EMAGE mouse gene expression spatial database

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EMAGE:11143

Ppm1g protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform ( MGI:106065)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003451. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003451_01	euxassay_003451_02	euxassay_003451_03	euxassay_003451_04

euxassay_003451_05	euxassay_003451_06	euxassay_003451_07	euxassay_003451_08	euxassay_003451_09

euxassay_003451_10	euxassay_003451_11	euxassay_003451_12	euxassay_003451_13	euxassay_003451_14

euxassay_003451_15	euxassay_003451_16	euxassay_003451_17	euxassay_003451_18	euxassay_003451_19

euxassay_003451_20	euxassay_003451_21	euxassay_003451_22	euxassay_003451_23	euxassay_003451_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11143_wholemount_strong.wlz

11143_wholemount_moderate.wlz

11143_wholemount_weak.wlz

11143_wholemount_possible.wlz

11143_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11143_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6220
Entity Detected:	Ppm1g, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform ( MGI:106065)
Sequence:	sense strand is shown >T6220 CTTGGGTCGACTGCGCAGCCGGGCCCGCTCCTTGCCACGCGCGCCCCCAGGGGCCCCGGCTCCGGCGCCG GCAAGGGTCGCGTCCCGCTTACCTGTTCCTCAGGTAGCCAGCCATCCGCCGCCATGGGTGCCTACCTCTC TCAGCCCAACACGGTGAAGTGCTCCGGGGACGGGGTTGGCGCCCCGCGGCTCCCGCTGCCCTACGGCTTC TCCGCCATGCAAGGCTGGCGCGTCTCCATGGAGGATGCTCACAACTGTATTCCTGAGCTGGACAATGAGA CAGCCATGTTTTCTGTCTACGATGGACATGGAGGGGAAGAGGTTGCCTTGTACTGTGCCAAATATCTTCC TGATATTATCAAAGATCAGAAGGCCTACAAGGAAGGCAAGCTTCAGAAGGCTTTACAAGATGCCTTCTTG GCTATTGATGCCAAGCTGACCACAGAGGAAGTCATTAAGGAACTGGCCCAGATTGCAGGGAGACCCACTG AAGATGAGGATGATAAAGACAAAGTAGCAGATGAGGATGATGTGGACAATGAGGAGGCTGCATTGTTGCA TGAAGAGGCTACCATGACTATTGAAGAGCTGCTGACGCGATATGGGCAGAACTGTCAGAAGGTCCCTCCC CACACCAAATCTGGAATTGGGACAGGCGATGAACCAGGGCCCCAGGGCCTCAATGGGGAGGCTGGACCTG AGGACCCATCTAGGGAAACTCCTTCCCAGGAAAATGGCCCCACAGCCAAAGGCCACACAGGCTTTTCCTC CAACTCGGAACATGGGACTGAGGCAGGCCAAATTAGTGAGCCCGGTACTGCTACCGGTGAGGCTGGACCT TCCTGCTCTTCAGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTTTGAGGACAGTGAAGATG AATCAGATGAGGTGGAGGAAGAGGAGGATGACAGTGAGGAATGTAGTGAGGACGAGGACGGCTACAGCAG TGAGGAGGCAGAGAACGAGGAAGACGAGGATGACACGGAGGAGGCTGAAGAGGATGATGATGAAGAGATG ATGGTCCCTGGAATGGAAGGCAAAGAAGAGCCTGGTTCTGACAGTGGCACAACAGCGGTGGTGGCTCTGA TCAGAGGGAAGCAGTTGATTGTGGCCAATGCAGGAGACTCTCGCTGTGTGGTGTCCGAGGCTGGCAAAGC TTTAGATATGTCCTATGACCACAAACCAGAGGATGAAGTGGAGCTGGCACGCATCAAGAATGCTGGTGGC AAGGTCACCATGGATGGACGAGTCAATGGAGGCCTCAACCTCTCCAGGGCCATTGGAGACCACTTCTACA AGAGAAACAAAAACTTGCCACCCCAGGAACAGATGATTTCTGCCCTTCCTGACATCAAGGTGCTGACTCT CACTGATGACCATGAATTCATGGTCATTGCTTGTGACGGCATCTGGAATGTGATGAGCAGCCAGGAGGTT GTAGACTTTATTCAATCAAAGATCAGTCAACGTGATGAAAACGGGGAGCTTCGGTTATTGTCATCCATTG TGGAAGAGCTGCTGGATCAGTGCCTGGCGCCAGACACTTCTGGGGATGGTACAGGGTGTGACAACATGAC GTGCATCATCATTTGCTTCAAGCCCCGAAACACAGTAGAGCTTCAGGCAGAGAGTGGCAAGAGGAAACTG GAGGAGGCACTGTCCACGGAGGGGGCTGAAGACACCGGCAACAGTGACAAAAAGAAGGCCAAGAGGGACT AGTGGTCAACCGGACCCTGCCCATGTGGACTGTTTTCTGAGCCCTTGGACCCGAGACTGAGTTTTGTCCT TGTCCTTTAGCCTTAGCAGTGGGTATGAGGTGTGCAGGGGGCTGGGTGGCTTTCCTCAGCCCATTACAAA GAGGGCCCCCCACCCCCCCCACGCGGCAGCCTGGGAGGCTCTGCTGTCCTCTTAAGCCTCCTTACTCTCC TTGGGCTCATCGACTATCGGTTCTGTGCCTGTGCTCTGTTGTGTTGGAGGGAAGGACTGGTAGTTCTGAT TTTTACTCTGTGAACACTTTATTTAAGGACATTCTTTTTTATTGGCGGCTCTGTGACCCCTAGCCGCTTG CACCCGCTCTCTGTTGTACACTTTCAAGCAACACTTTTTCAGACTAAAGGCCAAACAAAAGCTAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499081 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499081 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11142 same embryo
	EMAGE:11144 same embryo
	EMAGE:11145 same embryo
	EMAGE:11141 same embryo
	EMAGE:11140 same embryo
	EurExpress:euxassay_003451 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source