EMAGE mouse gene expression spatial database

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EMAGE:12109

Synrg synergin, gamma ( MGI:1354742)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008403_01	euxassay_008403_02	euxassay_008403_03	euxassay_008403_04	euxassay_008403_05

euxassay_008403_06	euxassay_008403_07	euxassay_008403_08	euxassay_008403_09	euxassay_008403_10

euxassay_008403_11	euxassay_008403_12	euxassay_008403_13	euxassay_008403_14	euxassay_008403_15

euxassay_008403_16	euxassay_008403_17	euxassay_008403_18	euxassay_008403_19	euxassay_008403_20

euxassay_008403_21	euxassay_008403_22	euxassay_008403_23	euxassay_008403_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12109_wholemount_strong.wlz

12109_wholemount_moderate.wlz

12109_wholemount_weak.wlz

12109_wholemount_possible.wlz

12109_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12109_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9213
Entity Detected:	Synrg, synergin, gamma ( MGI:1354742)
Sequence:	sense strand is shown >T9213 GCAAGATGGCGCTGCGGCCGGGAGCCGGAGCCAGCGGCGCGGCGGGGGCGGGCGCGGGGCCCGGCGGGGC AGGCAGCTTCATGTTTCCTGTTGCAGGCGGAATGAGACCCCCTCAAGGCCTGATTCCAATGCAACAGCAA GGATTCCCTATGGTCTCTGTCATGCAGCCCAATATGCAAGGCATGATGGGAATGAATTACAGCTCTCAGA TGTCCCAAGGACCGATTGCAATGCAGGCAGGAATACCAATGGGGCCAATGCCAGCAGCGGGTGTGCCTTT CCTGGGTCAGCCACCTTTCTTGAGCATGCGTCCTGCAGGCCCACAGTACACTCCAGACATGCAGAAGCAG TTTGCGGAAGAGCAACAAAAACGATTTGAACAACAACAAAAGCTCTTAGAAGAAGAAAGAAAAAGACGTC AGTTTGAAGAGCAAAAGCAAAAGCTCAGACTTCTCAGCAGCGTGAAGCCCAAGACAGGAGAGAAGAACAG AGACGATGCTTTGGAAGCCATAAAAGGAAACTTGGATGGGTTTTCCAGAGATGCTAAGATGCACCCCACT CCAGCATCACATCCAAAGAAACAAGGTGTGGGCGTGTTTCCCTCTCAGGACCCTGCTCAGTCCAGAATGC CTCCTTGGATTTACAATGAGAGTTTGGTTCCAGATGCCTATAAGAAAATTTTAGAAACCACAATGACTCC AACTGGAATAGATACTGCTAAACTTTATCCCATTCTGATGTCATCTGGACTTCCCAGGGAAACTCTCGGA CAGATATGGGCCTTAGCTAATCGAACCACACCTGGCAGGCTCACTAAGGAGGAGCTTTACACGGTTCTCG CCATGGTAGCAGTAACACAGAGGGGTGTTCCTGCCATGAGCCCTGATGCTTTGAGCCAGTTCCCTGCGGC TCCCATCCCTACCTTAAGCGGCTTTCCTATGACTCTGCCTACCCCAGTGAGCCAGCCAACGGCGATGCCC TCTGGCCCTACAGGCTCCATGCCCCTCACCCTTGGACAACCCATCATGGGTATCAACCTTGTTGGACCAG TGGGTGGAGCTGCAGCACCAACTTCCAGTGGCTTCATGCCAGCCTACCCATCAAACCAGGTGGGAAAGAC AGAAGAAGATGACTTCCAGGATTTTCAAGATGCTTCGAAATCAGGATCCATCGATGATTCCTTCACTGAC TTCCAAGAGATGCCTGCTTCCTCAAAAACCAGTAATTCCCAGCATGGAAACAGTGCTCCATCACTCTTGA TCCCATTTCCTGGAACTAAAGCCTCCACAGACAAATACGCAGTGTTCAAAGGGATTTCCACTGACAAGCC CTCTGAAAACCCTGCTTCGTTTGGAGAGTCTGGTGATAAATACAGTGCTTTCAGGGAACTCGAGCAAACG ACAGACAGTAAACCTTTAGGAGAGAGCTTTGCCGAGTTCAGATCCACAGGAACCGATGATGGCTTCACTG ACTTCAAGACCGCCGATAGTGTGTCACCCCTAGAGCCACCCACAAAAGACACTTTCCCATCAGCTTTTGC CTCAGGAGCTGCACAGCAGACACAGACACAGGTGAAAACCCCTCTGAACTTAGAAGATCTAGATATGTTC TCCTCAGTGGATTGCAGTGGCGAGAAACAGGTGCCCTTTTCAGCTACATTTAGCACAGCAAAGTCCGTTT CCACTCGACCTCAGCCAGCGGGCTCTGCAGCAGCGTCAGCAGCACTGGCATCCACTAAAACTTCTAGTTT GGCAGATGATTTTGGAGAGTTCAACCTCTTTGGGGAGTATTCAAACCCTGCATCTGCTGGGGAGCAGGAC GACTTTGCAGATTTTATGGCTTTCGGTAACAGCTCCATTTCATCTGAGCCAAAAGCAAGTGACAAGTATG AAGCCCTCAGGGAGGAAGTGAGTCCCAGCCCCTTGTCCAGCAGCACTGTGGAAGGGGCACAGCACCCACC TGCTGCGGCAACCAAGTATGATGTCTTCAAACAGCTGTCCCTAGAGGGCGCTGGGCTGGCCATGGAGGAG TTCAAAGAGAACACTTCCTCCACCAAGAGTGAAGATGACTTTGCCGACTTCCACTCCAGTAAATTCTCAT CCACGAGCTCAGACAAATCCCTGGGAGAGAAAGCCGTGGCTTTCAGACATGCCAAAGAAGACTCCTCCTC GGTGAAGTCTTTAGACCTCCCCTCCATCGGTGGCAGCAGTGTTGGCAAGGAGGACTCTGAAGATGCACTT TCTGTTCAATTTGACATGAAATTGGCTGATGTGGGAGGAGATCTTAAGCATGTCATGTCTGATAGCTCTT TGGATTTACCAACAGTTAGTGGCCAGCATCCTCCTGCCGCAGGATCGGCCTTGGCCAGTGAGGACGCTCT TCCAGAGACTGCCTTCCCAGCTTTTGCCAGCTTTAAAGACATGATGCCTCAGACCACTGAGCAGAAAGAG TTTGAAAGTGGGGACTTCCAGGATTTCACCAGGCAGGACATGCCCACGGTGGACCGCAGCCAAGAAACCT CATGTCCAAGCCCGGCTTCCAGTGTGGCTTCGCACGAGACCCCCAAGGAGGGTGCAGACGACTTTGGAGA ATTTCAAAGTGAAAAGTCCAAAATCAGCAAGTTTGACTTCTTAGTAGCTAATTCCCAGAGCAAAATGAAA TCCAGTGAAGAAATGATCAAAAGTGAGCTGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGA GCTTGAGCCTTGGGGATAAAGAAATAAGCCGGTCCTCTCCTTCCCCGGCCCTGGAGCAGCCCTTCAGAGA CCGGTCCAACACTCTGAGTGAGAGGGCTGCGCTGCCCGTCATCCGAGACAAGTACAAGGATCTCACTGGC GAGGTGGAGGAAAATGAGAGATACGCGTATGAGTGGCAGAGATGCCTAGGGAGCGCGCTGGATGTCATTA AGAAGGCAAATGACACCTTAAATGGCATTAGCAGCAGTGCTGTTTGCACAGAAGTTATCCAGTCGGCTCA AGGCATGGAGTATCTTCTAGGTGTGGTGGAAGTGTACCGCGTAACCAAGCGTGTGGAGCTGGGGATAAAA GCCACTGCTGTGTGTAGTGAGAAACTCCAGCAGTTGCTGAAGGACATCGATAAAGTGTGGAACAACTTAA TCGGCTTCATGTCACTTGCCACACTCACACCAGATGAGAATTCACTGGATTTTTCCTCCTGTATGTTACG GCCCGGGATCAAAAACGCTCAGGAGCTGGCTTGTGGAGTGTGTCTCTTAAATGTGGACTCCAGGAGCCGG GCATTCAACTCGGAGACAGACAGCTTCAAGCTGGCCTACGGAGGACACCAGTACCACGCCAGCTGTGCCA ACTTCTGGATCAACTGTGTTGAACCCAAGCCTCCCGGCCTCCTCTTGCCAGATCTGCTCTGAAAAACTGC TCAATGAAGCTGATTTTTGGTTGTTTCTGTCACAGGACTCAGGGTGACAGAAATCAATAAATAGAATGCA AGTACTGCCAAGCCCCCTGCCACTGGTCTCTTTGAAGCATTCCCCAGGAGGTAGGGTCCCACCAGAAAAC CTGTCACTGCTTCTGCCTGCCCCTCTTTGAGACTTGAGGTAAAATTACCAGACCCCAACTGCACGTGGCA CTGGACATTTGCTTGCTTATCTCCTCTGGTTTTGTTGTTGTCGATGGTGACGTTGGATTGGTTTTCTTTT TTAAGGATAGGGCCTTGATGCATACCCCTGGTTGACCTTCAGTTTGCATCGATCCTCTCAGGTCTGCCTC CCAAGTACTGGAGCACAGGCATGTGCCACCATACCCTATTACTCTTGTCCTCTTTATATAATTTAAAATG CAGACCAGAGTTTTCTGTGAGGAGTAATCTGCTGCTGAAGTGTATGTGAAACGTTAACAAAAGAAGAAAA ATAAGTGATGCTAGGGCTGGAATCCGCAAGTTTGTCTTCAGCCGGCTCGCTTATATCGCCGTCATCATGG CTCTAGGAAGCAGAACACATTTTTTTTTCTGCATTTAGCTTTTAAACACTTGAAGTGAATAAAAAAAAGG CTTGTGATTAAACCGATGATGCTCTGTCCTGGTAAAGAGAGCACTGCTGTCTCCACTGGACAGTGGCAGG CAAGGAGGACACATAGTCACCTCCTGTGGCACCTCACCCTCTTCCCCTTCCTGAAACTCAGCTCTGAAGG AGACCCTCCCTGCCATGACAGGGCCCAGAGCCTGCATCCACGATGCACGCCATTCTCCTTGGAGCCTTTG TTAAGAATCTGCTGCTTTGCCCCGACATCAAGCATGACACAAAGCATGGCGTTGTCCCACCAGGTCACAG CCGTGAACTGGAATCTCTTCGTTGCCACCTCTCTCCACAAGAAGACTGCAGAGAAGGCTGGTGAGATGGC TCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGC TCACAACCATCCGTAACAAGATCTGACTCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACA TATAATAATAAATAAATCTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831893 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831893 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12106 same embryo
	EMAGE:12111 same embryo
	EMAGE:12108 same embryo
	EMAGE:12107 same embryo
	EMAGE:12110 same embryo
	EurExpress:euxassay_008403 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source