EMAGE mouse gene expression spatial database

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EMAGE:14655

Ncald neurocalcin delta ( MGI:1196326)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005524. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005524_01	euxassay_005524_02	euxassay_005524_03	euxassay_005524_04

euxassay_005524_05	euxassay_005524_06	euxassay_005524_07	euxassay_005524_08	euxassay_005524_09

euxassay_005524_10	euxassay_005524_11	euxassay_005524_12	euxassay_005524_13	euxassay_005524_14

euxassay_005524_15	euxassay_005524_16	euxassay_005524_17	euxassay_005524_18	euxassay_005524_19

euxassay_005524_20	euxassay_005524_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14655_wholemount_strong.wlz

14655_wholemount_moderate.wlz

14655_wholemount_weak.wlz

14655_wholemount_possible.wlz

14655_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14655_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

vibrissa

strong

regional

strong expression: see section 09 10 11 12 13 moderate expression: see section 21

hindbrain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15

midbrain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 15 16 17 18

vagus x ganglion

strong

regional

strong expression: see section 03 04 12 13 14

spinal cord

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

cervico-thoracic ganglion

strong

regional

strong expression: see section 05 06 10 11

cervical ganglion

strong

regional

strong expression: see section 05 06

thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5398
Entity Detected:	Ncald, neurocalcin delta ( MGI:1196326)
Sequence:	sense strand is shown >T5398 GCTGGACCTTGCTGCTGCTGCTGCCGCCGCCGCCGCTGTTGCTGTCCCTACAGCTGTGGTTGTTGCTTCC CAGGGGCTGCAGGAGCATGGACTACTAAACCCCCACTTCTTCAGCGTGAGTTGAATTCTTGTTGCCAGAA TGGGGAAACAGAACAGCAAGCTGCGCCCTGAAGTCATGCAGGACTTACTGGAAAGCACAGACTTCACAGA GCACGAGATCCAGGAGTGGTACAAAGGCTTCTTGAGAGACTGCCCCAGCGGACATTTATCAATGGAAGAG TTTAAGAAAATATATGGGAACTTTTTCCCTTACGGGGATGCTTCCAAATTTGCAGAGCATGTCTTCCGCA CCTTCGATGCAAACGGTGATGGGACAATAGACTTTAGAGAGTTCATCATAGCCCTGAGTGTGACATCAAG GGGCAAGCTGGAGCAGAAGCTGAAGTGGGCCTTCAGCATGTATGACCTGGATGGCAATGGCTACATCAGC AAGGCAGAAATGCTGGAGATTGTACAGGCGATCTACAAAATGGTTTCCTCTGTCATGAAAATGCCTGAAG ATGAATCAACCCCAGAGAAAAGGACAGAAAAGATCTTCCGCCAGATGGATACCAATAGAGATGGAAAGCT CTCCCTGGAAGAATTCATCCGAGGGGCCAAAAGCGACCCATCCATTGTGCGTCTCCTGCAGTGTGACCCC AGCAGTGCCGGCCAGTTCTGAGCCCTGCACCCCCAGGACGCTACAGCTGCTTGTGTCCCTCCTGATTAGC TTTTAAACTTACTTCGGTTTTTGTTTTTGCCAAACAATATTGATGGTGACACTGTGCCCTCTTCAGTCAG ATGTGCCCTCCTGTGACACTTTTGCCCCTTGCTTCTTTCATGGTGGATGTGAATGCCCAGAGCCCACGTG CTTGTGGAGAGCATGCACAGACTTCGTGGTGTTCCCCAGTGGATGGCTTTTTTAATCATCTTTTTTTTCC CCTTTGATTCTAATGTCTCTATCTTAAAACCTAAGCCCTGAGGGGCAAGACAAGGGAAGTACATTCAGCC CGGGGCTTCTTCTGCAAGCTTCCAGGGGCTTTGTTTGAAATACAAAACTCCTACCCTGGGTCTGTTGTCA CACATGCCCTGGGAGCTAGAGCAGACACCTGAGGCTAAGTTTTTAAAGAACAGGTTACCCTCTGGGGAGA GGCTATTAAAGGCAGCTGGGACATTTCCCCCCAGAGGCCACTCCCTCTCACCCCAGCAGGCTGTAATTTC TAAGCTGTGAACTTTTCAAGGTAAATTAACAGAGATGGGAAAAGATGGCTCAGCTGTTTGTTTTGTTTTG TTTTGTTTTGTTTTTCTCCCACAGGTTTACATAGTTGAGCTAATACAAGTTTCTTTAAAAAAAAAAAAAA TCAAACACATATTCAAATGCCAGACCCATCTTGCTGCCTACTGGTGATTTATAAAAGACTGCTGTATATA AAACATTGGATATTGCAGACCAAATTAAGTGTTTTGCCTTGTAAAATGCATGTTTAGCGAGCGCTAATAC AATCTTACTACAGAAGACTGTTTTAGTAGCTTATTGTGAAGCCAACTACAATGAATGTCGATGTCTTGTT TTAAAGTCATAGCTGTCTTTGCACAAATGTACCAGTTAGCAAGTATATTTTATATACTCCATAAAATCAC AGAATGGTGACAGGGGCCCAGCTTTGATTTCAAATGGCTTCCTAGAGTCAAGGTGGCTGGAGGACACGAG GAAGCCAACTGTCCAGGCATCCTGTCCACCAGAGCAGCACGGTGAGCTCCACAAGGACAAGGGACCAAGG CCCAGCAGCGTACTGGCAGCAAGCGCAGGTGCAACGCTTGTCTCAGCAGATGAATGTCCTACAGACGCAG AAAAACAGAGACTTCAAGCAGTTTAACCCACATCCTTGGTGGGGGAGCCACATCTAGAATGTTCATTTTT CCTGCCCCCAGGTACCCTAGTCAGGTGTGGGCTAGCCAGGTGATTCACCCATTATAAGAACCAGCAAAGG GACCTGCAGTGTCCAAGGCCAACCATGGGGGTCCCAAGAGCGCTCTTCCTCACTGTGTTCTTTGAATGCA GCAAAGTTGAGCTGCTGCCTCGGCTGGTCACCTCATGCACACTACTTTGAAATTAGGGCTGGAAATAAAA ACTCCTGGCCACAGGCAAGATAAGGCCTCACAAACAGCTTACATCAGAAGATCTAAATACTCAAGAATGT TGGTGGCAGACCCTAAGAGGAGCAGCCTAAGAGGAGGCAGGAAGGTCTCTGTCTGGCAGGGGCCAAGCCG TGTCCTGTAAAACAAGAACCACAAAAAGATAAAGACAACCTGAAGGAAAGTTTCCTGCCAGCCCCAGGTC CCCCACAGTGACACTCGTAACTGGCAGAAGTTTAAACTTGAATGGTGAATTAAAATGAATCACTTAGTAG GTTGAAATTTAGAGACCTAGATTTTGTAATCTTAATCTTTCCTAGTGTCGTTTCCTTACATTTTGTACCA AGGTTTGCCAACTCCTGTGGACCAAATGGTAAAGTCTGTTTTTGTAAAGAAGAATTAACTCAAGTGGTTG AGCATAAACAGGTGAAGGGAGCCGACATGCTGGTTTGTGAGGTAGGCTCACCTACTTGGGCTCTGCCTCA GGTTGGGCCAAACCCTGTGTTTCCTAGAACTTAGAGATGGGGTGGGAACTAGAGGTGCTCTCCAGCTATT AGGAAATGAAAGAGCCAGATGCAAGAGTTATGAACCCTGTGGTCCCCAGAAGGCATGGAGAGTTCCAGAA CTGGTTACACCACAGACCTAACAGATATATCCGTACATCCTCCTCCTCCCCACCTGGCCCCAGGAAGGCA CTGGACCTGTGTAATTTTCATGCATGCATGTTGGACCAAGGGACTTCACAGCATCAAGAGGGCCGAGTGC CTGAAGTCAACTGTCACTCCTGTGGCTAGGCATTGTTTTGCTGTGTTCAGAGATCTCTGTGGAGGAAATG GAGCCTGTTTTGTTCCAAGTAAAGGCAGCTTGTGCTGTCCATGGAACTGGAGTAAATGGCTTATGAGTTG AAGGATGTTTCAGAATGCTGGACTGTTCATACCTCGCTTAGGATGGAGGAGGGGATGCTGTGGGTACCAC CCCACCCCAGCAGTACTCCCTGGGGACTTTCTACAGATGAATTTAATAATATATATGTCATACTATGTGG AATGGTCCCGTTATCAGTTGGTTGTGTAGCTCTTGCCTGGCATTAAAGCATGTTTATTATTTAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365560 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365560 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14656 same embryo
	EMAGE:14658 same embryo
	EMAGE:14657 same embryo
	EMAGE:14654 same embryo
	EMAGE:14659 same embryo
	EurExpress:euxassay_005524 same experiment
	MGI:4826608 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source