EMAGE mouse gene expression spatial database

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EMAGE:14549

Nup155 nucleoporin 155 ( MGI:2181182)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005420. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005420_01	euxassay_005420_02	euxassay_005420_03	euxassay_005420_04

euxassay_005420_05	euxassay_005420_06	euxassay_005420_07	euxassay_005420_08	euxassay_005420_09

euxassay_005420_10	euxassay_005420_11	euxassay_005420_12	euxassay_005420_13	euxassay_005420_14

euxassay_005420_15	euxassay_005420_16	euxassay_005420_17	euxassay_005420_18	euxassay_005420_19

euxassay_005420_20	euxassay_005420_21	euxassay_005420_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14549_wholemount_strong.wlz

14549_wholemount_moderate.wlz

14549_wholemount_weak.wlz

14549_wholemount_possible.wlz

14549_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14549_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8768
Entity Detected:	Nup155, nucleoporin 155 ( MGI:2181182)
Sequence:	sense strand is shown >T8768 GGAGCGGTTTTGGGCTCTCTTCGTTTTTCTCACGGTTCTCCACGGGTTTGGAGTGTTTCTATAAGGCGGT GGCCACGATTGCCGACATGCCATCGGTGCTGGGCTCTATGATGGTGGCCTCTACGTCGGCCGCCGCCTCC CTGCAGGAAGCTTTGGAAAACGCGGGGAGGCTTATCGATCGACAGTTGCAAGAAGACCGCATGTACCCGG ACCTTTCTGAGCTGCTCATGGTGTCGGCCCCAAACAGTCCTACTGTTTCTGGAATGTCCGACATGGATTA CCCTCTGCAAGGACCAGGTTTGCTGTCAGTACCCAGCCTTCCAGAGATCAGCACTATCCGGAGAGTTCCC CTCCCGCCTGAGCTGGTTGAACAGTTTGGACACATGCAGTGCAACTGCATGATGGGCGTTTTCCCCCCGA TCAGCAGAGCCTGGCTTACGATTGACAGTGACATATTCATGTGGAACTATGAAGATGGAGGTGACCTTGC CTATTTTGATGGACTCAGTGAGACAATTCTTGCTGTGGGGCTTGTGAAACCAAAAGCTGGCATCTTTCAA CCTCATGTAAGACACTTGCTGGTTTTGGCAACCCCTGTGGATATAGTAATTCTTGGACTCAGCTATGCTA ATGTACAAACAGGTTCTGGCATTCTCAATGACAGCATGTGTGGCGGCATGCAGCTGCTTCCAGATCCCTT ATATTCTCTTCCCACGGACAACACGTACCTTTTAACAATCACGTCCACGGATAATGGCAGAATCTTTCTG GCTGGAAAGGATGGCTGTTTGTACGAAGTAGCATACCAAGCAGAAGCAGGCTGGTTCAGTCAAAGATGTA GGAAGATCAATCACTCCAAGAGCTCGCTCTCCTTCCTGGTGCCTTCCTTGCTGCAGTTCACGTTCTCAGA AGATGATCCTATTGTTCAAATTGAAATTGATAATTCCAGAAATATTTTGTATACACGATCGGAGAAAGGA GTTATACAGGTTTATGATTTGGGCCATGATGGACAGGGGATGAGTAGAGTTGCTTCAGTTTCACAGAATG CCATTGTCTCTGCTGCTGGAAACATTGCTAGGACTATTGATCGTTCCGTCTTTAAGCCAATTGTTCAGAT AGCTGTGATTGAGAGTTCTGAATCCCTGGACTGTCAGTTACTGGCTGTCACTCACGCAGGTGTAAGGCTG TATTTCAGCACTTGTCCGTTTAGACAGCCATTAGCTCGGCCGAATACACTGACACTGGTCCACGTCCGGT TACCTCCTGGATTTTCAGCATCATCAACAGTGGAAAAGCCTTCAAAAGTGCACAAAGCACTTTACAGTAA AGGAATTTTACTGATGACAGCCTCAGAAAATGAAGATAATGACATTTTATGGTGTGTCAACCATGATACA TTTCCTTTTCAAAAGCCAATGATGGAAACTCAGATGACAACCCGTGTTGATGGTCATTCCTGGGCTCTCT CTGCAATAGATGAATTAAAAGTAGATAAAATAATTACACCGTTAAATAAAGATCATATTCCAATAACTGA TTCGCCAGTTGTTGTACAACAGCACATGTTGCCTCCAAAGAAATTCGTTCTCCTCTCAGCACAGGGTAGT CTTATGTTTCACAAGCTTAGACCTGTAGATCAGCTGAGACATCTGCTTGTGAGTAACGTGGGAGGGGATG GAGAAGAGATTGAGAGATTCTTCAAATTACATCAGGAAGACCAAGCTTGTGCAACTTGCCTTATTCTTGC TTGTTCCACTGCTGCCTGTGACAGAGAAGTGTCTGCTTGGGCAACACGGGCATTCTTTAGGTATGGTGGT GAGGCACAGATGAGATTTCCAGCTACTCTTCCTACTCCAAGCAACGTTGGTCCCATCCTTGGCTCTCCTA TGTATTCTAGTTCTCCTGTCCCTAGTGGGAGTCCCTATCCAAATCCATCCTCCTTGGGGACACCATCCCA TGGTGCTCAGCCGCCTACCATGTCAACCCCAATGTGTGCTGTTGGAAGCCCAGCCATGCAGGCTGCAAGC ATGAGTGGTTTGACTGGGCCAGAGATTGTGTACTCAGGAAAACACAACGGTATTTGCATTTACTTTTCTC GAATCATGGGAAATATTTGGGATGCGAGCTTAGTTGTTGAAAGAGTATTCAAGAGTTCCAACAGGGAGAT CACTGCAATTGAGAGCAGTGTGCCCGTCCAGCTGCTGGAGTCAGTGCTGCAGGAGCTGAAGGGCTTGCAG GAGTTTCTAGACAGAAACTCCCAGTTTTCAGGTGGACCCCTGGGAAATCCAAATACTACTGCCAGAGTGC AGCAGCGGCTGGTTGGGTTCATGCGTCCCGAGAATGGAAACACCCAGCAAATGCAACAGGAGCTGCAGAG GAAGTTTCAGGAGGCTCAACTGAGTGAAAAGATTTCACTTCAGGCGATCCAGCAGCTGGTTAGAAAATCG TACCAAGCTCTGGCTCTCTGGAAACTCCTTTGTGAACATCAGTTTTCTGTCATTGTCGGAGAACTTCAAA AGGAATTTCAAGAGCAGCTGAAGATCACCACCTTTAAGGATCTGGTGATCAGGGACAAAGAGGTCACTGG AGCATTAATTGCTTCTCTTATCAACTGCTACATCAGAGACAACGCCGCCGTAGACGGCATTAGCTTACAT CTGCAGGACACCTGTCCGCTTCTGTATAGCACAGACGATGCAGTTTGTTCTAAGGCAAATGAGCTCCTTC AGCGTTCCCGACAAGTTCAGAGTAAGACTGAGAGAGAGAGGATGTTACGGGAGTCACTGAAGGAGTATCA GAAGATCAGCAACCAAGTGGACCTTCCCAGTGTCTGTGCTCAGTATAGGCAAGTGCGTTTTTATGAGGGT GTGGTGGAACTTTCTCTTACTGCTGCCGAGAAAAAAGATCCTCAGGGTCTTGGACTCCATTTCTATAAAC ATGGGGAACCAGAAGAGGATGTGGTTGGTCTTCAGACTTTCCAAGAGAGATTAAACAGTTACAAGTGCAT TACGGATACTCTGCAAGAGCTGGTAAATCAAAGTAAGGCAGCTCCCCAGTCTCCCAGTGTACCCAAAAAG CCTGGGCCTCCAGTGCTGTCATCTGATCCCAACATGCTGAGCAATGAAGAAGCAGGACATCACTTTGAAC AAATGCTTAAATTGGCACAGCGGTCCAAGGATGAGCTCTTCAGCATTGCTCTTTATAACTGGCTAATACA AGCTGACCTTGCAGATAAACTGCTACAGATTGCGTCACCATTTCTGGAGCCACATCTTGTGCGAATGGCC AGAGTGGATCAGAACAGAGTCCGCTATATGGATTTACTCTGGAGGTATTACGAGAAGAACAGGAGCTTCA GCAGCGCCGCTCGGGTCCTATCCAAGTTGGCGGACATGCACAGCACAGAAATTTCACTTCAGCAGCGACT AGAATACATTGCTCGAGCCATCCTTAGTGCGAAAAGTTCCACTGCCATCTCATCCATAGCTGCAGATGGT GAATTCCTGCATGAGTTAGAAGAGAAAATGGAAGTTGCTAGAATCCAACTTCAGATACAAGAGACACTAC AAAGGCAGTACTCCCATCATTCTTCAGTGCAGGATGCAATTTCTCAGCTGGATTCTGAGCTCATGGACAT TACTAAGCTCTATGGGGAATTTGCTGACCCGTTTAAACTTGCAGAGAGTAAACTTGCAGTCATTCATTGC GCTGGTTACTCAGATCCCATATTGGTGCACACACTCTGGCAAGATATCATAGAGAAAGAATTGAACGACA GCGTAGCATTGAGCTCGTCAGATCGAATGCATGCTCTCAGCCTCAAGCTTGTCCTCCTCGGCAAAATCTA TGCTGGGACGCCTCGGTTTTTCCCTCTGGATTTTATCGTGCAGTTCTTGGAGCAGCAAGTTTGTACCTTA AACTGGGATGTGGGGTTTGTGATACAGACCATGAATGAAATCGGCGTGCCTTTACCTAGGCTGCTAGAAG TTTATGATCAGCTCTTCAAATCACGGGATCCATTCTGGAACAGAGTGAAAAGCCCACTGCACCTTCTGGA TTGTATCCATGTACTGTTGACAAGATATGTTGAGAATCCTAGCCTAGTTTTAAATTGTGAGAGGAGAAGG TTTACAAATCTCTGCCTGGATGCCGTCTGTGGATATCTGGTGGAACTTCAGTCAATGAGTTCTTCAGTAG CAGTACAAGCCATCACTGGGAATTTTAAATCGCTCCAAGCAAAGCTGGAACGGCTCCATTGATTACTGTA GTATATTTTCTTGTTTGCATTTTGGTCTGTAAAATAAAAGCTCAGCTGGGTCCAGAAATCAGGTGTTGTA TATCTAGAATTTTGGTAGAACATTTTTTTAATGAATGATAGTTATCTACAAATAGTACATTTGCCAAATG ATTTAGTTTTTTTTTTTATGATTAGTATTTTTTTTAATTGATAAGTTGGGCCCAGATATGTAGCTCAGTG GTAAAATGTTTGTATAGTGTGTAAAAAGTTAGTAGTACCACACACAGACACAGCAGTCTATGAACAGCTG GAATAAGACTGTGTATGATTATGACACACAAAGATTTTGAATTTCACTAGTGGTAACAATTTTGACTTTG TATTATTTATCTACAGGTTTATGTAGGATGTTAGTATTAAAACTTATAGGCTATGCCAGGTTTGGTAGCA CATGCCTTTAAGTCCAGCACTTGGGGAAGCAGTGGCAGGCGGCTCTTTAGAACTCACTGAGATTTCCAGG CCAGGGGCTACACAGTGAGACCTTATCTTCAAACAAACAAACAAACAAACAAACACCTTATAGGCTGGGT CTGTTCCCTTAGTGCTAGATTCTGTAACAGTCTTAATTAGTCATTTTTAGGTTAATATTTTATCAATTAC AGCCCTTTATGTTCTCTCTTCCTCTTTTTTTCTCTCTCCCTCTCACCATAAGTACATACATTAGATTTTA TGTATACATTTATATATGTGTGTGCAGGCTATATATGTATGTTGTATGTATATACAGGATTGTTTGAGCA TGTGATTGTAACACTTCTATGTACTTAAAATCTTCATGATCACCCAGCTGTAATGGTGCTCCCCTTTAAT GGCGGCACTCGGGAGTGTGGGGGAGAGTCAGAGTGGCCAGTCTGGTCTACATACTGAGTTACAATTCAAC CACAGCTCTACAGGGACTACTGTTTCCTTAGAACCCTCACTGTCTAACTGTATGCTTCAAGCCAGCAACA GTAGCAGCAAATTAAATTACAAATTAAATTCCTGGCTCTCTGGTCAGATCTACCAAGCCAAAAAAAAAAA AACAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406277 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406277 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14552 same embryo
	EMAGE:14548 same embryo
	EMAGE:14550 same embryo
	EMAGE:14551 same embryo
	EMAGE:14547 same embryo
	EurExpress:euxassay_005420 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source