EMAGE mouse gene expression spatial database

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EMAGE:14425

Nkd2 naked cuticle 2 homolog (Drosophila) ( MGI:1919543)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004230. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004230_01	euxassay_004230_02	euxassay_004230_03	euxassay_004230_04

euxassay_004230_05	euxassay_004230_06	euxassay_004230_07	euxassay_004230_08	euxassay_004230_09

euxassay_004230_10	euxassay_004230_11	euxassay_004230_12	euxassay_004230_13	euxassay_004230_14

euxassay_004230_15	euxassay_004230_16	euxassay_004230_17	euxassay_004230_18	euxassay_004230_19

euxassay_004230_20	euxassay_004230_21	euxassay_004230_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14425_wholemount_strong.wlz

14425_wholemount_moderate.wlz

14425_wholemount_weak.wlz

14425_wholemount_possible.wlz

14425_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14425_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 12 18 19

vibrissa

moderate

regional

moderate expression: see section 08 09 10 weak expression: see section 11 21 22

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 14 15 16

mandible

weak

regional

weak expression: see section 07 08 09 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7153
Entity Detected:	Nkd2, naked cuticle 2 homolog (Drosophila) ( MGI:1919543)
Sequence:	sense strand is shown >T7153 GCCGCTGCCCGCGCCGGTGTCTCCGGTTGCCGGGCCAAGCCTCGCCAGACTGCCCGGCCCCGGATCCAGG CTCCACGAGTTGCTCTCAGCTCGGGATTCACGCACCCCGGGCTCGAACCTCGGACGCACACCTGAGAGCT CGGCCGGAGGGTGGGTCCAGGCCCAGCGGAGACATGGGGAAATTTCAGTCCAAGCACGCCGCAGCAGCCT GCAAGAGGAGAGAGAGTCCCGAAGGGGACAGCTTCGTGGTGCCTGCGTACGGGAGCGGTCGCAGAGGAGC GGAAGAAACGGACCGGCGTGCGGGCAGCGGTGTGGAACATCGCTCACGGGACAAGCAGGAGCTGCTCAAT GGAGACCCTAAGGAGGGGCCTTTCTGGGACGACAAGGGTTCCCTAGAAGTTGCGCTGCCCCCTGAGAAGT CTGAGGGCCATGAGGGCCAGGGCCAGCTCTTCAGCACAGATGATGGGGAGAAGGCAGCAAGCCGTGAGGG TCCACTGAGACTTAGCAAGAAGCACTTGAACATTGACGCACTGCAGTGTGACGTCTCAGTGGAAGAAGAC AACCGCCAAGAATGGACATTCACGTTGTATGACTTTGACAACAGTGGGAAAGTCACCAGAGAGGATATGT CCAGCCTGATGCACACCATCTACGAGGTTGTCGATGCCTCTGTCAATCACTCCTCCGGCAGCAGCAAGAC CCTTCGAGTGAAGCTAACTGTCAGCCCTGAACCCTCCAGCAAGAAGGAATGTCCTCTCACTGGCCAAGAC CGGGAGCCCACTCGTGGCAGAACAGAGATTGAGCTCACAGATGAGCCCCGAGCGGCTGACAGAAGGCTAT CCGCCTACAGCAGGAAGCCCAATGCTGATCCCCAGCCCTGCTCTGTGCGAGTGCCCTACTGTGTGGATGA GAACACAGAGCGCAGAAACCACTACCTAGACCTTGCTGGCATCGAGAACTACACATCTAAGTTTGGTCCT GGGTCACCACCTGAGCAGGCCAGGCAAGAACATCATGGCAGGGCCACACACATTCCAAGCAGGTCCCGAT CACAAGAGTCGGATGCCCACGCTATACACCACCGCAGGTCTCAAGTCCTGGCTGAGCATGTCATACCAGC TAATGAGCCTGCCACCCGGGCCCTGGCTGCACAGCCCCGGCTCAAGGGGCAGGAGAAGCAGTTCCTCAGG TCTCCTAAGGGTCCAGGAAAACCTCTTGGGACACCAGGCAGTGGCAAGCCAGGGAAAGCTCTCAGCTATT GCCTGCAGGCTGTGCCACTGCCCCAGGGTGCTCAGGATGGCCACCACCTTCCTCAGCCCCCACCACAGCC TCCACCGCAGCCCTATGGTCACAAGCGGTACCGGCAGAAAGCCAGAGAAGGCCACTCACCACTTAAGGGG CATGGCCAGCCTACCATGGTGGAGCATGAAGTGGTACGGGACCTGCCTCCCATGCTGGGGCCTGAGGGCT ATGTGATGCCTGTGGTCCAGAGGCATGAACACCACCATCACCATGAGCACCACCACCATCACCACCACCA CCACTTCCACCCATCCTAGCACCCAAACAAGCACTCTGCCCAAATGCCCTTGGGAACAGAGCAGGGGCTG GCATGTGCCTAGTAGAGAATACAGCATCCCCTGGCTCAACACCAGCTACAGATGTCAAGGGGGCACACCA GGGAGATGGCACCAGGCTTGGATATTCATGGACAAAGCTACCTTTCTCCATGCCATTTGGATACTAGTAC ATTTGATGCAGCTGATGGTTACCAGGGACACGTGCTCCTGTGTCATCCAGAAAGCCCATTCATGAGGGCA CAAAAGCATCTGTTCCTGCTTTTGCTGCAGTGTGGCCATGGGATATTTGAAGGCATTCTTTGTATCCTCA TGGTTCTTTGACCTTCGGGTACCTGAATAGAAAAGGCAATGCTTTTTGTGAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4021772 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4021772 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14426 same embryo
	EMAGE:14429 same embryo
	EMAGE:14427 same embryo
	EMAGE:14428 same embryo
	EMAGE:14424 same embryo
	EurExpress:euxassay_004230 same experiment
	MGI:4826718 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source