAGGGCC CACCCTGTTAGCAGGACTTGTCGTGTATGCCACTGCTAAAGTACTGAAAGCTTGCTCGCCCAAATTTGGT TCGCTGGTGGCTGAAGAAGCCCACAGGAAAGGCTACCTGCGGTATGTCCACTCCCGAATCATAGCCAATG TAGAAGAAATTGCCTTCTACAGAGGACATAAGGTAGAAATGAAGCAGCTGCAGAAATGTTACAAGGCTTT AGCTTACCAGATGAACCTGATTTTATCCAAACGTTTATGGTACATCATGATAGAACAATTCTTGATGAAG TATGTGTGGAGCAGCTGTGGACTAATTATGGTGGCTATACCCATTATCACTGCAACGGGCTTTGCAGATG GTGATCTGGAGGATGGTCCAAAGCAGGCTATGGTTAGCGATCGGACAGAGGCCTTCACCACTGCCCGGAA CTTACTGGCCTCTGGAGCTGATGCAATTGAAAGGATTATGTCTTCATACAAAGAGATCACTGAACTAGCA GGTTATACTGCTAGAGTATACAATATGTTCTGGGTCTTCGATGAAGTGAAGAGAGGCATTTATAAGAGAA CTGTCACTCAGGAACCTGAAAACCATAGCAAGCGTGGAGGTAACCTGGAACTACCCCTCAGCGACACCCT GGCCATCAAAGGAACAGTTATTGATGTGGATCATGGAATCATTTGTGAAAATGTTCCCATAATTACACCA GCGGGCGAAGTGGTGGCTTCCAGGCTAAACTTCAAAGTGGAAGAAGGGATGCATCTCTTGATAACTGGTC CCAACGGTTGTGGGAAAAGCTCTCTCTTCAGAATCTTAAGCGGGCTGTGGCCTGTGTATGAAGGAGTCCT TTATAAACCGCCTCCCCAACATATGTTCTATATTCCACAGAGGCCATACATGTCTCTTGGAAGTCTCCGG GATCAAGTCATTTACCCTGACTCAGCGGATGACATGCGTGAGAAAGGTTACACTGACCAAGACCTAGAAC GCATCCTGCACAGCGTGCACCTCTACCACATAGTTCAAAGAGAAGGAGGATGGGATGCAGTCATGGACTG GAAAGATGTCCTTTCCGGAGGGGAGAAGCAGAGAATGGGCATGGCGCGGATGTTTTACCATAAACCGAAG TATGCATTGCTGGATGAATGTACCAGTGCCGTGAGCATCGACGTTGAAGGAAAGATATTTCAGGCTGCTA TTGGGGCTGGGATTTCCCTACTCTCCATAACACACAGGCCTTCTCTGTGGAAATACCACACTCATCTATT ACAATTCGATGGCGAAGGAGGCTGGCGCTTTGAACAGTTGGACACTGCTATCCGTTTAACGTTGAGTGAG GAAAAGCAAAAGTTGGAGTCGCAGCTCGCTGGAATTCCCAAAATGCAACAGAGACTCAACGAACTATGCA AAATTCTGGGGGAAGACTCGGTGCTGAAAACAATCCAAACTCCAGAAAAGACATCCTAATTTATCTTGAC ATGTTTTCAGTTACCTTCTAGGTGAAGCCTCAGAGACTCTCTCTTTACTGCATGCAGTATGTTAAGCTAA GTGCAGAGAAAGCAAGCCGGCAAAAAAAAAGTATTTTGTATGATTTTAGCATCGAGGAAATATATGAAGA CTTTTCAAACAAACAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5027149 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5027149 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11067 same embryo
	EMAGE:11062 same embryo
	EMAGE:11066 same embryo
	EMAGE:11061 same embryo
	EMAGE:11064 same embryo
	EMAGE:11065 same embryo
	EurExpress:euxassay_001188 same experiment
	MGI:4822866 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source