EMAGE mouse gene expression spatial database

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EMAGE:14657

Zfp248 zinc finger protein 248 ( MGI:1919970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005531. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005531_01	euxassay_005531_02	euxassay_005531_03	euxassay_005531_04

euxassay_005531_05	euxassay_005531_06	euxassay_005531_07	euxassay_005531_08	euxassay_005531_09

euxassay_005531_10	euxassay_005531_11	euxassay_005531_12	euxassay_005531_13	euxassay_005531_14

euxassay_005531_15	euxassay_005531_16	euxassay_005531_17	euxassay_005531_18	euxassay_005531_19

euxassay_005531_20	euxassay_005531_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14657_wholemount_strong.wlz

14657_wholemount_moderate.wlz

14657_wholemount_weak.wlz

14657_wholemount_possible.wlz

14657_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14657_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9601
Entity Detected:	Zfp248, zinc finger protein 248 ( MGI:1919970)
Sequence:	sense strand is shown >T9601 CTGCAGTCCTGCTCCTGGTCCATCCGTGGCCTCGCGTACCTCCTGGGAAACAACTCCTGGCCTTCGGCAC GGTGCACTGCAGGAGCGGCGGCTACTACTAGGGAGCCCACCCTCGCAGGGGGCCTTTGGACTGGTCCCTA GGGCGCAGTGTTTGGAGTGGAAGAGGAAAGTGGTTAGCTAGCCACCAAACCTCCTGCCCCCTCACACACC CGGGATCCCAGTTCTGAATTTGGTTCTCGCTCATGTGTTTTGTGAAAATGACAGTGTCCTGAAACCATGG AAGACTGTGTGCTCGCCGAAAGTCTGGGACAAGTTTAAGGAGAGAGCTTTCCCTCCTCCACTCAGAGCAG AAAATGAACAAATCCCAGGAGCAAGTGTCGTTCAAGGATGTATGCGTGGATTTCACCAAGGAAGAGTGGT ATTTGCTGGACCCTGCCCAGAAGGTGCTTTACAGAGATGTGATCCTGGAGAATTATAGCAACTTCGTCTC CGTAGGGCATTGTGTTACTAAGCCAGATGTAATCTTCAAGATAGAGCAAGGAGAAGATCCCTGGATACCA GAAAATGGATTCCAGTGCCACCCAGAAAGGAAATGGAAAGTGGACGACCTATTAGAGAGCAACCAGGAAA ATCAAGATGGACATTTTTGGCCACTCATGTTCACCAACAAGATCACAAACCTAGAGGGTGGAGGTAACAC AAGAGAAACTCCCAGTCCAGACATTGACTCTGTATCTTCAAGAAGTTTTCTGCTCAAAGTATGTGACTCA TGTGAAATGAATTTAAAGAATGTTTCAGCTGTAATTATTAGCAAAAAGAACTATTCCAGAAAGAAGCTTA ACGAGTTTAATATACATAAGAAACCACTCCCTGACAGTAGGCGTGAGAAGATTCCTGTAGGAATAAAGTC ATGTGACTATGATGGGGAAAGGGATGGCGTCCGTCTGTGCCGGGATCTCGCTCGGCCAGTTTCTCATCAG CCTTTTGAGTATAACGAAACAGGACAAGGCTTCTGCGAGGAAGTGGCCCTTTGTACAAGTCAGAACTCTC AGCTAGGGGAGACTCTCTATAAGCACACTGAATGTGGAAGAACTTTCGTTGACAGTTTGAAGCTTAGTGT ATCTCAGAAAACACGTTTGCATACAGAACTTCATGAGTGCAGTATTTGTAGAAAGTCCTTCTATGTGGAT TTGCAGTTTGGACATCACACAGCTCTCTCAGGAAGCAGTCCTTACGAGCGTAACGAATATGAGAAACTCT TCTGTGACGGTACAGCTTTCATCGTCCATCAGGAAGCTTACACGAGAAAGATCGCCCAAGAGTATAAAGT GAGTAACAGAACTTGGGGAAAGCCAGCTCTCTTCAAACATCAGATAATACAGATGGGAGGGAAACCCTAT GAATACTATGAAAACAGGAAAAATTTCAGCAAAAAATCACATCCCACCCAGCCTCGAAGACCTCACGTGG GAGAAAAGACGTTTGAATGTGGTGAATGTGGTAAAATGTTTTGGGAGAAATCGAACCTTACCCAGCATCA GAGAACACACACCGGAGAGAAACCGTATGAGTGTACCGAATGTGGGAAAGCCTTCTGCCAGAAGCCACAC CTTACCAACCATCAGCGGACACACACGGGAGAGAAACCCTATGAGTGCAAGCAATGCGGAAAGACGTTCT GTGTCAAGTCAAACCTTACTGAGCATCAAAGGACACACACTGGGGAGAAACCCTATGAATGCAACGCATG TAGGAAGTCCTTCTGCCACCGGTCAGCCCTAACTGTCCATCAGAGAACACATACAGGAGAGAAACCATTC ATATGCAGTGAGTGCGGGAAGTCGTTTTGTGTGAAGTCAAACCTGATTGTCCATCAGCGAACTCATACAG GGGAGAAACCCTACAAGTGCAACGAGTGTGGGAAAACGTTCTGTGAGAAATCGGCGCTCACCAAACACCA GAGAACTCACACAGGGGAGAAACCCTATGAATGTCACGTGTGTGGGAAGACCTTCAGTCAGAGGTCGGTA CTTACCAAGCATCTGAGAATCCACACGAGGGTGAAAGCTCTTTTAGCATCCTGAGTGTTTAGCTTTCGCA TGCCTGTCAAGTCCATTGTAGTTTTAAAAATGAGCTTAGAGCCTGCCAAAGAATACCACAGAGTACTAGA AAATACCATCGTGTTGGAATGCATGGAAGCTTTCAAGAAAAGAAGAAACTTTCATTTGAAAGATTGTATT GAAGGGAAATTATTCATATAAATAATATGGTAATAAACTTAGAAATTAAATTGTATAATGTCTTCCAAAT CTGTCTTTTAAATATAATGGTTAATATTAATTTTACTTGTATTCATAGGAAAGTATGAAACTTAAAAATG AGCGTCTACAGCATTGACCATACATGTAAAGACAGCCGGTGTTTATAGACCTTGTGTGAGTATGTTAATG TAACAGAAGTCTAGGAAACCCTGGATTTATGATTTATTGGAACCCAGAAAGGATGTGAGGAAGATGAGCC CTTAGTTTAGCAACTGTTATGGAATACTTTCACACTAAATACCACCAGCGTTTTTATAGGTTGATAGGAT TATGTACGCTTCTGAACATATTTGTGAATGTGATCTAGATGTGTGAATATGTAAATATACCTGTATGCAT GTACCTGTACACAAATGGTGTTGTCACAAAGGCAGCTCACACCATTTAAAAGTGCTGATGATTGTTAAAA TGTCAGGATTTTTGTGGCTCAATTGTCTAATATTACATGAAATTGCAGCTCAATTATACTGAGAGCAAAG GAAATAAACAACACTTGTCATTCCTAACACTCTGCTGTATCTCACTGCTGTCTTGGCTGTGTACCATCGG TCTCCATGCTGAGAGTGCTGTAGAGTGGTTCACTATTGTATATCTCCTTCAGTTACCTCCCATGTGTGCG TGCACACACACACACACACACACACACACACACACACACACACACACACACACGCCATTGCTCCGCTCAT GTTGGTAGCTTGCCCTTAGGACGTTTATACTGTAGGAACTGATAAAGCATGTGTCAGAGCTTGATTTTCT CAGAGAAGCCGTGTGTATATAAACTATGTGGTAGATTTAAAATATAAACTGCCCCTCTTTGTATTTCCTA AAATAAATAAATGGCTCTGGCCATCATTGTCAAATTGCCTCTATAGTAAAGAAGCCAAAGATGGTTTACT GAGCCTTTTAATTACCTCAGAACTATCCAGTTCTAAATACTACCAGTCTCGATAGCTTGGTCAGTATATG CTTCAAGTAACATTATTATATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT CTCTCTCTCTCCCCCCCCCCCCTTCCCCTCTCTCTCACACACACATATATTATCACAGACTTTTCACTAT TTTGGACCATGAATAAGTATGGCCATTATTACCAAACTACCCATTTGGTGAGGAAATGAAACAGTATATT TCTGAGAGTTTTTCTTAGCTACCTCTATGTCAATCATATAGATGCTGTTAATAAAAGTGATATTTACATG ACTTAACTCGAGTTGTCAATGGTAATGCTACACTCTACAAATTTAAAGATGGTAGTATATAATAAAATGT TCATATTATACCCTTGGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30615600 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30615600 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14656 same embryo
	EMAGE:14658 same embryo
	EMAGE:14655 same embryo
	EMAGE:14654 same embryo
	EMAGE:14659 same embryo
	EurExpress:euxassay_005531 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source