EMAGE mouse gene expression spatial database

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EMAGE:14662

Rbm41 RNA binding motif protein 41 ( MGI:2444923)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005537_01	euxassay_005537_02	euxassay_005537_03	euxassay_005537_04

euxassay_005537_05	euxassay_005537_06	euxassay_005537_07	euxassay_005537_08	euxassay_005537_09

euxassay_005537_10	euxassay_005537_11	euxassay_005537_12	euxassay_005537_13	euxassay_005537_14

euxassay_005537_15	euxassay_005537_16	euxassay_005537_17	euxassay_005537_18	euxassay_005537_19

euxassay_005537_20	euxassay_005537_21	euxassay_005537_22	euxassay_005537_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14662_wholemount_strong.wlz

14662_wholemount_moderate.wlz

14662_wholemount_weak.wlz

14662_wholemount_possible.wlz

14662_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14662_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5423
Entity Detected:	Rbm41, RNA binding motif protein 41 ( MGI:2444923)
Sequence:	sense strand is shown >T5423 CCACGCGTCCGCTCCTTCCTATTTACCCAAGTTAGTGATAGGGGCCTGCGGAAACATGAAGAGGGTAAAT AGCTGTGTGAAGGATGAAGAACATGTCCTGGAGGAACTGGAAACAGAAGGTGAGAGGCAGCTCAAGAGCC TCCTTCAACATCAACTCGATACCTCTGTCTCCATTGAAGAATGTGTCTCTAAGAAAAAGAGCTTTGCGCC TGGGACTATGTACAAGCCCTTTGGGAAGGAAGCAGCTGGGACAATGACTTTGTCCCAGTTCCAGACACTA CATGAAAAAGACCAGGAAACTGCTTCTCTCAGGGAATTAGGGCTCAATGAAACTGAAATATTGATCTGGA AAAGCCATGTTTCCGGTGAAAAGAGGACAAAGCTGAGGGCAACTCCCGAAGCAATACAGAAACGGCTTGA AGATATTAAAGAAAGAATCTCAGAGCGCCAGCGCATCCTTTGCCTGCCACAGAGGTTTTCAAAGAGCAAA CAGCTGACCCGAAGAGAAATGGAAATTGAGAAGTCTTTATTTCAGGGAACTGATCGTCACTCCTTCCTTA AGGCTCTTTATTACCAAGATGAACCCCCAAAGAAGAACAAAGGTGACCCCATGAACAACCTGGAACATTT TTACCGCGAGACTATAATGAAAAAGCGTCTTGAAGAGTTCCAACTTTTGAGAGGTGAATCCTTTGCTTGC CACTCACTGGTCTCAGCTGCATCCGTGAGTGGTAGTGGCACAGCTGAGAAGCCATCACTCCTCCAGGACA AGGGAAAACAGGCAGCACAGGGTAAAGGCCCCAGGCTCCATGTGGCAAAGCTTATTGATTTTCCAACTGA GCAATATTGGACTGGGCCTAAGACGCTGAAGCAACCAATAGAATTTATCCCAGAAGATGAGATCCAGAGA AATCGTTTGTCAGAGGAGGAGATCCGAAACATTCCTATGTTTTCTTCATATAATCCAGGGGAACCAAACA AGGTGCTATATCTGAAGAACCTTAGTCCTCGGGTGAAAGAAAGAGACCTTATCTCATTATTCGCTCGATT CCAGGAGAAGAAAGGACCACCCATTCAGTTCCGAATGATGACTGGACGCATGAGGGGGCAAGCTTTCCTC ACCTTTCCCAATAAAGACATAGCATGGCAAGCACTGCACCAAATTAATGGATATAAACTCTATGGGAAAA TATTGGTGATAGAGTTTGCAAAGAGCAAAAAGCAACAGTCAGCTGTTCAGCGCTCTTCCCTTATAACATC TGCTCCTGATGGCAGAACAGAAATCAATGGCAGCTAGGAATTCGTATCTACACACATGTACATGCACATT TGGATATATGTATGTAAATGAATATTCAGATGATTTTTCTTTTCTCATTTCTTAGATCAAGAATATGTAA ATTCATAAAAATTTGTTGAAAATTGAAGAGGAAATATAAGAAAGAAATTAGGACAGTTGCTCTCAGCATT TGGAGAGAATTGATAAAAGTATTTTCTTTTTTTAAGATATATTTATTTATTTTATGTATGTGAGTACAGT GTAGCTGTCTTCAGACACACCAGAAGAGGGCATTTGATCCCAGTATAGATGGTTGTGAACCACCATGTGA TTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGTCAGTGCTCTTAACCTCTGAGTCATCTCTCCAC CCTAAAAAGTATTTTCTATAATTAAATACTGTATAGTATTGAAAAAGATTGTATAAAATTGACAATATCC TGGGTGTTAAAGATAATAATGAAACACTATATATGGCAGAAAATACCCTGAGGGGTAAGACCATGTGAAA ACCTTTAATTCTCAGGATACATTACATAGGACTGTTCCTAAAGATCCCAAAAATCCTTTAATAATTTTGT AGTCTTCAGTGTTTAGTCCTAGAGATAAGACTTTTTCTAGGAGTGTTTCCTGTATACGAAGTTTTAAAAC TAACCCAGTGTTAAAATATTTCTTTACTGAACTCAAACTGTGTTTTGTCAGAACCTTCAACAGTTTCAGT AGGTAGATTCATAAGTTTATTAACTTAATGATTGCTTCCACACTAAGTTTTCAGAGTCCTCAAAAGCATA TTTATTATTATGACATCTTTTAAACCAAAATATTGGTAGTCTATGATACTATTGCATTCAAAACAATATC CATTAAACATGGAGTATCTGTCTAATGTATGAACTTTCAAGACAAATGAACTAGCATCTATATTAAAGGG ACAGGAAAGAAAGTTCTTTAGAGGAAAGGCAGCCTGCCTCAAATTCCCTAGTGATTCGTTTATAGTTGTT GAAGAAGAAGAAGAACCTAAATGGTTTAGACGGTAGAACCAGCTTTGGAGTCAGACCTGTGACCCTCAAA GTCACCCATGTTTACCATCCTGAGCTTAGAAAGTCACTCACTCTGAGCCTCATTTATCTCATCTATAAAA AGTAACATTGCTTCTGACCTCCCTGAGTAAGGGTTAAATGAGATTATAATATCCAAGGTATTATATTATA TTATATTATATTATAACAAGGGGAAAAATGAACTTTAGGGTACTCCACTAAGTACATCAAACAACATATA ATAAGAATTTCATTCAAAAGCGAGAAAGAAGCATAAAATACTACAAAAGGTCGAGTGAAATGGCTCAGTG AGTAAAGGCATTTGCCACCAAACAGGAAGACCTGAGTTCAAACCCTCAGACCTGCCAATAATAGGACAAA AAGGCTGGGAAATAATGCTATAGTTTCTTTAAAAATCCTGTAAATCCATAGGAACAAATAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5368185 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368185 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14663 same embryo
	EMAGE:14661 same embryo
	EMAGE:14666 same embryo
	EMAGE:14665 same embryo
	EMAGE:14664 same embryo
	EurExpress:euxassay_005537 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source