EMAGE mouse gene expression spatial database

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EMAGE:11531

Ctla2a cytotoxic T lymphocyte-associated protein 2 alpha ( MGI:88554)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006744. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006744_01	euxassay_006744_02	euxassay_006744_03	euxassay_006744_04

euxassay_006744_05	euxassay_006744_06	euxassay_006744_07	euxassay_006744_08	euxassay_006744_09

euxassay_006744_10	euxassay_006744_11	euxassay_006744_12	euxassay_006744_13	euxassay_006744_14

euxassay_006744_15	euxassay_006744_16	euxassay_006744_17	euxassay_006744_18	euxassay_006744_19

euxassay_006744_20	euxassay_006744_21	euxassay_006744_22	euxassay_006744_23	euxassay_006744_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11531_wholemount_strong.wlz

11531_wholemount_moderate.wlz

11531_wholemount_weak.wlz

11531_wholemount_possible.wlz

11531_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11531_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

heart ventricle

strong

spotted

strong expression: see section 07 08 09 10 11 14 15 16 19 moderate expression: see section 12 13 17 18

esophagus

strong

regional

strong expression: see section 14

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

moderate

regional

moderate expression: see section 16

bladder

moderate

regional

moderate expression: see section 16

left lung

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 moderate expression: see section 04

right lung

strong

regional

strong expression: see section 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8220
Entity Detected:	Ctla2a, cytotoxic T lymphocyte-associated protein 2 alpha ( MGI:88554)
Sequence:	sense strand is shown >T8220 CAGAGACCGTGGACAACAAAATGATGGTTTCTATCTGTGAACAGAAGCTGCAGCACTTCAGTGCTGTCTT CCTGCTCATCCTCTGCTTGGGAATGATGTCAGCTGCTCCACCCCCTGATCCAAGTTTGGATAATGAGTGG AAAGAATGGAAGACGAAATTTGCAAAAGCCTACAATCTGAATGAAGAAAGACACAGAAGACTCGTGTGGG AGGAGAATAAGAAGAAAATTGAGGCACACAATGCAGACTATGAGCAGGGCAAGACCAGCTTCTACATGGG CCTGAATCAATTTAGTGACTTGACTCCAGAAGAATTCAAGACAAATTGCTATGGAAACTCACTGAATAGA GGAGAAATGGCTCCTGATTTGCCTGAATATGAAGATTTGGGAAAGAACAGCTATCTGACACCTGGAAGGG CTCAGCCAGAGTAACAGCTGTGGCTTGACTGGTAACAATAGCATAGGCTAGAAGTGAAGCAAGCACTCTG CCATTGCCTTGGAAGTCTTCTGTCTCCTGGTATGAGAGGAATGATGCTGGAGAGGCTCAACAGCAGGAGG CATCCCATCACAGCTTCCAGCACCTGCTTTGTGTAGTGTAACATTGAATTCATGTAAGATTAACACTGTG GTGTAAACTCTGAGGCACATTCATGCAATAAAACTACTAGTTTTGGCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1244311 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1244311 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11534 same embryo
	EMAGE:11530 same embryo
	EMAGE:11533 same embryo
	EMAGE:11529 same embryo
	EMAGE:11532 same embryo
	EurExpress:euxassay_006744 same experiment
	MGI:4824105 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source