EMAGE mouse gene expression spatial database

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EMAGE:14197

S100a14 S100 calcium binding protein A14 ( MGI:1913416)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003998. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003998_01	euxassay_003998_02	euxassay_003998_03	euxassay_003998_04

euxassay_003998_05	euxassay_003998_06	euxassay_003998_07	euxassay_003998_08	euxassay_003998_09

euxassay_003998_10	euxassay_003998_11	euxassay_003998_12	euxassay_003998_13	euxassay_003998_14

euxassay_003998_15	euxassay_003998_16	euxassay_003998_17	euxassay_003998_18	euxassay_003998_19

euxassay_003998_20	euxassay_003998_21	euxassay_003998_22	euxassay_003998_23	euxassay_003998_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14197_wholemount_strong.wlz

14197_wholemount_moderate.wlz

14197_wholemount_weak.wlz

14197_wholemount_possible.wlz

14197_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14197_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

single cell

moderate expression: see section 09 10 12 13

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 09 10 11 12 13 14 15 16 17 18 19 20 24 moderate expression: see section 07 08

vibrissa

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 05

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 13 14 16 17

esophagus

strong

regional

strong expression: see section 10 11

oral epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 06 07

bladder

moderate

regional

Expression in the fundus region.

urethra of male

weak

regional

weak expression: see section 11 12

rectum lumen

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5000
Entity Detected:	S100a14, S100 calcium binding protein A14 ( MGI:1913416)
Sequence:	sense strand is shown >T5000 CCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGCCCAGCTGCCAGCAGGTAGATCATGATCCATCAGC TCCTGTGGGGCAGGCTATAGGACAGACGACAAAACTCAACTCACAGAAGGAAGGACCAGTGTACCAGGAA CGATGGGACAGTGTCGGTCAGCCAATGCTGAGGATGCCCAAGAATTCAGTGATGTAGAGAGGGCCATTGA GACACTCATCAAGAACTTCCATAAATACTCTGTGGCGGGTAAAAAGGAAACACTGACCCCTGCTGAGCTT CGAGACCTGGTTACCCAGCAGCTGCCACACCTCATGCCGAGCAACTGTGGGTTAGAAGAGAAAATTGCCA ACCTGGGCAACTGTAATGACTCGAAACTGGAGTTTGGAAGCTTCTGGGAGTTGATTGGAGAAGCAGCCAA GAGTGTGAAGATGGAGAGGCCTGTTACTCGGAGCTGAGGACTTCTACTTGGAACTTGTTGGGGGTGTTGG GGATAGGGGAGTTTTAGAGGCACTGGAAATAAAACCCTCAATGCCCACCACCCCCTTCCCCAGCCTGCAC CTCTCCTCATTGCTGCAATGTTCACGTTCAGGACAGGCTTCCCTGTGGGCTCCATGGAGCTCCTGGGTCC AGAAGTCCTCATCTCAAGGGAGCTCAGGGGGTGGGTTGGGGCTGGAGAGGATATGCAGGGATCCTGGAAG GGTAAGGGCCAAGCAATTTGGTAGTAGGGGAAGGGCAGAAAGGAACTGGGTTATGGAAGTGATCCAAAGA GCAGGGATGGGAATCTGGCTGCATATTTGGTCCTGAAAAGGGTGTCTGAGAACCTACCCCCTTCTAATCT TGTCCCACCTAAACTGTAGTTGTCTGCCCTGTGCTATCCTTGCTGCTTCCAGCTCTGCCCCATCCTCCTT CCAGTGTCTGTTCCTGAGTAGGGGCAGGGGAAATAGGAGCAGAGTTGCAAAAGAGGCTGAGGAGGGCATG ACTTCATCACTTTGGGGTGAGAGGACCAGCTAGATGCTTGGGCATTTATGGTAGTTATTTTATATCATTT GATTAATAAAAATATTGGAAAATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5151924 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5151924 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14195 same embryo
	EMAGE:14200 same embryo
	EMAGE:14199 same embryo
	EMAGE:14198 same embryo
	EMAGE:14196 same embryo
	EurExpress:euxassay_003998 same experiment
	MGI:4827849 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source