EMAGE mouse gene expression spatial database

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EMAGE:11532

Clcnka chloride channel Ka ( MGI:1329026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006769. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006769_01	euxassay_006769_02	euxassay_006769_03	euxassay_006769_04

euxassay_006769_05	euxassay_006769_06	euxassay_006769_07	euxassay_006769_08	euxassay_006769_09

euxassay_006769_10	euxassay_006769_11	euxassay_006769_12	euxassay_006769_13	euxassay_006769_14

euxassay_006769_15	euxassay_006769_16	euxassay_006769_17	euxassay_006769_18	euxassay_006769_19

euxassay_006769_20	euxassay_006769_21	euxassay_006769_22	euxassay_006769_23	euxassay_006769_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11532_wholemount_strong.wlz

11532_wholemount_moderate.wlz

11532_wholemount_weak.wlz

11532_wholemount_possible.wlz

11532_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11532_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

inner ear

strong

regional

strong expression: see section 06 23 24 moderate expression: see section 04 05 07 08 09 10 11 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4760
Entity Detected:	Clcnka, chloride channel Ka ( MGI:1329026)
Sequence:	sense strand is shown >T4760 CCACGCGTCCGAGAAGCACCAGCCAGGCAGGACCTCTACAGGGTGGACCGGGAGGACGGATGGAAGAGCT GGTGGGGCTGCGTGAGGGCTCCTCCGGGAAGCCGGTGACTCTGCAAGAGCTATGGGGTCCGTGTCCACGC ATCCGCCGAGGCATCAGAGGGGGCCTAGAGTGGCTGAAAGAGCGGCTGTTCCGTGCGCGTGAGGACTGGT ACTTCCTGGTGGCTCTTGGGGTGCTCATGGCTCTGATCAGCTATGCCATGAACTTTGCTATCGGGCGTGT GGTCAGAGCACACAAGTGGCTGTACAGAGAAGTTGGGGACGGCCACCTGCTCCGGTACCTCTCCTGGACC GTGTACCCCGTAGCTCTTCTGTCCTTCTCCTCCGGCTTCTCACAGAGCATTACGCCCTTCTCTGGAGGGT CTGGACTCCCAGAGCTGAAGACCATGCTGTCTGGAGTAGTCCTGGAGAACTACCTAGACATCAAGAACTT CGGGGCCAAGGTGGTGGGCCTCTCCTGCACCCTGGCCACAGGCAGCACCATCTTCCTAGGCAAAGTGGGC CCGTTCGTGCACCTGAGTGTGATGATTTCTGCTTATCTGGGCCGCGTGCGCGCCAAAGCCGTCGGGGATA CTGAGAGCAAGGCCAAGGAAGTTGAAATGCTGTCGGCTGCAGCGGCGGTGGGCGTGGCCACAGTCTTCGC TGCCCCCTTCAGTGGTGTCCTGTTCAGCATTGAGGTCATGTCCTCTCACTTCTCCGTCTGGAATTACTGG AGGGGCTTCTTCGCTGCCACCTGCGGGGCCTTCATGTTCCGTCTCCTTGCAGTCTTCAACAGTGAACAGG AGACCATCACCTCCATCTACAAGACCAGATTCCGAGTGGATGTGCCCTTTGACCTGCCTGAAATCTTCTT CTTTGTGGCTCTGGGGTTCATCTGCGGGATCCTCAGTTGCGTTTACCTGTTCTGTCAGCGAAATTTTCTC CGTTTCATCAAGACCAATCGCTACACCTCCAAACTGCTGGCTACAAGCAAGCCGTCCTATGCAGCTCTGG TCGCGCTGGTCCTGGCCTCAGTCACCTATCCCCCTGGTGTGGGCCGTTTCATGGCTTCCCGGCTGTCCAT GGCGGAACACTTGCACTCTCTGTTTGACAACAACTCATGGGCATTGATGACTTGGAATGCGTCCCCACCC TGGCCTGCTGAGCCTGACCCCCAGAACCTGTGGTTTGAATGGTACCACCCGCGGTTCACCATCTTTGGGA CGCTGGCCTTCTTCCTGATCATGAAGTTCTGGATGCTGATTCTGGCCACCACGATCCCCATGCCTGCTGG CTACTTCATGCCTATATTCATCATCGGAGCTGCCATCGGGCGCCTCTTGGGAGAGGCCCTGTCTGTCGCT TTCCCAGAGGGCATTGTGGCTGGCGGAGAGGTCTATCCCATCATGCCTGGGGGCTATGCTCTGGCAGGTG CTGCTGCCTTCTCCGGAGCAGTGACCCACACCATCTCCACAGCGCTGCTGGCCTTTGAGCTGACTGGCCA GATCGTTCACGCACTGCCTGTGCTGATGGCTGTGCTGGCGGCCAATGTCATCTCCCAAAACTTACAGCCC TCTTTCTACGATGGCACCATCATGGCCAAGAAACTGCCGTACCTGCCGTGGATCCGTGGCCGACAGATTG GCTCCTACCCTGTAACGGTGGAACATTTCATGAATTCTAACCTCACCACACTGGCCAAGGACATGCCCCT GGAGGAGGTGGTCAAGGTGGTGACCTCCACAGATGTGTCTCAGTATCCCTTGGTGGAGACCAGAGAGTCT CAGACCCTGGTGGGCATTGTGGAAAGGACCCACTTGGTGCAAGCCCTTCAGACCCAGCCAGCTTCCTGGG CTCCAGGCCAAGAGCGATTTCTCCAGGACATCTTGGCTGGTGGCTGCCCCACACAGCCAGTGACCCTGCA GCTGTCCCCAGAGACCTCCCTATATCAGGCACACAGGCTCTTTGAGCTGCTGACCCTTCAGGCGCTGTTC GTGACGTCACGGGGCAGAGCTGTGGGCTCTGTGTCCTGGGTGGAGCTGAAGAAAGCCATTTCCACCTTGA TCAACCCACCAGCCCCCAAGTGAGTCGTGTGGGGGTCGGAACAGGAACCCAGCTGAGGGCTCCAGATGCT GCACAGAGCCCTGCCCCTTTTCCCTCCCTCCCTGCACTCCTCCAGCCCAGCCCAGCTACTCAGTGCATCA GCCAGCTCTACCCAGCTGAACAGGGCTCCACATTTATCACGGCCCCCCGAGAGGACAACATCTCTCTGGT CTGGAAGGGACAAATCTCACCCCGGTGGACGGGACGTACCTTCTGGGATTATCTATTCACAACCATTAGC TCCTGAGAAAATAAAACTAAAGCTGATTCCCAGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5042736 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5042736 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11534 same embryo
	EMAGE:11531 same embryo
	EMAGE:11530 same embryo
	EMAGE:11533 same embryo
	EMAGE:11529 same embryo
	EurExpress:euxassay_006769 same experiment
	MGI:4823903 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source