EMAGE mouse gene expression spatial database

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EMAGE:14424

Prps2 phosphoribosyl pyrophosphate synthetase 2 ( MGI:97776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004232. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004232_01	euxassay_004232_02	euxassay_004232_03	euxassay_004232_04

euxassay_004232_05	euxassay_004232_06	euxassay_004232_07	euxassay_004232_08	euxassay_004232_09

euxassay_004232_10	euxassay_004232_11	euxassay_004232_12	euxassay_004232_13	euxassay_004232_14

euxassay_004232_15	euxassay_004232_16	euxassay_004232_17	euxassay_004232_18	euxassay_004232_19

euxassay_004232_20	euxassay_004232_21	euxassay_004232_22	euxassay_004232_23	euxassay_004232_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14424_wholemount_strong.wlz

14424_wholemount_moderate.wlz

14424_wholemount_weak.wlz

14424_wholemount_possible.wlz

14424_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14424_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7155
Entity Detected:	Prps2, phosphoribosyl pyrophosphate synthetase 2 ( MGI:97776)
Sequence:	sense strand is shown >T7155 GCAGCAGCAGCAGCAACAGCCGCAGCAACGGTAGCTGTAGTCTGCATCGCAGTCCCTTTCTCCTTCTCCA GCGCGCTCCTCAGTCCCCGGTCACCATGCCTAACATCGTGCTCTTCAGCGGCAGTTCTCACCAGGACCTG TCGCAGCGCGTGGCCGATCGCCTGGGCCTGGAGCTGGGCAAGGTGGTCACCAAGAAGTTTAGCAACCAGG AGACCAGTGTCGAGATCGGTGAAAGTGTGAGAGGGGAAGACGTCTACATCATCCAGAGTGGCTGCGGGGA GATTAATGACAACCTGATGGAGCTGCTCATCATGATCAATGCGTGCAAGATTGCGTCCTCATCCAGGGTG ACAGCTGTGATACCCTGCTTCCCCTACGCCCGGCAAGATAAAAAGGACAAGAGTCGTGCTCCAATTTCTG CTAAACTTGTGGCCAATATGCTGTCAGTTGCTGGGGCTGATCACATCATTACCATGGACCTGCATGCCTC TCAGATCCAGGGTTTCTTTGATATTCCTGTGGATAATTTATATGCAGAACCTGCAGTTCTTCAGTGGATT CGGGAGAACATCACTGAATGGAGAAACTGTATTATTGTTTCTCCAGATGCTGGAGGAGCCAAAAGGGTTA CATCAATTGCAGACAGACTGAATGTGGAATTTGCTTTGATTCACAAAGAGAGAAAGAAAGCAAATGAAGT GGACCGGATGGTTCTCGTGGGAGATGTGAAGGACCGTGTGGCCATCCTGGTAGACGACATGGCCGATACA TGTGGTACCATCTGCCATGCTGCAGATAAGTTACTCTCAGCTGGTGCCACCAAAGTTTATGCTATTCTTA CCCATGGAATCTTCTCTGGGCCAGCTATTTCCAGAATAAACAGTGCTGCCTTTGAAGCTGTTGTTGTCAC CAACACAATTCCACAAGAGGACAAAATGAAACACTGCTCCAAAATTCAGGTGATTGACATTTCGATGATC CTGGCTGAAGCCATTCGAAGAACCCACAATGGGGAATCCGTGTCCTATCTGTTCAGCCATGTCCCACTGT AGTCTGGATTGGAATATTGAAGATGGAGCAAGCCTCCTCCAACTTCTCACTGTCTTTGTCTGCCTGCTGT TTTAGAATTAGGACTCCGCAATTCTAGGATGATCACTGGCAAAAGCATCTGATCTGTGTCCACTCTAAGA GCCGTCTGTCTCTCCTTCTAAAGCTCCAGTCCTGGGGGAGGGCAGAGAAAGGTGGTGGTTATTTGGTCGT TAATTGAACTGGTGTCAATGAGAACTAATCTTTTCCCTCGTTCTTTTTTAGTACTTTGAGGTAACAACTT TTAAGCCTAAAATCCCATTGTGGAAATTTGTCATACATACATTTTGAGGATGCCAAAGGTGATTTCAAAT GAAAGCCTTCTGGGTAAATATATAATAATGCCTGTGGATATTTGGGGGGAAATCCTAAATAACCTTTATG TCAGAGAGAACCTTGGAAAATTTATACTATCATGAATTTTGAAATTTTGTTGTTTTTTTCTTAATGATAA CTGGGTTTATTTTATTGCCCTTTATCAGGACTGAATTTTATTGGGCCTAAGATAGCCACTGACATACTGA CTTTTCTTAGTAGATATTAGACATATTAAAGACAATATAAGGCTGTATCATTTTTTAATTGCCACTTTGA AGATATATCATAAATGACAACTTACCGGAAACTCCCAAATTATGATCAGTGATCACTGATTTGGCTACCA TTTCAGGCTGTAGTCAATGAGTTTGTGACCACGTTGATGCACAACTTTGTCTATCCCAGGGGAATTTCTC TTTAGAATGTAGCTCATGTCCTATCACCTTATTCTCAGACGTTAACAATAGCCTTTACTTTCCACATTCC CCAATAGTTTCTTGTTGTAGATGTCCACCAAAAGAAAGGCTCCTTGAAACCCCCTCACATATTTTAGGTT GATTATTATTTTATTTTTTCACATACATAAAATGGGGAGGATTTAAGAGTGCCACCAAATACACTTATTC AAACAGAGCTTACTGAGCTTGAGTTAAATGTCCTTTGGCCCTTTGTCATCAGAAGAACTCCATCTCTAAT GACTAGTTAGCATCTCTAAAGCTGCTAAAATGTAGACAGGAGAGGGTTGCAAAACAGTGGAGGCAAAAGT AAATAGCTGTTCCATTAAAATAAACTTCAAAGAAATGCTTCAGAAAGCATTGTGAAGAAGAATCTCATTG TGGGTGGCATTCTTGATAAAATGATCTTTTAGTGAAGTGTGGAGGTCTGTACCCGTAATCCCAGCACTTT AAAACTAATGCAGGAAGAGTACCACAAGTTTAAGGCCAGCCTAGGCTACACAGTGAGTTCCAGCTCAGCT TGGCCTGGAGAGCTGAGACTGTGTGTCATAAAACACAAACAAACAAAAAGCATCAGTAAGTGCACAACCT ATCATTTTCCTTCTCACAAAGAAGAGGATTTGCCTGTGTACCTAAAGCTTAGGAATGCTTAAAAAAAATC CCCCCCTTGCTGAGTATTGTAGATAAATTCCCAGCCCTAAATTTTGCAGGTAAGAAATTCCTATCCATCT GAGTAGCACACTCAACCAGCACTCTGGAACAGCAGAGGAGCAAAAGTCAAATCAATGTTACAAAGTATAA TTTATGTTCCTATAGAGACTGGCATTTTGATAATACCTTATTTGTAACTGTTCTGAAGAAAGTGGTTCCT ATAACCTGGGATGCTCAGCTCTGTGGTACAGTCTGTCTCAGGCTTGGTTAGACATGTGTCAAAACTTGTT TGAGATGATGCAGCTGTTACTTCTGATAAATGTGCGATGTCTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4022452 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4022452 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14426 same embryo
	EMAGE:14429 same embryo
	EMAGE:14427 same embryo
	EMAGE:14425 same embryo
	EMAGE:14428 same embryo
	EurExpress:euxassay_004232 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source