EMAGE mouse gene expression spatial database

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EMAGE:14205

Atp6ap2 ATPase, H+ transporting, lysosomal accessory protein 2 ( MGI:1917745)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004008. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004008_01	euxassay_004008_02	euxassay_004008_03	euxassay_004008_04

euxassay_004008_05	euxassay_004008_06	euxassay_004008_07	euxassay_004008_08	euxassay_004008_09

euxassay_004008_10	euxassay_004008_11	euxassay_004008_12	euxassay_004008_13	euxassay_004008_14

euxassay_004008_15	euxassay_004008_16	euxassay_004008_17	euxassay_004008_18	euxassay_004008_19

euxassay_004008_20	euxassay_004008_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14205_wholemount_strong.wlz

14205_wholemount_moderate.wlz

14205_wholemount_weak.wlz

14205_wholemount_possible.wlz

14205_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14205_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4093
Entity Detected:	Atp6ap2, ATPase, H+ transporting, lysosomal accessory protein 2 ( MGI:1917745)
Sequence:	sense strand is shown >T4093 GTGGCACCATGGCTGTGCTGGTCGTTCTCCTGTTCTTTCTGGTGGCGGGTGCTTTAGGGAATGAATTTAG CATATTAAGATCGCCAGGGTCTGTTGTTTTCCGAAATGGAAATTGGCCTATACCAGGAGATCGAATCCCA GACGTAGCTGCACTGTCCATGGGCTTCTCTGTAAAGGAAGACCTGTCTTGGCCAGGGCTTGCCGTGGGTA ACCTATTCCACCGGCCACGGGCTACCATTATGGTGATGGTGAAGGGGGTGGATAAACTGGCACTTCCGGC AGGCAGCGTCATCTCCTACCCTTTGGAGAATGCAGTTCCTTTTAGTCTAGACAGCGTTGCAAATTCCATT CACTCCTTATTTTCGGAGGAAACACCTGTAGTTTTGCAGTTGGCTCCCAGCGAGGAGAGAGTGTATATGG TGGGGAAAGCAAACTCGGTGTTTGAAGACCTTTCAGTCACATTGCGGCAGCTCCGTAACCGCCTGTTTCA AGAAAACTCCCTTCTCAACTCTCTTCCCCTCAATTCTCTGAGTAGGAATAATGAGGTTGACCTGCTCTTT CTCTCCGAACTGCAAGTGCTACATGATATTTCCAGTTTGTTGTCTCGTCATAAGCATCTAGCCAAGGACC ATTCACCCGACTTGTATTCATTGGAGCTGGCAGGTTTGGATGAACTTGGGAAGCGTTATGGGGAAGACTC TGAACAGTTCAGGGATGCTTCTAAGATCCTTGTTGATGCTCTCCAAAAGTTTGCAGATGACATGTACAGT CTCTATGGTGGGAACGCAGTGGTAGAGTTAGTGACTGTCAAATCATTCGACACATCCCTTGTGAGGAAGT CAAGGACCATCCTTGAGGCAAAACAAGAGAACACCCAAAGTCCCTATAACCTTGCATATAAGTATAATTT GGAGTATTCAGTGGTTTTCAACTTGGTACTGTGGATTATGATCGGCTTGGCCTTGGCTGTGATAATCACC TCTTACAACATTTGGAATATGGATCCTGGATATGATAGCATCATCTATAGGATGACAAATCAGAAGATTC GAATAGATTGAATTTTCCTTATGCTAGACTTAGACAACAGGTTTGGTAATTGGCTGTTTTGTTAAAGTAT ATCTTTTAGTGTTGTTTAAAGTAGACGGTATACCTTAAGTTTATTAAAAAGAAAATATAGATTTCATTAT CTATTTTGTGTGTGCCTATTGTTTTTTTAGAGTGATTTATAGTGTTGACAAGTTGAATTGTGATATAGAT TCAATGTGCTCAAATATTGTTGACATAGCCATTTAATAATGTAATTTCATTCCATTCTATACATGTGGAA ATACGCACTGAGAGAACTGTAAACCACTTAGTCATGTTACTCGCGCTGGAGAACGCACTGGGTTTCTTAG AGAACCTTAAAATGCTTGACTTCTTGCACAGGCTATGTAGTGCAGCTGTTAGTCACTATGAACTATTTGT AAATGTCTTGATCTGCTGTAAATATCTCTGAGACAGGAGGAAATATGTTAACTAAGAGCAGCCCAAAAAT ACTGGATATGCTTATCCAATTGCTTAGTTCTGGACGTGTATATGATTAGCAAGCTAGCTGTTTTTAATCT CTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATATTATGTTGATTT CAGAAGAGACTAGCTTTGTGGAATATAGATGCTTGTAATTAGGTACATAAAAACAAATCTGTGGGGGATA TCTTGAAGCATGAATATAAAATATTTTTATTTCTGTACTTTCCCCCTGTGAAGTTACTCTTGGTTTGTGT GTGGTATGGCTTCAATCTGTAGACTATTATATGGAAGTGGATGATTGCTACTGTTCATGTGCAAATGGAC CAATATCTATATAGAATGACAAATAAAGATAATGATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4457079 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4457079 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14203 same embryo
	EMAGE:14202 same embryo
	EMAGE:14204 same embryo
	EMAGE:14201 same embryo
	EurExpress:euxassay_004008 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source