EMAGE mouse gene expression spatial database

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EMAGE:14641

Plagl2 pleiomorphic adenoma gene-like 2 ( MGI:1933165)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005549. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005549_01	euxassay_005549_02	euxassay_005549_03	euxassay_005549_04

euxassay_005549_05	euxassay_005549_06	euxassay_005549_07	euxassay_005549_08	euxassay_005549_09

euxassay_005549_10	euxassay_005549_11	euxassay_005549_12	euxassay_005549_13	euxassay_005549_14

euxassay_005549_15	euxassay_005549_16	euxassay_005549_17	euxassay_005549_18	euxassay_005549_19

euxassay_005549_20	euxassay_005549_21	euxassay_005549_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14641_wholemount_strong.wlz

14641_wholemount_moderate.wlz

14641_wholemount_weak.wlz

14641_wholemount_possible.wlz

14641_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14641_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8759
Entity Detected:	Plagl2, pleiomorphic adenoma gene-like 2 ( MGI:1933165)
Sequence:	sense strand is shown >T8759 CCGACCGCCGCCACTACCGGGGCCTGAGCGGGCCTGGCGGAGCCCGAGCGCGGCCCGGCCCGCAGCGCGG GGTCCCGAGCGCAGTGAAGAGACTGTTTTCTGAAGGCTGCGTTGGAGGCTGTGACCGAGCAGAGAGCCCA TGTGAACTGATGGGGAGGCTTTCTGAATAACATGGCCTTTTGCCATTAGCCTTGCCATGACCACATTTTT CACCAGCGTCCCTCCCTGGATTCAAGATGCAAAGCAGGAGGAGGAAGTGGGCTGGAAACTAGTTCCCAGG CCTCGGGGCCGGGAGGCAGAGAGTCAAGTGAAGTGCCAATGTGAAATCTCGGGGACACCATTTTCAAATG GGGAGAAGCTAAGGCCTCACAGCCTTCCCCATCCGGAGCAGAGACCATATAGCTGTCCTCAGCTGCACTG CGGCAAGGCTTTTGCCTCCAAGTACAAGCTGTATAGGCACATGGCCACCCACTCGGCCCAGAAACCCCAC CAGTGCATGTACTGTGACAAGATGTTTCACCGAAAGGACCATCTGCGGAACCACTTGCAGACCCATGACC CCAACAAAGAGGCCCTCCACTGCTCTGAGTGTGGTAAGAATTATAATACAAAGCTGGGATACCGTCGTCA CCTGGCCATGCATGCTGCCAGCAGTGGTGATCTCAGCTGCAAGGTGTGCCTGCAGACCTTTGAGAGTACC CAGGCCCTATTGGAGCACCTGAAGGCTCACTCACGCCGGGTAGCAGGAGGTGCTAAGGAGAAGAAGCACC CCTGTGACCATTGTGACAGGCGGTTCTATACTCGGAAGGATGTCCGACGGCACCTAGTGGTGCACACAGG TCGTAAGGACTTCCTGTGTCAGTACTGTGCCCAGAGATTTGGCCGCAAGGATCACCTGACACGCCATGTC AAGAAGAGCCACTCACAGGAACTGCTCAAGATCAAGACAGAGCCAGTGGATATGTTAGGCTTGCTCAGCT GTAGCTCTACAGTCAGTGTAAAGGAAGAGCTGAGCCCTGTGCTTTGCATGGCCTCTCGGGACGTGATGGG GGCTAAGGCCTTTCCTGGCATGTTGCCCATGGGTATGTATGGTGCCCACATCCCTACCATGCCCAGTGCG GGCATGCCACACTCCCTGGTGCACAACACACTGCCCATGGGTATGAGTTACCCTCTGGAATCTTCTCCTA TCTCCTCCTCAGCTCAGCTCCCTCCAAAATACCAGCTTGGATCTACCTCATACTTGCCCGACAAACTGCC CAAAGTGGAGGTGGATAGTTTTCTGGCAGAGCTTCCTGGAAGCCTGTCTCTCTCCTCGGCTGAACCTCAG CCCGCCTCACCTCAGCCGGCGGCAGCTGCAGCCCTCCTGGATGAAGCACTGCTCGCCAAGAGTCCCGCCA ACCTCTCCGAGGCCCTCTGCGCTGCTAATGTGGACTTTTCCCACTTACTGGGCTTTCTTCCGCTCAACCT ACCCCCATGTAACCCACCAGGGGCCACAGGAGGCTTGGTTATGGGCTACTCCCAAGCTGAGGCACAGCCT TTGCTCACCACTTTGCAGGCTCAGCCTCAAGATTCCGCTGGAGCTGGGGGACCCCTGAACTTTGGGCCTC TGCACTCCTTACCTCCTGTCTTCACCTCTGGCCTGAGTACCACCACCCTGCCTCGTTTCCACCAAGCATT CCAGTAGCCCCCATGAAGGTCCCCCAACTCAGTTTTTTGCTTCTGCCAGGGAGCCTAAGTACCCACCCCG TCCACCTCCCCCATGAAGGCAGCTGAGCTTTCAGAGCTGGGTTCAAAAAGAGAATTCTAGTGTCTATATG GAGCACTTGGTTTGGGGGTTCAGGCTCCAGGATCAATTTAGAGGGATCCTTGAACCCAGCCCCGTGAACA ATCTATCATAGGACTTTCAGGTATTTTTACTTTTATTTTTTGATCTGAACTGGGAGCTATATTTAGCCCT CTGCCTCTCCAAAGCATCAGAACCACCTTCAGTCTGGAGAAGGGGAAGCCTCTTGGAGACAGAGGGTTTC ACCTTATATGACCTCAAGAGAAACAGCCTAAGAAGCCCGCCTTCTGGTCCCAATCTGCAGACCCTCCAGC AGTTGGCTGTTGAGTCCCTGCTGTGGAAGTTCTCTTGACTATCACCTGCTTCCAGCTACCAGGCAAGAAA GAGGGCCTCTGTTCTCAACCATCCCACCCCACCCCACCCACAGCTCCCCTTCAGTCAGTGTTGTCCAGCA ACGGTACCGTTTACACATTCACCTCCGACACGCCATTTCACCTCCCTCGCCACACTGTTAGCCTTAGTGA TTGCAGTGAACACTGTTTACACACCGTGAATCCATTCCCACCAGTCCATTTCAGTTGCCACCAGCCTGAA CCACTCGGTACCTGGTGTTAACTGGAGCCCTGTTTACAAGGCGGAGTCGGGTCTTGCTGACTTCTCTTCA CTTGAGGTCACATTTTTCCCCCGTGGGGAAATAAACTGACTTTGGACTGCTTCAGTTTCTGCCATCTTCC ATGACTGCTTCTATTCCTGCCTTCCATGGCAGTGTCCACAGGAGATAGGCAAGAAGATTGGAGCAGCTTT CTCACAGGTCTGCAATTTGGATTTTCTCCAAGTACATCATGACACCTTCGTCCTCCTGAACTGGAGAGGG GAAGAAATGAAAGGCATCCCATATCAACCATTTCCTCAGTCCCTGACCCCACCCTGGACATCATGAGCAG TTATCCGTCTTGGCAGGAGGGGTTCTGGGGTTCGGGCTGGATAGCAGAGGAGAGGGATGAGCCCTGCGTT TATTTCCCTTCTGCTCGTCCTTTTCCAGGACACAATGGAACTGTCGGAAGAAGAGTTTCTAGAAAGTTAG GATTGGGCAGATAGTTGAGTCCTAATCTATCAAATCATTGTTGCCTCTCATACCAAAATGTTCTTACCCA CTGTCCTTCCCCCTTCTCTGCTTTGCAGTCCCCATGGACTCTGGGCATGGTGCACTGTCGTCTTTGCTCT TCATTGAGGATCTTCCACCCACCATGGTCCCCTCAGTTGCACCTAGTGTCTAGCAATGTGTCTTGCAAGA AATTCCTAATTCTTCTGCTGACTTGGAGCTCGCTGCCACAGACAGCACAGCCCTTGCCCAGAAGCAGCTG AACAATTCCTAACCCATTTCATCTCGGTTCTGTATGGGTTTTTCCCGTGCATGGAAGGGAGGCAGCTCAG GGACCACCATTGTGGAGCCTTTGAGACCTCTCTGATTCAGAAACACACTTCTACCTCTTTACTGTTAACT CCAACAGATGTAGCCAGCAGCCTGGGCACTTGTGTTAGTGGTGCCGTTTGCTGGCTTCTCCACTAGGGTT TCAGGACAGACTACCTAGGAACTGCCTCAACCTCCCACCAGTTCTTCCCTGCCCCTCCCTTCTGTCTAGA TTCACTTCAGTTTATTGTTGGGAACTGTGCAGGTCCAAGACCTGGACTCCTGCTTCAGCCCAGGCCAGTA CTCCTTGTCTGAGGTGAAAGCCTTCAGTTTAGCTCCCTGGCCCTTGTGTGGAAGCAATGTGGAGTGAATG AGCATATTTTGATTTAAGCAAGGTAGCTTGGAATACCTTTTTTAAAAACAACTGGATATAAATATGTCTG ACAGGATTGGAATCAGACTATGTGATTTGCTTGTAAAGATTAGTTTCAGTGAGTCCCAGGGACTAATTAA GGTTTATTTTTAAAAGCGTCTGCCTTGGTGCGCAACCACCTCCTGCATGCTATGCATGCTGTTGGGACTC GTATTACAGGGAATGGCATTGAGAAGCCAAGTGGGACTCACTGCTGCCATTCTCCTTGTGCTTGGTCTTC ACTAGACCCCAGCCCCCTCTACCCTTCTTACCACCTCCCTATAGCCTCAGCTTCAGAAGCCAGTAGCCTC CAGCCACAGAGTATCGTGTTGTGGGCTGTTTGAGAAGACCTGTCCTCCTGAGCTGCTCCTTCCCATGGTG CTCACAGGCCAGCAGAGCACCTCTTGTAGATCTTGTTCCACCTCTAATCTTTCCTCGTTGTCTGTTCTTA TGTATTCTTCAGTTTTCCCACTTCTCAGTGTCTTCACTCTAAGGACTTTTCCTGTCACTGTGAACTTTGC TGGTTATCTGCCTTCAATTTTTGGACCACAAACCACCCTGCAGCTTCTTAAACCTTTCCCCAACCTACCA CCCAAAAGAACCTTTTGGCCAGTTGTTAAAAGGACAGGAATGATTTTATTCTGTAGTTGGAGCCCAAACT CATTAAGGTACCCTTTATGAGGCCAAGAGAGCCAGAGGGGCATGCGCCCAGGACAGGAGCCATGGCATGT GGCACAGACCAATCAGGGACCAGAAAAGAATGATGTCCCTTTCCTTGCCATGTCCTTGAGCCTGTCAGTA ATGAACTGGCTGCAAGGGGACAGCACCTCTCAGCCTGCACAGCAGCTTGTCAGAGTGAGAACTGTCCTGG GGCCAGAGACTAACATGCCCAGGTGGTGTCGGCTCAGAGGATCCTAGCACCATCTTAAGCAGGTGGGAGG CAGGGAAGGGTGCACCAGCGTGTCTTTCCCTGACTTTACATGGTGTTTTGTTAATGCAAATCCGGGAAGC AGGTGTGATTGCTTTTTGCTCGTAGATATTATCCTAAGTTGAAATCCCCTAAATCTCCCTTAGTAGCCTT TAAGTTCCCCCTTCCCTTTTTGGTAGCTGTTTTCTCTACCCCTCCTCCATAATTAAAAAAACAAAAAACA GAAAAACAAACAAAAAAAGTTGTATTATACAGGATCAATATTTTGCTTTTTAACAAGGATATTTATGTAA TAAGAAACTTTGCCTTAGGTAGGTGTTGGCCAGAAAAGTCCAGATTTCTCTGTGACCTCACCCTGGCCTC TCCTGGAGCTCTGAACCTGCTGTGGAAGGAAGTGGCTGTGACTTTAACCACTGGAGAGCGGAGTCTGTGG TGTGGAGAGGGTGTTCACTGTGTAATCTTGGGCTGTGTGGAGTTGTTCTTATCACCTTGCTTCAGATGTT ACTTTGGCGTCCTCCACCAGTTCTCCATGCGCACCTCTGCATGTCCGGCACTAGAATCCCTGAACACTAC TTCACACCTCCCCCACTGCCAGTTGGTTGATGCTCTAGAGCCACTCTTTCTTAATGCCAGATTATTTAAA GAAAAATACAAGACAAAAACCTTCTAGCACTTTGTAAACACAGTGAATAACCTCTTGGAGTATTTTTGGC TTTATATAAAACAAGGTTTTTAATTGTAAAATATAAGTGCCATTAGAAAATGCACAGGGCATATCTTTGT TAAAGTAGATTTTTTCAATGTTTTACAGAATTAATTTTCAAAAAAAAGTTTTTATAATGAAGTTGTTTAT TAAAAACTTCTGAATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405960 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405960 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14638 same embryo
	EMAGE:14637 same embryo
	EMAGE:14640 same embryo
	EMAGE:14639 same embryo
	EMAGE:14636 same embryo
	EurExpress:euxassay_005549 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source