EMAGE mouse gene expression spatial database

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EMAGE:17284

Fstl1 follistatin-like 1 ( MGI:102793)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003685. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003685_01	euxassay_003685_02	euxassay_003685_03	euxassay_003685_04

euxassay_003685_05	euxassay_003685_06	euxassay_003685_07	euxassay_003685_08	euxassay_003685_09

euxassay_003685_10	euxassay_003685_11	euxassay_003685_12	euxassay_003685_13	euxassay_003685_14

euxassay_003685_15	euxassay_003685_16	euxassay_003685_17	euxassay_003685_18	euxassay_003685_19

euxassay_003685_20	euxassay_003685_21	euxassay_003685_22	euxassay_003685_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17284_wholemount_strong.wlz

17284_wholemount_moderate.wlz

17284_wholemount_weak.wlz

17284_wholemount_possible.wlz

17284_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17284_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 20 21 22 23

gland

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

alimentary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

integumental system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

cardiovascular system

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

urinary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

reproductive system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

respiratory system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

skeleton

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tail

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5703
Entity Detected:	Fstl1, follistatin-like 1 ( MGI:102793)
Sequence:	sense strand is shown >T5703 GATAAGCGACGCTCCCACCTTCGCCTCTAACTCGCTGCCGCCACCCTGCCCAGTGTCCTCCGGAGTCCCG GACCCGAGCACGATGTGGAAACGATGGCTGGCGCTCTCGCTGGTGACCATCGCCCTGGTCCACGGCGAGG AGGAACCTAGAAGCAAATCCAAGATCTGCGCCAATGTGTTTTGTGGAGCTGGCAGGGAATGTGCCGTCAC AGAGAAGGGGGAGCCCACGTGCCTCTGCATTGAGCAATGCAAACCTCACAAGAGGCCTGTGTGTGGCAGT AATGGCAAGACCTACCTCAACCACTGTGAACTTCATAGAGATGCCTGCCTCACTGGATCCAAGATCCAGG TTGATTATGATGGGCACTGCAAAGAAAAGAAGTCTGCGAGTCCATCTGCCAGCCCAGTTGTCTGCTATCA AGCTAACCGCGATGAGCTCCGACGGCGCCTCATCCAGTGGCTGGAAGCTGAGATCATTCCAGATGGCTGG TTCTCTAAAGGCAGTAACTACAGTGAGATCCTAGACAAGTACTTTAAGAGCTTTGATAATGGCGACTCTC ACCTGGACTCCAGTGAATTCCTGAAATTCGTGGAGCAGAATGAAACAGCCATCAACATCACCACTTATGC AGATCAGGAGAACAACAAACTGCTCAGAAGCCTCTGTGTTGACGCCCTCATTGAACTGTCTGATGAGAAC GCTGACTGGAAACTCAGCTTCCAAGAGTTCCTCAAGTGCCTCAACCCATCCTTCAACCCTCCTGAGAAGA AGTGTGCCCTGGAGGACGAAACCTATGCAGATGGAGCTGAGACTGAGGTGGACTGCAATCGCTGTGTCTG TTCCTGTGGCCACTGGGTCTGCACAGCAATGACCTGTGATGGAAAGAATCAGAAGGGGGTCCAGACCCAC ACAGAGGAGGAGAAGACAGGATATGTCCAGGAACTCCAGAAGCACCAGGGCACAGCAGAAAAGACCAAGA AGGTGAACACCAAAGAGATCTAAGAAGAGGCACAGAGCACCGTGTCCGGAGCCCAGCGCCTCCTCTTCAG CGCTGAGCCCAGTACACACAGAGTCTGCAGCAATCACCAAATCACTAGTATTTGCTTGTATGGCAGCGAA TCTTATTTTGTTTGTTTTGCAATAAAGGAAATGAGGGTGGCCAGCCTAGCGAGGGAAGGCCACAACCTTC ACCTGTAGGAATGCTTTAAGAGAAACTAAAGGACACCTTGGGACGAGAGGCAACTAAGGAAACAGCATCG GGTTGGCAGAGGAGCAGAGGCAGGTTTGAATGAAGCCTTTCTGGGGTCACAGCAGCTGCGAGGAGAATAC AGGAAAAGCATAGAGAAACATTGAACTAGCCCTGCTGGAGGAAGTGGGGGGAGCTTTGTAGGGAGGAACC CTGCTGCTTTGACCCTTGTCACCACTGTCAGCATGACAGACCTGCAGCAAGTCTGCTTCTCCTTTTGGTC CCAACAATCACCTGAACACACAGCCGCCCAACTAGTTACCTGTGTCCCTCAGCCTTGCATGGAGTTTCCT GGAGGAGGTGTTTAAATGATGCAGACACTTATGTACTTCAAGCGCCATGGAGACCTAACCAAATTTTTAA AATACATTTTTCTTTTTTTTTTTTTTTTGTTAACCAAAGGTGCTATTTCTCTGTAAAAGACTTTTTTCCA AGCTGACTTCATTCCTCAGTTATTACCGTTATATTATTGTTGTTTTTTAATATTTCATTTTTTGACTAGA TATTAAGCTTTTGTAATTATTTTTTCATTAGTCCTACTATTTCAGAAGTGAAGGTGAAGGGGGTTTGGGC ATTTTTCCAGGTACAGGGAACTCTGTAACACAAACAGCCCATACCCTGTCACATATTAGACCGGTTGCAG TTCGGAGCTGCACCCCAACCCAGAGCTTCTAGAAAATCAGCTCCATGCCACGAAGCACAAGAGGCCCCTC AGACAGAAGCCACAGGACAAAGCATCTTCATAGACAGCTGTTGAGATCCAAACAGTTAATTTGCTTTTGT TTCTTGTAAGAAGTTCCAAGGATGGACGCTCAGGCTATCCCAGCCTGCCAGCCTGCTGTGATCTGTGGCT AACTGGCAGAGTCAGCCACTGTGGTCCTTAGCTGCTCCTGTTTCTAGGTGTCAGTTTACTTAGTAAACTG GTAAGAATGAATCTTGGAATTTAATAAATGGTAGTTTGTGGTTTAGCCAACTGGTCCAGAGGGAGCTACC TTCTCCTTAGGATAGATGAATCTACACCATAAGAAAAACCAGCCAGGAATAGCATGGATGGGTTTTGCTT TGGTTGAAATGATCCTAGCAGGTGACTGGGTATGAGGACTTCATGGTCACTCTGCCCAGGAAGAGAGCGT GAAGGACAACTAGCAGCTTCCTTAGGGATGGTACACATGAGTGTGATCTCTGGAGTCAGAGGTTTCCCCA CACACATGATGATAAAACTTTTCAGATTTAGAGCGGTTAAAACTGGAGATCGAATCTGGATTGAGAATCA GCACTGGGGGAGAAACTGTTATTGAAAGTCAATCCTTTCTTTGAGCCTCCGAATAAACTATGGAGATTTT CCTGCATAGGAAAGTGTGGAATGTTGAGCTATTGAGATGGGAGTGGAATTCGTCCTAAATAGTTTTTCCT GGTCTCATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCCCTCCCTTTCAAAAGCTTTGAGCC TCGCTCATGCAGTCCAGATTTCATCCCCCTCTCCAAGTGCCTTGGAGAGCTCACAGCAGCAATGCCATCA TCAAAAGTTTTGCTGCTGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2647002 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647002 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17288 same embryo
	EMAGE:17283 same embryo
	EMAGE:17286 same embryo
	EMAGE:17285 same embryo
	EMAGE:17287 same embryo
	EurExpress:euxassay_003685 same experiment
	MGI:4824939 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source