EMAGE mouse gene expression spatial database

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EMAGE:14663

Zfp759 zinc finger protein 759 ( MGI:2446280)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005518. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005518_01	euxassay_005518_02	euxassay_005518_03	euxassay_005518_04

euxassay_005518_05	euxassay_005518_06	euxassay_005518_07	euxassay_005518_08	euxassay_005518_09

euxassay_005518_10	euxassay_005518_11	euxassay_005518_12	euxassay_005518_13	euxassay_005518_14

euxassay_005518_15	euxassay_005518_16	euxassay_005518_17	euxassay_005518_18	euxassay_005518_19

euxassay_005518_20	euxassay_005518_21	euxassay_005518_22	euxassay_005518_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14663_wholemount_strong.wlz

14663_wholemount_moderate.wlz

14663_wholemount_weak.wlz

14663_wholemount_possible.wlz

14663_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14663_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5402
Entity Detected:	Zfp759, zinc finger protein 759 ( MGI:2446280)
Sequence:	sense strand is shown >T5402 CCACGCGTCCGCGCTTCTCAAATTTGTGCAGTTGTCCTGTTGAGCGCTGCATTTGGATTTCTGACATGGA GAATATGCTGTCATTCTGGGATGTGGTAATATATTTCTCTGCAGAGGAGAGGGAATACCTGGGTCCTCCT CAGTGGAAATTGTACAGGGATGTGATGCTGGAGAATTACAACAACCTTGTCTTTCTAGGTCTTGCTTCCT CTAAGCCATACCTGGTCAGATTTCTGGAGCAAATACAAGAGCCTCTAGATGTGAAGAGTCAAGTGGCCAT CTCTATGTACTCAGGGGGAAATAGCTCTACATGCAAAGAAGTTTCTGAGAGGGAAAAAGTACTTGGAAAT CAGCAGATAATTCATTTAGGTTTGAAGCCATATAGTTGTGAAGAGGTTGGCAAGTCCTTTTGTTTTTCAT CATTACTTTCTGAACACAAAAGAATCCATACAGGAGAAAAACCCTACAAGTATGAAACATGTAGCAAGGC CTTCCATGTTCCATCAAAACTTTCTCAACACAAGATAATTCGCACAGGAGAAAAACCCTACAACTGTGAA GTATGTGCCAAGGCCTTCAAATATCCATCAAGACTTTCAAAACATAAGATAATTCATAAAGGTGAGAAGC CATACAAATGTAAAGTATGTGGAAAGGCCTTCTTCTCTCCATCATCTATTTGTCAACACGAGAGAATTCA TACAGGAGAGTTACCCTGCAAGTGTGATGTATGTGGCAAGGCTTTCCAATATCCATCATTACTTTCAAAA CACAAGATAATTCATACAGAACAAAAACCCTACAAGTGTGAAGTATGTGGAAATTTCCTCCGCTCTCGAT CATCACTTCATGAACACAAGAGAATGCATGCAGGAGAGAAACCCTACAAGTGTGAAGTATGTGGCAAGGC CTTTGAATATCCATCAAGACTTTCAAAACATAAGAATATTCATACAGGAGAGACACCATACAAGTGTAAA GTATGTGGAAAGGCTTTCCGCTCTCCATCATCACTTCGTGTACACAAGGGAATTCATAGAGAAGACAAAC CCTACAAGTGTGAAGTATGTGGCAAGGCCTTCCGTTATTCATCAGTACTTTCAGACCATAAGAATATTCA TACAGGAGAGACACCATACAAGTGTAAAGTGTGTGGAAAGGCCTTTCGCTCTCCATCATCACTTCGTGTA CACAAGGGAACTCACAGAGGAGGAAAACCCTACAAGTGTGAAGTATGTGGCAAGGCCTTCCATTATCCAT CAATACTTTCCAACCATAAGAATATACATACAGGAGAGACACCATACAAGTGTAAAGTATGTGGAAAGGC CTTCCACTCTCCATCATCACTTATTCTACACAAGGGAACTCATAGAGAAGACAAACCCTATAAGTGTGAA GTGTGTGGCAAGGCCTTCCGTTATCCATCAATACTTTCCAACCATAAGAAAATTCATAGAGAACAAAAAC CCTCCAAGTGTAAAGTAGGTAGAAAGGTCTTCTGGTCTCCATCATCACTTCCTGAGCAGAAAAGAATTCA TACAAGAGAGAAACCCTACAACTGTGAAATATGTGGCAAGGCCTTCAAATATCCATCAAGACTTTTAAAA CATAAGATAATTCATACAAGAGAGAAACAATACAAGTGTAAAGTGTGTGGAAATGCCTTCTGCACTCCAT CTTCACTTTGGCAACACAAGAGAATTCATACAGGAGAGTTACCCTGCAAGTGTGATGTATGTGGCAAGAC CTTCCGCTTTCCATCATTACTTTTAAAACACAAGATAAGTCATACAGACCAAAAACCCTACAAGTGTAAA ATATGTGGAAATTTCCTTCGCTCTCAATCATCACTTCATGAACACAAGAGAATGCATGCAGGGGAGAAAC CCTACAAGTGTGAAATATGTGGCAAGGCCTTTGAATATCCATCAAGACTTTCAAAACATAATAACATACA TACAGGAGAGACACCATACAAGTGTAAAGTGTGTGGAAAGGCTTTCCGCTCTCCATCATCACTTCGTGTA CACAAGGGAATTCATAGAGAAGATAAACCCTTCAAGTGTGAAGTATGTGGCAAGGCCTTCCGTTATTCAT CAGTACTTTCTGACCATAAGAATATTCATACAGGAGAGACACCATACAAGTGTAAAGTGTGTGGAAAGGC CTTCCGCACTACATCATCACTTCATATACACAAGGGAATACATAGAGAAGACAACCCCTAAAAGTGTGAA ATATGTGGCATGGCCTTACAAAATCCATCAGTACTTTCAGACCATAAGAAAATTCATACAAGAGAGAAAC CTTAAAAGTGTGAAGTATGTGGCAGACTTTCCAGTATTCACCATTACTTTCTAAACACAAGATGATTCAT ACAGAAGAGACTCCCTACAAGTGTGAAGTATGTGGCAAGGTCTTTACTTATCAAAATGCTAAACAAATTT AGGGATCATCAAAGAATTCATTCTGGAAAGAAACCATACAAATGTAAAAAATGTGGCAAATACTTTTGAA TTTCATGGCATGGTCTGAACACAGAATAATTCTTATTGGAGATAAACACTACGCAAGTGGAGAATGTGGC AAACACTGTATTACCGGTCTTTGGGTCTTAAGCAACATCAAAGAATTCATCTATGAGAGAAACCACACAG TGTGAAGAATGTGCTAAGGTCTTTTGGACTTACAGAAACCTTGGGGAAAACCTCATAATTCATACTAGAC TGAAATGTCACAAGTATGAAAAATATTGCAAACCCTTTAATGAGCATTCTTATATTTCTGAAAACAAGGT AGCTTGAACTAGAGTGAAATAGTACAAATGTTAAAATGTTCAAAAAAATCTTAACTTCTTCAAACCTAAA AATTGTCAAAAGCTTCATGCTGAACCGAAGCCCTATAAGTTTTGGGAATGTGGTAAAGGCTTTGGAATCA TTCATAAAATCATGCCAAAATTGGAGCTCTCAGACTGTGTGTTTGTCCTTCTGGATTTGGGGCTGATTCT TTCTTACTGTCTTTTTGTTTCTTCTGTTTTAGAATTGAAACCCTTGTTCTATACCAGTATGTAAAAAGAA GTCACTTGTTCTTATGTTGTAGAAGCTCACAGTCAAGAGATTTCCTTGAGTCTCATTTGACACATGGGCT GTGGTCTTTCATAGTGTTGTAATTGTTTAATCCTTTGTGGACCTTTCAAGTTGTAGGCTGTTTGACTTCT GAGGTGGGGCTAAGGTTGGGGAAAGGGAAGGAATGTTATGATTTAAAACTTTGTGGCTAGAGCTAGCATG TTGACTTTGGAAACAAACTGAATTTCCTATAACCCCCACATGCATGTTAGTATTCCCAGTACTCACAAAG TAGAAGTAATTACCTCTGAAGGTTTTCCTCTGACCTCCATGAATAGGCTCCCACACAAACATAAATTTGT GTGCCCAGAAATAAATAAACATAATTATGCTTTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365910 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365910 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14661 same embryo
	EMAGE:14666 same embryo
	EMAGE:14662 same embryo
	EMAGE:14665 same embryo
	EMAGE:14664 same embryo
	EurExpress:euxassay_005518 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source