EMAGE mouse gene expression spatial database

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EMAGE:14438

Taf12 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1913714)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004258. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004258_01	euxassay_004258_02	euxassay_004258_03	euxassay_004258_04

euxassay_004258_05	euxassay_004258_06	euxassay_004258_07	euxassay_004258_08	euxassay_004258_09

euxassay_004258_10	euxassay_004258_11	euxassay_004258_12	euxassay_004258_13	euxassay_004258_14

euxassay_004258_15	euxassay_004258_16	euxassay_004258_17	euxassay_004258_18	euxassay_004258_19

euxassay_004258_20	euxassay_004258_21	euxassay_004258_22	euxassay_004258_23	euxassay_004258_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14438_wholemount_strong.wlz

14438_wholemount_moderate.wlz

14438_wholemount_weak.wlz

14438_wholemount_possible.wlz

14438_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14438_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6095
Entity Detected:	Taf12, TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1913714)
Sequence:	sense strand is shown >T6095 GACGAAAGCTTCACTGGGGCAGTCTCCGCATATCATGGGGAGATAGACGCTGCTGCCTCTGATTGGCCTT GGTCCTCACAGCTCTAAAAAGAAGCAGGATCTTGACAAGCTCTATGAGTTGAAGTCTAAAGCTCGGCAGA TTATGAACCAGTTTGGCCCTTCAGCGCTAATCAACCTCTCCAGTTTCTCATCTGTAAAACCAGAGCCAGC TAGCACCCCGCCACAAGGCTCCATGGCCAATAGCACCACCGTGGGGAAGATAGCAGGCACTCCTGGGACA GGTGGTCGTCTCAGCCCTGAAAACAACCAGGTATTGACCAAGAAGAAATTACAAGACCTAGTAAGAGAAG TAGATCCTAATGAGCAGCTGGATGAAGATGTGGAAGAGATGCTGCTACAGATCGCTGATGATTTTATTGA GAGTGTGGTGACAGCTGCCTGCCAGCTTGCTCGGCACCGCAAGTCCAGCACCCTAGAGGTAAAAGACGTC CAGCTGCATCTAGAGCGCCAGTGGAACATGTGGATCCCAGGCTTTGGCTCTGAGGAAATCCGACCCTACA AAAAAGCATGTACCACAGAGGCTCACAAACAGAGAATGGCACTGATCCGGAAAACAACCAAGAAATAACA CAGGGAACGGTCAGGGAATGGACAACGTGTATTTGGAGATACTTGAGCTGAGACCTCAGCCATCTCATCC TTGGATTTTTTTTTAAATGCTTTACAGAGAAGCATATATTTTTTATTAACAGTGCAGCAATATCTATAAT GACTGAGAAGATCTGCCAAAAGAATAAAGCCTCCTGCCCACCTTCTGGTTCCTTTTCCCTGCCGTCTCAA AGAGAAGCAGTGTATCTACCAGAGAAGATTTTGCCTGTGGTTTATTATAGAAGTGATTGGCTGTAGGAAG AGCATTTTGGTCGTGTTGGGTGAGACAGTGTTAGCAAGTTTGTAGAGGGTCTGTCTCCTCCTCCCCCCGT CCCTGTACTTTGTAATGTCAGTGTTTCTATGAATATGAACTTGATTTGCTTTTCCAGAATAAAGGAGTTA TTAATTGAAGACACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3493047 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493047 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14442 same embryo
	EMAGE:14441 same embryo
	EMAGE:14439 same embryo
	EMAGE:14437 same embryo
	EMAGE:14440 same embryo
	EurExpress:euxassay_004258 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source