EMAGE mouse gene expression spatial database

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EMAGE:11777

Ugp2 UDP-glucose pyrophosphorylase 2 ( MGI:2183447)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006932. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006932_01	euxassay_006932_02	euxassay_006932_03	euxassay_006932_04

euxassay_006932_05	euxassay_006932_06	euxassay_006932_07	euxassay_006932_08	euxassay_006932_09

euxassay_006932_10	euxassay_006932_11	euxassay_006932_12	euxassay_006932_13	euxassay_006932_14

euxassay_006932_15	euxassay_006932_16	euxassay_006932_17	euxassay_006932_18	euxassay_006932_19

euxassay_006932_20	euxassay_006932_21	euxassay_006932_22	euxassay_006932_23	euxassay_006932_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11777_wholemount_strong.wlz

11777_wholemount_moderate.wlz

11777_wholemount_weak.wlz

11777_wholemount_possible.wlz

11777_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11777_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08

kidney calyx

strong

regional

strong expression: see section 07 08 09 10 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9924
Entity Detected:	Ugp2, UDP-glucose pyrophosphorylase 2 ( MGI:2183447)
Sequence:	sense strand is shown >T9924 TGGGATGAAATTTAAGTTCGTCTGGTTTTACCTTTTCCGGAGTCTCCAGTCGTCCCCTGTTGGTGTCGGG ATCGCTGAGTAGCCTGGAGTTCCCTCCCAAGCTGAGTCGGTCCCACCAAGTTTTCCGTCTTCAGGATTTT TCTTTTTAAAGACTAGTTTTGATTTTTTTTCCCCCCGAATTCTTCTTTAAGTAGTTAAACATCGGAGGAG AAAGAACACTTCGGTTGCAGGGAAGGGAGAGAGACCTTCCTTACAACGTAGATTCTAGAAATTGAAAATT ATAAGCCAGATTATTTTAATAAGATCCTAAAATGTCGAGATTTGTACAAGATCTTAGCAAAGCTATGTCT CAAGATGGTGCTTCTCAGTTCCAAGAGGTCATTCTCCAAGAACTAGAATTATCTGTGAAGAAAGAATTAG AAAAAATACTTACCACAGCAGCCTCACATGAGTTTGAGCACACTAAAAAAGATCTTGATGGATTTCGGAA GCTATTTCATAGATTTTTGCAAGAAAAGGGACCGTCTGTGGACTGGGGTAAAATCCAGAGACCTCCAGAA GATTCGATTCAACCCTATGAAAAGATAAAAGCCAGAGGCTTGCCTGATAACATATCTTCTGTGTTGAACA AGTTGGTGGTAGTGAAACTCAATGGTGGTTTGGGAACCAGCATGGGCTGCAAAGGCCCTAAAAGTCTGAT TGGTGTAAGAAATGAGAACACCTTTTTGGATCTAACTGTTCAGCAAATTGAACATTTGAACAAAACCTAT AATACAGATGTTCCTCTCGTTTTAATGAATTCTTTTAACACGGATGAAGATACCAAAAAAATACTCCAGA AGTACAATCACTGTCGTGTGAAAATCTACACCTTCAATCAAAGCAGGTACCCGAGGATCAATAAGGAATC TCTACTTCCTATAGCAAAGGATGTGTCTTATTCAGGGGAAAATACTGAAGCTTGGTACCCTCCAGGGCAT GGAGATATCTATGCTAGTTTCTACAATTCTGGCTTGCTCGATACCTTTATAGAAGAAGGCAAAGAGTATA TTTTTGTGTCTAACATAGATAATCTGGGTGCCACAGTGGATCTTTATATTCTTAATCACCTGATGAACCC ACCCAATGGGAAGCGCTGTGAGTTTGTCATGGAAGTCACAAACAAAACACGAGCAGATGTTAAGGGTGGG ACACTCACTCAGTATGAAGGCAAACTGAGACTGGTGGAAATCGCTCAAGTGCCCAAAGCACACGTTGATG AGTTCAAGTCTGTGTCAAAATTTAAAATATTTAACACAAACAACCTATGGATTTCTCTTGGAGCAGTTAA AAGACTTCAGGAACAGAATGCCATTGACATGGAAATCATTGTGAATCCAAAGACATTGGATGGAGGCCTG AATGTAATTCAGTTAGAAACTGCAGTTGGAGCTGCAATTAAAAGTTTCGAGAATTCTTTAGGTATTAATG TTCCAAGGAGTCGTTTTCTGCCTGTGAAAACCACATCAGATCTCTTGCTTGTGATGTCAAACCTCTATAG CCTTAACGCAGGATCTCTGACCATGAGTGAAAAGCGTGAATTCCCTACAGTGCCCTTGGTTAAATTAGGC AGTTCTTTTACCAAGGTTCAAGATTATCTAAGAAGGTTTGAAAGTATACCCGATATGCTGGAACTGGACC ACCTCACTGTTTCAGGAGATGTGACATTTGGAAAAAATGTTTCACTAAAGGGAACAGTTATTATTATTGC AAATCATGGTGACAGAATTGACATCCCCCCAGGAGCCGTGTTAGAGAACAAGATTGTATCTGGAAACCTT CGGATCCTGGATCACTGAAATAAGCACTTTCAGATACACTTTCTACTAATTATGGGCTAAAGAGTTTCTT ATAATAAAATATCCTCTAAGATTATAAAATAAGCAGGTACTTTATTTACTATGTTGTACTCTGCAGTGTT GATTTTTTAAAGTAGTTTTCTGCAATGAGCTTTTAGTCTAAGGAAAGCATAGATGAAGCAATACTTTCCT TCTTTGATGAAAAGCCTAGAAATTAGTTCCTCTTAAAGTGCAATATTACTTAACCTCAGAATCGGGCCAG TTCTGTAGATCCTCAAGAGAAGCTTCAGTGACTGTCCCTTTGAATTGCCTCCGATTTCAAAAATAAATAA ATAAATCTACACGTTTACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30250244 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30250244 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11779 same embryo
	EMAGE:11780 same embryo
	EMAGE:11778 same embryo
	EMAGE:11775 same embryo
	EMAGE:11776 same embryo
	EurExpress:euxassay_006932 same experiment
	MGI:4829078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source