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EMAGE:14606

Ubl4b ubiquitin-like 4B ( MGI:1914841)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606
"Pseudo-wholemount" of euxassay_005458. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005458_01 euxassay_005458_02 euxassay_005458_03 euxassay_005458_04
EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606
euxassay_005458_05 euxassay_005458_06 euxassay_005458_07 euxassay_005458_08 euxassay_005458_09
EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606
euxassay_005458_10 euxassay_005458_11 euxassay_005458_12 euxassay_005458_13 euxassay_005458_14
EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606 EMAGE:14606
euxassay_005458_15 euxassay_005458_16 euxassay_005458_17 euxassay_005458_18 euxassay_005458_19
EMAGE:14606 EMAGE:14606 EMAGE:14606
euxassay_005458_20 euxassay_005458_21 euxassay_005458_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14606Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14606_wholemount_strong.wlz
14606_wholemount_moderate.wlz
14606_wholemount_weak.wlz
14606_wholemount_possible.wlz
14606_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14606_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9894
Entity Detected:Ubl4b, ubiquitin-like 4B ( MGI:1914841)
Sequence:sense strand is shown

>T9894
GCCCTACTAGGAGCTGTGACAGAGTCACCTATTGGGCCCTACACCTTCAGGGCATCTCCCAGAGCATGGA
TCCCTCCTGATTCCTCTCTGGCCCCAATGTTCCTCACGGTCAAGCTGCTCTTGGGCCGAAGATGCAGCCT
GAAGGTATCTGGAAAAGAAAGTGTGGCCACATTGAAGAAACTGGTTTCTCAGCACCTACAGGTGCCCGAG
GAGCAGCAGCACCTGCTGTTCCGAGGCCAGCTCCTGGCTGATGACAAATACCTCTCAGATTACAGCATTG
GGCCCAACGCTTCCATCAATGTCATCATGCGGCCTCCAGAGGATGCAGCGCTGGATAAGACCCATCAAAC
CCAGCCCCTGTGGCTCCAGCTGGGCCAGGTTCTAGACAAACATTTTGGAGCAAAGGATGCAAAGACAGTG
CTGGGGTTTTTGAGGCAAGAGCATGAGGAGCGTCTGCAGAGGCTGAGCCTGGAGGCCCTGGAGCAGTTGG
TGGGCCAGCTCCTAGCACAACAGCAGCTTGACGAGCTGGCAGAGGAAAAGGAAGCCCCTGCTGTTGCATC
GGAACTCGAGCAAAACAATGGAGGAGGAGGAGGAGGAGGAGGGACAGGAGGAGAAGGAGGAGGAAAGAAG
GAAGAAGAGGAGGGTGAGGAGGCTGATCAGTAAATTGGCCTGTCCTTCTCTTGCTCACTAAACTTCCCCT
GGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6774419 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6774419 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14607 same embryo
 EMAGE:14609 same embryo
 EMAGE:14605 same embryo
 EMAGE:14608 same embryo
 EMAGE:14604 same embryo
 EurExpress:euxassay_005458 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS