EMAGE mouse gene expression spatial database

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EMAGE:11096

Rasgef1b RasGEF domain family, member 1B ( MGI:2443755)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003547_01	euxassay_003547_02	euxassay_003547_03	euxassay_003547_04	euxassay_003547_05

euxassay_003547_06	euxassay_003547_07	euxassay_003547_08	euxassay_003547_09	euxassay_003547_10

euxassay_003547_11	euxassay_003547_12	euxassay_003547_13	euxassay_003547_14	euxassay_003547_15

euxassay_003547_16	euxassay_003547_17	euxassay_003547_18	euxassay_003547_19	euxassay_003547_20

euxassay_003547_21	euxassay_003547_22	euxassay_003547_23	euxassay_003547_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11096_wholemount_strong.wlz

11096_wholemount_moderate.wlz

11096_wholemount_weak.wlz

11096_wholemount_possible.wlz

11096_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11096_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7940
Entity Detected:	Rasgef1b, RasGEF domain family, member 1B ( MGI:2443755)
Sequence:	sense strand is shown >T7940 GGCTGGAGTTTCAGTATAAACAAGGACCCCGACTGGATCGGCGGGTTTTCCCTTTCTTCTGGCGTTCGAC CCTTCCCGGTCCTGAGCTCGCCTTTTGGGGCGCCTCGGTACGGCAGCCAGGAGGCAGAGGCTCCGAGCTG TGTGCGGGAAAGTATGCCTCAGACGCCCCCCTTCTCAGCAATGTTTGACAGCAGTGGCTACAACCGAAAC CTCTACCAGTCCGCAGAGGACAGCTGTGGAGGCTTGTACTACCATGACAACAACCTCCTTTCTGGGTCTC TGGAAGCCCTTATCCAACACTTGGTACCCAATGTGGATTACTATCCTGATAGGACATACATCTTCACCTT CCTGCTTAGTTCTCGGTTATTCATGCATCCGTACGAGCTCATGGCTAAGGTTTGCCACCTGTGTGTTGAG CACCAGCGACTGAGTGAAGGGGACGGCGATAAGAACCAGATGAGAAAAATTGCACCTAAAATCCTTCAGC TCTTGACAGAGTGGACAGAAACATTTCCGTATGACTTCCGGGACGAGAGAATGATGAGGAACCTCAAGGA CCTGGCGCACAGGATGGCCAGTGGCGAGGAGACGTACCGGAAGAACGTCCAGCAGATGATGCAGTGTCTG ATCCGAAAACTCGCCGCCCTCAGCCAGTATGAGGAAGTCCTGGCCAAACTCAGCTCCACAGCCACGGACC GGCTCACGGTTCTGAAGACCAAGCCCCAGTCCATACAGCGGGACATCATGACCGTCTGCAGCGACCCTTA CACATTGGCCCAGCAGCTGACCCACATCGAGCTGGAGAGACTCAATTACATTGGCCCAGAAGAGTTTGTT CAGGCATTTGTGCAGAAGGATCCTTTGGACAACGATAAGAGTTGCTACAGCGAACGGAAGAAAACACGGA ACTTGGAGGCGTACGTAGAATGGTTTAATCGTCTAAGCTACTTGGTTGCTACAGAAATCTGCATGCCTGT GAAGAAGAAGCACCGAGCGAGGATGATCGAGTATTTCATCGATGTGGCTCGAGAGTGTTTCAACATTGGA AACTTCAATTCTTTGATGGCAATCATCTCTGGTATGAATATGAGCCCGGTCTCGAGACTTAAGAAAACCT GGGCCAAAGTGAAGACTGCAAAGTTTGACATTCTTGAGCATCAGATGGATCCATCCAGCAATTTCTACAA CTACAGAACGGCTCTCCGAGGGGCAGCGCAAAGGTCCCTGACTGCTCACAGTAGCAGAGAGAAGATTGTG ATACCATTCTTCAGTCTCTTAATCAAAGACATTTATTTCCTCAACGAGGGCTGTGTCAACCGTCTTCCAA ATGGCCATGTCAACTTTGAGAAATTTTGGGAACTGGCCAAACAAGTGAGTGAATTTATGACATGGAAACA GGTGGAGTGTCCGTTCGAGCGGGACCGGAAGGTCTTACAATACCTGCTCTCCGTGCCTGTATTCAGTGAA GATGCGCTCTACTTGGCTTCATACGAGAGTGAAGGACCCGAAAATAATATAGAGAAGGACAGATGGAAGT CTTTAAGGTCCAGCCTTTTGGGCAGAGTTTAACATCTGGAAAATGCTGCTGCTGCTGTTGCTGCTGCTGC TGCCACTGCTGCATCAGAGGACCCTGGAGGGGCTGGCCTTTGTTTTCTGGCATCCAGTACTAGGGACTGC CTCCCAGACCCTGCAAGCAAGCCCAGCTGTAGATCAGACGGGAAATTCATTTCCCACAGCTGACAGATGG ACTTGGATCTCTTTTGAGACTGAAATCGTCACTGAAGACACTTGAGGGAGAATCTTCTGAAAGCCCCGGC TCTGCGCATGATTCATCTTCTGGAATTTCCATGTGAATCACAGCTCTGCACCTGGATGGACTTTTGTTTG TGATATATTTTTTTTTTTGGCCTGAACATTTGCTGCTAATGGAACTTACACAGAGTGCGGCTCTATGGAA GCCCAAGTTTCTTTGTTAATTTAAATGAAAAAGGGAGAGGGAGGGAGGGGAAACCCCTCCCACCCACCAA GTTTGGATCCTGGACCTCAGCTTGAACAGTATTCCAGTGAGGGCAAAGATTGGGTGGGGTTCTACTGCAG ACATCCCCTTACCCAGTGCGGTATTGTCAACACCCCTCCCCCGTCCCCCTCTTCCTCCAAGGCTTTGAGT TGGCTGCTGGCTAGCAAACTCCTTCTTACCCATTAAGTTATTTAATATAATAATGAAGCGCAACACTGTG GTAGGAAAATAGCCACTAGGGGGGAAAAAAGGCAGAGATTGTCACTGTCGGACTGCCCATCCTGCAGGAA GGGTCACTGTAGGATTGCTCATCCTGCGGGAAGGGACCATGTTTTCTTTACCCTTCTGAGCTGTTTACAG CTGACCACTGTGTCCTATAGATGTATTTTTCAGTAGGTTTTGTTTTTGTTGTTGTTAACTTAGTTTTCCA TGATGCTGCCCCATCCCCATAAATGTAAATGCTATGACTTTAAGGGTCTTGGGGGGAGGATCCATGACCT AAACACAGTCGTTTTTTTTTTTTCCTAACTTATTTTTCATTATTCTCTGGAGTCAGTTGGTGGCTAAACA TATAAACAAGGAATCCATTAAGAAAACTTACCTCCTCGCCCCGCAGCAGTGACCAAATGCAAACCAGGTT GCTGATAATTCTGATCAACTTTAACATCTAAATTAGGTCTGTAGTTGTTCTGCAGGCAGCCATTACGATA CTCTAGGAGAAACTTTAGAGTAGTGATCCCCTGCTAATGAAAATGCACAATGCCAAGTTCCAGCCATGTT GGTTAACCCTTCTGACGCCATCCTGAGAATCAGCATGTCTTAACCGTGCGTCATCTCAACAGGCTTGGCC TGTCTCCAAGTTGTCCCTTCACAATGTCAATACATTTTAAGCTCTGCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6517502 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6517502 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11097 same embryo
	EMAGE:11095 same embryo
	EMAGE:11098 same embryo
	EMAGE:11094 same embryo
	EurExpress:euxassay_003547 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source