EMAGE mouse gene expression spatial database

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EMAGE:11144

Arhgef7 Rho guanine nucleotide exchange factor (GEF7) ( MGI:1860493)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003435. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003435_01	euxassay_003435_02	euxassay_003435_03	euxassay_003435_04

euxassay_003435_05	euxassay_003435_06	euxassay_003435_07	euxassay_003435_08	euxassay_003435_09

euxassay_003435_10	euxassay_003435_11	euxassay_003435_12	euxassay_003435_13	euxassay_003435_14

euxassay_003435_15	euxassay_003435_16	euxassay_003435_17	euxassay_003435_18	euxassay_003435_19

euxassay_003435_20	euxassay_003435_21	euxassay_003435_22	euxassay_003435_23	euxassay_003435_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11144_wholemount_strong.wlz

11144_wholemount_moderate.wlz

11144_wholemount_weak.wlz

11144_wholemount_possible.wlz

11144_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11144_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6226
Entity Detected:	Arhgef7, Rho guanine nucleotide exchange factor (GEF7) ( MGI:1860493)
Sequence:	sense strand is shown >T6226 CGGACGCGTGGGCGGAGATTGGTGCAGCCCCTAGGAAGGGGAGCGGATGAAGCGTCCAGCCCGCTCAGCG CCGCTGTGTGTTGCAGGTCTACCCGGAGCCGCGGAACGAGAGCGAGTGCCTGAGCAACATCCGCGAGTTC CTGCGCGCCTGCGGGGCGTCCCTGCGCCTAGAGACTTTTGATGCAAATGATTTGTATCAGGGGCAGAACT TTAACAAGGTCCTCAGCTCCTTGGTGACACTAAATAAAGTGACAGCAGACATTGGACTAGGAAGTGATTC TGTGTGTGCTCGGCCTTCGTCCCATCGGATAAAGTCTTTTGATTCCCTGGGATCCCAGTCTTCACACAGT AGGACTTCAAAATTGCTTCAGAGCCAGTACCGAAGCTTGGACATGACTGATAACACCAACAGCCAACTGG TAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATGAACTCTCCTTCTCAAAGGGTGATGTCAT CCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCACACACAATGGCAGGACCGGCTGGTTCCCC AGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTGTCACCCAAATCAGGGACCTTGAAGAGCC CTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATTACAACGTGGTGCTACAGAACATCCTGGA AACAGAGCATGAGTATTCCAAGGAGCTGCAGTCTGTGCTGTCCACCTACCTGCGGCCACTGCAGACCAGT GACAAGTTGAGTTCAGCAAACACTTCATATTTAATGGGAAATCTAGAGGAAATATCTTCCTTCCAGCAAG TGCTTGTACAGTCCCTAGAAGAATGCACGAAGTCTCCTGAAGCCCAACAGAGAGTTGGTGGCTGCTTCCT GAGCCTGATGCCGCAGATGAGGACCCTGTACCTCGCTTACTGTGCCAACCACCCATCTGCTGTGAGCGTC CTCACAGAGCACAGTGAGGACCTAGGAGAGTTCATGGAAACAAAAGGTGCCAGCAGCCCTGGGATCCTGG TGCTGACCACCGGCCTGAGCAAGCCCTTCATGCGCCTGGACAAGTACCCCACACTGCTAAAGGAGCTGGA GAGACACATGGAGGATTATCATCCTGATAGACAAGATATTCAGAAGTCTATGACGGCCTTCAAAAACCTT TCAGCTCAGTGTCAAGAAGTTCGCAAGAGGAAGGAGCTGGAGCTGCAGATCCTGACGGAGCCCATCAGGA GCTGGGAGGGGGATGACATAAAGACCCTGGGCAGTGTCACATACATGTCCCAAGTCACCATTCAGTGTGC GGGAAGCGAGGAGAAGAATGAGAGATACCTCCTGCTCTTCCCAAACCTTCTGCTCATGTTGTCTGCAAGT CCCAGGATGAGTGGTTTCATCTATCAGGGGAAGCTGCCAACAACAGGAATGACAATCACAAAGCTTGAGG ACAGTGAAAACCATAGGAATGCATTTGAGATATCAGGGAGCATGATCGAGCGGATTCTGGTGTCCTGCAC CAGCCAGCAGGACTTACACGAGTGGGTGGAACACCTGCAGAAGCAGACGAAGGTCACATCTGTGAGCAAC CCCACCATCAAACCCCACTCGGTGCCATCACACACACTTCCTTCCCATCCTCTCACTCCATCCAGCAAAC ACGCGGACAGCAAGCCCGTGGCACTGACGCCTGCGTACCACACACTCCCCCACCCCTCTCACCATGGCAC CCCACACACCACCATCAGCTGGGGACCCCTGGAGCCTCCGAAGACCCCCAAGCCTTGGAGCCTGAGTTGC CTGCGGCCTGCACCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTCAGTAAGAGCCCCA AGACCATGAAAAAGCTGCTGCCGAAGCGCAAGCCCGAGCGGAAGCCTTCGGACGAGGAGTTCGCTGTGCG CAAGAGCACAGCGGCGCTGGAAGAAGACGCTCAGATCCTGAAGGTTATCGAAGCTTATTGCACAAGTGCA AAGACGCGCCAGACCCTGAACTCAAGTTCACGCAAAGAATCTGCTCCACAAGTGTTGCTTCCAGAAGAAG AAAAAATTATAGTCGAAGAAACCAAGAGCAATGGGCAGACAGTGATAGAGGAAAAGAGCCTCGTGGATAC GGTGTATGCGTTAAAGGATGAAGTCCAGGAGTTAAGACAGGATAACAAGAAGATGAAGAAGTCCTTAGAG GAGGAACAGAGGGCCCGCAAGGACTTAGAGAAGCTGGTGAGGAAGGTTCTAAAGAATATGAACGACCCTG CCTGGGATGAGACCAATCTATAGGCTGACTTCATTCTCATAGGCTGAAGAGCAGCAGTTCCTGGACATGG CTGGGGCCCTTGGCTTCCAGCACAGTGACATTTAGGACTAAGGAAGGTGGTCTCCATCAGGCGAGCAGCA GCAATGAGGCTGTTGCTGAAGTAGCTACATGTGAGTTATAGACACTGAGGTCTGGGCTGAGTGTGCACAT ATCTGCCACTTCTCCATGCAGTGACTGACCAGAGTCCTTTGGCTTCTGGACTTGAGTTGAATCTATAAAT AGCATACCTGTGTACTCCTTGTAAATACTGGGATCTGAGCACCTACCTGAGATGGGACTGGGCTAACCCT CCTGCTAGGATTTCCTTCTCAGCCTTTCTGGCCACAGGGATCTGAAGTTGTGTTTGTGAAGTGTTGGAAG CAGAAAGGTGAAGAGATGCCGTGGCAGCTGTCTGACTCGTGTGTAGCGCAGGCTCCATGGTGCCATCGGG AACAGGCACTCGGGCACAGTGAATTTGTGTGTCTCTGTTCTTAACTGCAGTATTCCAGTCTCGGCTATGG CTGTGCCCAGGGCATCATGGGTTCTAGGGAGGGAAACTGTTACCTGCCTGTGGTGCCCGCCTCTGCTGCC CTGCCCTGTCCTGTCTGGGCCTTGGTCACAGTGTGCAGAAGTGGGTTAGCCCTGTACATAGATGTGTTAT TTATTCCATACTCTCTAGCCGGTGCTGCCGGCCGGGCAGGGTTTGGCTGCAGGCTGTTTTCATCCAGGAG CTCTGCTCTGCCTCTGGCCGCAGTCAGCGTTGTCCAGGCCACTGAAAGCCCTCCTAACCCTCTGAGCGAG AATAAAGCAATACAAACCCCAAGTGGAGCAGCACAGCGAGAGCCCTCAAGTGCTGTGCCTGCGCCCTACT CTGCTGCCTCCCCAGGCCAGGCCCAGGCAGGCCCTGGAAGGCTGGTGGCCTGGGCAAGCGGCCCTGCTTC TGTTAGGCCATGCTTGCCCCTGGTTTGGCAACAGTTAACAGTGACCTCAGGAGCTTAGCTGTCCCTTCTT TCCTCCAAAGAGGTTGTTTTCAAGTCAGAGATACTTTCCCTTAGAAAGGTGTTTTGTCTTTAAACACTGA AGAGCAATGTGTGCTACTGGGGCCTCAGCACAGCCTCTTTCCCTAGGACGTGACCCAGCCCCCGCCCACC CACCCACCTGTGGAGGTTTCTGCGTGGGCACTTAGAGGCCGTTTGTGCTCTCTCCTTCTGCCACACTCTA GCTGGTTGCAGTGTCTATGGGTTGTGTCTGTCAGGATCCACCAGAGCCTCCCACCCCTGTTAGCATTTAC AGCTCAGTGTGCGGGGTGAGAAAAGCTGAGCTGTTCCTTTCCTTTTGGTAAGCCCTGTTTTCCAAGCACT CTGCTGACCCAGAAGACACTGCAGCAAAAGGCAGTGTGTTTGCTGTCACAGCAGGTCTCAACTCAGGAAC AACACTCCTTGCAACTCCACTGCTGGCACAGGGGCCTGGGGTTGCTGGGCAGCCCCTCCATCAACAGGAA GCAAAGACCATGTTCGCCAGTGTCCTTGCCACCGCCGAGGTTGCCGTCCGTGCCCCAGGACAATTCCTTT CAGACCTGCCTTAATGATTCGCTTGGAGTGTTCCATGAGTATTCATGTTCCCAGCATCCCTACCCTGGAC AGGGTGGTTAGGCTGTCAGCACGCCGCCTTGTCTCTCTGAGGATCCAGTGTCTTCCAGGCTCTCCAGGGA GCGAGAATCTGCACCCAAGAGCAGTTCTTTTTAAAACCGGCCCTGGTGTACACACCCACTTCTCTCTCCT GCGTGTCTGCGCGGGGAGCTTTGATACCAACAGCACAGGGCGCCTCTCACCAACTCCTAAGTCCTCTGTG TTCTGTAAGGGATGCGGGGGAATCAAGCCATTTCCTGTCGTGTAGATGTGAAGCACTTTCCGTTCTGAAC CATGCTGCGGTGTAGCAGCGTCCGAAACTCCACTGTTCTCCTCCAGCTGCTGCCCATGCGCCCTGTGCAC TTCATGCCAACCCCCACTTTCCTGGGACACGACAGGAGACCGCACCGGGGTCGACTGCTCAGTGTGTGTG TGCTACACCCTTGCAATTATTAAACTCCTTATTTTTCTTATCTCACAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499604 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499604 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11142 same embryo
	EMAGE:11143 same embryo
	EMAGE:11145 same embryo
	EMAGE:11141 same embryo
	EMAGE:11140 same embryo
	EurExpress:euxassay_003435 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source