EMAGE mouse gene expression spatial database

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EMAGE:14664

Tdrd7 tudor domain containing 7 ( MGI:2140279)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005556. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005556_01	euxassay_005556_02	euxassay_005556_03	euxassay_005556_04

euxassay_005556_05	euxassay_005556_06	euxassay_005556_07	euxassay_005556_08	euxassay_005556_09

euxassay_005556_10	euxassay_005556_11	euxassay_005556_12	euxassay_005556_13	euxassay_005556_14

euxassay_005556_15	euxassay_005556_16	euxassay_005556_17	euxassay_005556_18	euxassay_005556_19

euxassay_005556_20	euxassay_005556_21	euxassay_005556_22	euxassay_005556_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14664_wholemount_strong.wlz

14664_wholemount_moderate.wlz

14664_wholemount_weak.wlz

14664_wholemount_possible.wlz

14664_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14664_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

strong

regional

strong expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5420
Entity Detected:	Tdrd7, tudor domain containing 7 ( MGI:2140279)
Sequence:	sense strand is shown >T5420 GTCCCCGGGACGAGGCAAAGATGCTGGAGGCAGATCTGGTTTCAAAGATGCTAAGGGCTGTCCTGCAGTC TCATAAGAACGGGATCGTATTACCCCGTCTCCAAGGAGAGTACCGATCTTTGACTGGAGACTGGATCCCC TTCAAGCAGCTGGGCTACCCTACTTTGGAAGCCTACCTGAGAAGCGTGCCAGCAGTTGTCAGGATAGAGG CTAGTAGATCTGGAGAGATCGTCTGCTATGCAGTAGCCTGCACAGAAACTGCAAGGATCGCTCAGCTTGT GGCTCGTCAGAGGACCTCTAAAAGGAAAATAGGGCGACAAATTAATTGTCAGATGAGAGTGAAGAAAGCC ATGCCGTTCTTTCTAGAAGGAAAGCCAAAGGCAACTCTCAGACAACCAGGATTTGCCTCAGATTATTCCA TCAGCAGGAAACCTAATTCAGCGCTGTTAAGAGACAGAGGAAGCGCCTTGGGAGTTAAGGCTGACGTGGA CATGCCGCCGTACCCAGACACTCCAGTGCAGAGGCACGCGAGCATGTCTGCCAATAGCAGGTTCAGTCCA AAGTCATCCTTGCCAGCGTCTTTCCAGACACACATCTCAAGGGCCTGCCCTACGGAAGTCAATGATAATT TAAATCAGACTGTTGAGAAACCAAATATCACGCCTCCTGCCTCTTACACTAATAAGATGGATGAGGTTCA AAACCGCATAAAGGAAATATTAGACAAGCATAACAATGGCATTTGGATATCTAAGCTTCCACATTTTTAC AAAGAGTTTTATAAAGAAGACCTTAACCAAGGAGTTTTACAGCAGTTTGAACACTGGCCTCACATTTGCA CGGTGGAAAAACCTTGTGGTGGTGGTCAAGATTCACTTCTTTATCCTGCCAGGAGAGAGCAGCCCCTGAA AAGTGACCAGGACCCTGAGAAGGAGCTCCCACCTCCACCTCCTGCTCCCAAGCAGGAGGTTCCATCCCAA GGGAGCCCAGCAGTGATGCCAGATGTCAAGGAGAAAGTGGCAGAACTGCTGGGCAAGTACTCAAGTGGCC TTTGGGCAAGTGCGCTCCCCAAAGCATTTGAGGACATGTACAAAGTCAAGTTCCCTGAGGATGCCTTAAA AAATCTCGCCTCGCTCTCTGATGTGTGCACCATAAACTACATTTCTGGAAACACCCAGAAGGCAATTCTC TATGCGAAACTCCCATTGCCCACTGACAAGATCTTAAAGGATGAAGGGCAGGCACAAGGAGATTTTGACA TCAAGTCGATGATTGAACAAGAGTATTTGCAGATAGAAAAAAACATGGCTGAAAGTGCTGATGAGTTTCT GGAGGACATAACTGTGCCGCCTCTGGTAATTCCTACCGAGGCCTCACCGTCTGTGTTGGTGGTTGAGCTG AGCAACACAAATGATGTGGTTATCAGGTATGTAGGCAAAGACTACTCAGCTGCTCAGGAACTGATGGAGG ATGAGATGAAGGAGTTTTACAGTAAGAACCCCAGAGTCACACCGATCCAGACTGTGCACGTGGGGCAGCT GCTGGCAGTCAATGCAGAGGAGGATGCCTGGTTGCGAGCACAGATCATTTCCACGGATGAGAACAAGATA AAGGTGTGCTATGTTGACTATGGCTTTTGTGAAAACATTGAGAAGAGTAAAGCGTACAGACTGAACCCAC GGTTCTGCTCGCTTTCCTTTCAAGCCACCAAGTGCAAGCTGGCAGGGTTGGAGGTTCTGAATGATGACCC TGACCTTGTGAAGGCGGTGGAATCACTAACATGTGGGAAGATCTTTGCTGTGGAGATCCTGGACAAGTCA GATGTCCCGCTGGTGGTGCTGTACGACACGTCAGGAGAGGACGATATCAACATCAATGCCACCTGCCTCA AGGCAATATGTGACAGATCTCTACAAGTTCATCTCCAGGTGGATGCCATGTACACAAATGTCAAAGTGAC CAACATTTGCTCTGATGGAACACTCTACTGCCAGGTGCCCTGCAAGGGTCTGAATAAACTCAATGACCTT CTGCATAAGACAGAGGACTACTTCCACTGCAAGCACATGACCTCTGAGTACTTCATCTCGCTACCCTTCT GTGGGAAGATCTGCCTCTTCCATTGCAAAGGGAAATGGTTACGAGTGGAGATCACTAATGTTCATAGCAG CCGTGCACTCGACGTTCAGTTCCTGGACTCTGGCAATTCGACATCCGTGAAAGTGTCAGAACTCAGAGAA ATCCCACCGCGGTTCCTGCAGGAAATGCTTGCCATACCCCCTCAGGCTATAAAGTGCTGCTTGGCTGATC TTCCACAGTCCATTGGCATGTGGACACCAGATGCTGTTCTGTGGTTAAGAGATTCTGTTTTGAATTGCTC GGACTGTAGTATTAAGGTTACAAAAATGGATGAGACCAAGGGGGTTGCCTATGTGTACTTATTCACTCCC AATAACTTCCCGGATCCCCATCGCAGTATTAACCGGCAGATCACAAACGCAGACCTGTGGAAACATCAGA AGGATGTGTTTCTGAGCGCGGTGTCTACTGCGGCCAGTTCCCCTGGCAACAGGAATGGTGGCACACCTGC TCCGGGCAGCCCTGCAGAGAGTCTCAGAAAGAGCCACCCAGAGGTTATCAAGAAGTCTGTCCTGGACCAT ACCAGCTCTTTCTCCTTGGAGGAACTGCCCCCTCCTGTCCACCTGTCAAGGTCAGGGGAACACATGGATG TGTATGTGCCTGTGGCCTGTCACCCAGGCCATTTTGTCATCCAGCCCTGGCAGGAGATCCATAAGCTAGA AGTGCTGATGGAAGAGATGATCCTGTACTACAGCGTGTCCGAGGAGCGCCACATAGCGGTGGAGCGAGAC CAAGTGTACGCAGCCAAAGTGGAGAATAAGTGGTACAGGGTACTTTTAAAAGGAATCCTGACCAATGGGC TGGTGTCTGTGTACGAGCTGGACTACGGCAAGCATGAGCTCGTCAACATAAGGAAAGTGCAGCCCCTAGT GGACGTGTTCCGAAAGCTGCCCTTCCAGGCGGTCACCGCTCAACTGGCAGGCGTGAAGTGCAGCCAGTGG TCGGAGGAGGCTTCGATGGTGTTTCGAAATCACGTGGAGAAGAAAGCCCTGGTGGCGTTGGTGCAGACAG TTGTTGAGCACACTAATCCTTGGGACCGGAAAGTGGTGCTCTATCTCGTAGACACGTCGCTGCCCGACAC TGACACCTGGATTCATGACTTTATGTCACAGTATCTGCTAGAGCTTTCGAAAGTTAATTAATCACTGCTT GAGATGTTGACCGCTAATGAGATTGTTCTCAAAGCAATAATACAAAATGTGTAGGAGGGTCTTAAAACAT CTTATTTTTACTACATGGCTCTGACTGTTGTGGAGGATTGAAAAGAATATGCTTTGTTTGATGAAAGATA TTTAACAAGTTTTGTTTAATGCAGTTGACTTTTCAAAGAAAAATTGCACTTGAATTATTAATATAATATT AGAATAAAATGTTTAGCAATATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5367741 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5367741 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14663 same embryo
	EMAGE:14661 same embryo
	EMAGE:14666 same embryo
	EMAGE:14662 same embryo
	EMAGE:14665 same embryo
	EurExpress:euxassay_005556 same experiment
	MGI:4828654 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source