EMAGE mouse gene expression spatial database

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EMAGE:14640

Narg2 NMDA receptor-regulated gene 2 ( MGI:2135947)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005568. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005568_01	euxassay_005568_02	euxassay_005568_03	euxassay_005568_04

euxassay_005568_05	euxassay_005568_06	euxassay_005568_07	euxassay_005568_08	euxassay_005568_09

euxassay_005568_10	euxassay_005568_11	euxassay_005568_12	euxassay_005568_13	euxassay_005568_14

euxassay_005568_15	euxassay_005568_16	euxassay_005568_17	euxassay_005568_18	euxassay_005568_19

euxassay_005568_20	euxassay_005568_21	euxassay_005568_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14640_wholemount_strong.wlz

14640_wholemount_moderate.wlz

14640_wholemount_weak.wlz

14640_wholemount_possible.wlz

14640_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14640_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9598
Entity Detected:	Narg2, NMDA receptor-regulated gene 2 ( MGI:2135947)
Sequence:	sense strand is shown >T9598 AACGTGTGTGGAAGCGTCGGCTTTTCTCGGGGGCCAGAGCCGCTTGGGACCCTGAGTTAACTGTAGACTC TAGGAAGGATCCGACCCTCACTCTCTGGGAGCCTTCACTGCCCAGCTGAAATAATTGTGGGCTAGAGTGA AGGAGAATTCTAGGAAGATGAGCTCTAGCATAACCATGAGTGAACCAAGGCTGAACTGGGATGTTACACC CAAAAATGGCCTTAAAGCATTTTTCTCTCCAGAAAATTATAAAGACCATTCCATGGCTCCAAGTTTAAAA GAACTCTATATTTTGTCTAACAGACGTATAGGAGAAAATTTGAGTGTCTCAGCAAGTTCTGTAGAAAATG AGCCTGCAGTTAGTTCAGCAACTCAAGCAAAGGAAAAAGTTGGGATGATTCTCCTTCCAAAACCACGAGT TCCTTATCCTCGTTTCTCTCGGTTCTCACAGAGGGAGCAGAGAACTTATGTGGATTTGTTGGCTAAATAT GCAAAGCTCCCCTCAAGTTCCAAGACTGTCGGAACAAATACCAATGAATATCTGCAGTATCTGGATATGA AAAAACATGTGAATGAAGAAGTTAATGAATTTCTAAAGTTTTTACAAAATTCTGCAAAGAAATGTGCACA GGATTATAATATGCTCTCTGATGAGGCCCGACTCTTCACGGAGCAACTCCTAAGAGCTTGCATTGAACAA GTGAAAAAGTACCCAGAATTTTATACTCTTCATGAAGTTACCAGCCTAATGGGATTCTTCCCCTTCAAAA CAGAGATGGGACTGAAGTTGGAAAAGACTCTTCTTGTTTTGGGCAGTGCAAAGTTTGTAAAAACAGCATT TCCCTCAATGCCTGTGAAATTACAGCTCTCAAAAGAGGACATGTCTTCCATTGAGACCCCACAACAAAAA GCTGAAGTTATGCACTGTGATATTAGTAAAGACCCGAACGCAGAGAAGCTTGTTTCAAGATACCACCCGC AGATAGCTCTGACTAGCCAAGCATTATTCACCTTGCTCAATAACCATGGACCAAGCTACAAGGAGCAGTG GGAAATTCCAGTGTGTGTTGAGATGATAGCTGTGGAAGGTTCCAAACCAGTTAAAGTAATTTATATCAAT TCTCCACTTCCTCGAAAGCAAATGACAATGAGAGAAAGAAATCAAATCTTCCATGAAGTTCCATTAAAAC ATATAATATCAAAAAACACATCTGTTCCAGTCTCTGCTGTCTTTATGGACAAACCTGAAGAGTACACCTC TGAGGTGGACATGCCCACTGAAGCTGGTGAGTGCCGAAAAATTGAAACTCTGGAAAATTTGGATATGGAC TTCGATGGTGATGTCACAGAACTTGAAACCTTTGGAGTAACTACTACTAGTCCACCTAGGTCACCAAGTT CAGAAAGTGACTCCTCTGCACCCCTCATGACAGATGTCCACGCAGTCCCCAAAATAGCAGCTGTGCCTCT GGCACCAGCTACGCCTGTGGCACCCACTATGCCTGTGGCACCAGCTACACCTGTCACACCCACTATGCCC ATGGCACCAGCTACACCTGAGGCATCAGCTACACCTAACATTACTGATGATTCTAGAAGTTTATGTCAGA TTCTGATGAAACAATTACAGAAGGAGAAACAGCTATTCTCTGGTGTGGAAGGTGGTCCTGAAGGGTGCAA AAATAAAGATGATCAAGGACTTGAACCATGTGGTGAAGAGGTCCCCAGTGCCAATGCAAAATCTCTAACA CAAGATAATGAAGTCCACAGAACTGAGGGAATTTCTAAGGAAAGTGATGTAGGTGTGCTGTGTACCAATG ATGAAAGACAGGGTGGTCAGGGGAATGCAAACAATCCCAACAACACAGCTACTGCATCAGAAGCAGCTGA AAGTGAGAAAGGGATTCCTTGTGGCAGTGATACAGATGAGGACTGCTTAATCATTGATACAGAGAGTAGA AGTTGTGATGGGAAGACAGCTGATTTAGGTTCTAGGCCAAATTCTTCAGCACAGGCTTCTGCAGGGAATC AGGCCACTACCACTGTTTCTGAGGAATCTTGTGTTTTAAAGAAACCTATCAAAAGAGTCTACAAAAAATT TGATCCAGTTGGAGAAATTCTAAAAATGCAGGATGAGCTTTTAAAACCAGTTTCCAGGAAAGTACCTGAG TTGCCCTTGACTAATTCAGAAGAATCTAAACAGCCGCCTGCTTCTGAGCAACCCTCTGCTGCTTTGGATG CCGCTCCCTGGCCAAAATCCAGTTGGCCCTCTGCATTTCAGAAGCCTAAAGGACGATTGCCATATGAACT TCAAGACTATGTGGAAGATACATCAGAATATATAGCTCCTCAAGAAGGAAACTTTGTCTACAAGTTGTTC AGCCTACAAGACCTGTTGTTACTTGTGCGGTGCAGCATCCAGAGGGTAGAAACGAGACCACGCTCCAAAA AACGGAAGAAGATCAGAAGACAATTTCCAGTTTACGTGCTACCAAAAGTAGAGTATCAAGGTTGTTATGG AGTTGAAGCTCTGACTGAAAGCGAGCTCTGTCGCTTCTGGACTGAAAGTTTATTGCATTCCAACTGCTCG TTTTATGTTGGGCATATTGATGCATTTACTTCAAAGCTTTTCATGCTAGAAGAAATCGCTTCAGAAGAAT TAAAAGAAAAACTTGCAGCACTCAAGATTTCAAGCTTATTTAACATTCTCCAGCACATTCTAAAGAAATT ATGTAGCTTGCAGGAAGGGTCCTACTTGCTGTCTCATGCAGCAGAAGATTCTTCACTCCTGATCTACAAG ACCTCAGATGGAAAAGTTACTAGAACGGCATATAATTTGCATAAGGCACATTGTGACCTTCCTGGCGTAC CCTCCAGTCTCTCGGTTCCTTGGGTCCCATTAGATCCCAGTTACCTGTTACCATATCATATCCATCATGG ACGAGTACCTTGCACTTTTCCACCTAAACCACTGCGCCCTGCAGCACAAGCAAAGGTTGGTGGGACAAGA ATGCCTACCCGTAACCACAGGAATCCTGTCTCCATGGAAACCAAAAGCAGTTGCCTGCCTGTTCAGCAAG TTGAAAATGAAGGAGTGGCTCGGAATAAAAGGAAAATAATGTAAGGGCTGGACCATAGAAAATGAAGTTT CAGAAAACCCAGTTATAACAGTGTTTAATGTTGAACTTTAAGGGGAAACATACCCAGAAGATCAGCTAGT GAGAAGAGTGTCTCCCCTCGCTATCCTGGAGTTCTTACAAAGTGCCTTGGAAACCTACTGACTGCAGGAA TGGCGGTGACAGCAGGAGTTAGTCACTCCTCAGCCCTGGTGCACGGAGAATGTAGAATGCTCCATTAGCA TGGCAGCAAACTGAATAAGAAAAATATTTTTATAGCATTCTTTTCTAAAGATCATAGGTAGGAAAACCAC TTGCTGATTTAGGAACTGTCATGTATTACGTTATCAATTTGATTATATTGCCACTCAAATGAGACTGGCC CAATAGTTAAATATTGCCAATGTTAGAAACAGCTCTGTGCATTGTTTCTGACCCATTTTTGTCAGTATTA AAACTGCTGTTGATGTTTGAAAACTTGTTGAAATTCCTTTGTGGACGCCGAAAGTGAAAACAAGAAAAAT CTTCATCTCTGAAAAGTGGCAAATTAATATTTTCTTACTAGGCAACCACCCTTGTGTTTTATAAAAACAA AAAGCATTCTGTGGTGTGACAGCTTTGGTGACAATATCTCTACTACTTTGCACAAATTCCATGTAAATGT GGTAAGATACTACCCAAAGCAGAAATAACCATGAGGATGCAGGAAAAACAAAAAAAACAAAAAAAAACAC TTGATGATCCTATCAGACATGGTGAAGGTGCCATTAAGCCTAGGATTGTTTCTTTCTTCTCATGTATTGT GGTTAATTCAGAAAGCAGCATCAGCAGTGGGTTTGGCCAGTTTGCATTTGAGGGGACAGAATATGTTTAC TTTTTGTTACCTGTTTTTTAATAAACACTAAAAGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30639735 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30639735 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14638 same embryo
	EMAGE:14641 same embryo
	EMAGE:14637 same embryo
	EMAGE:14639 same embryo
	EMAGE:14636 same embryo
	EurExpress:euxassay_005568 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source