EMAGE mouse gene expression spatial database

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EMAGE:17387

Rtn1 reticulon 1 ( MGI:1933947)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003752. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003752_01	euxassay_003752_02	euxassay_003752_03	euxassay_003752_04

euxassay_003752_05	euxassay_003752_06	euxassay_003752_07	euxassay_003752_08	euxassay_003752_09

euxassay_003752_10	euxassay_003752_11	euxassay_003752_12	euxassay_003752_13	euxassay_003752_14

euxassay_003752_15	euxassay_003752_16	euxassay_003752_17	euxassay_003752_18	euxassay_003752_19

euxassay_003752_20	euxassay_003752_21	euxassay_003752_22	euxassay_003752_23	euxassay_003752_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17387_wholemount_strong.wlz

17387_wholemount_moderate.wlz

17387_wholemount_weak.wlz

17387_wholemount_possible.wlz

17387_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17387_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 03 04 06 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 13 14 15

thoracic ganglion

strong

regional

strong expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 13 14 15 16 17

not examined

homogeneous

not examined expression: see section 01 02 03 04 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 04 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 15 16 17 18 19 moderate expression: see section 09

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09 weak expression: see section 02 03

genital tubercle of male

strong

regional

strong expression: see section 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7766
Entity Detected:	Rtn1, reticulon 1 ( MGI:1933947)
Sequence:	sense strand is shown >T7766 GGCGCGGAGACTCTGCAGCCTTCTGGAGTCCGGAGTCCCGGTAGGGACAGCAGCAGGGAGCGTCCGCGTC CCTAGCCAGCGCACCCCGGCCTTGCCAAGCCACAGTAGCCCAGCCTGGGGCACAGCCAGCCATGGCCGCA CCGCCGGATCTGCAAGATGAGCCGCTGTCTCTGGGTAGCCCCGGGTCCCAGTGGTTCGGGGGCCGGGGGG ATGGGGAAGACGAAGCGACGGCGGTGATGGGGGCCAGGCCAGCGCAGCAGGATGGGGAGCCCGCCTGGGG GTCGGGCGCTGGAGCTGGGGTAACGTCGTCGCGGGAACTGTGCTCCGGCCCCGCCCGGTCGCCTCCCGTC GCCATGGAAACTGCATCCACAGGTATGGCAGCTGTTCCCGATGCCCTCGACCACTCTCCCTCTTCAACAC TAAAAGATGGGGAAGGGGCATGTTACACATCCCTGATTTCTGACGTCTGCTATCCACCTCGGGAGGACTC TGCATACTTTACTGGAATTCTTCAGAAGGAAAATGGTCACATCACCACTTCCGAGAGCCCTGAGGAGCCG GAGACCCCCGGACCCTCGTTACCAGAAGTGCCCGGGATGGAGCCACAGGGCTTATTGAGTTCTGATTCTG GAATAGAGATGACTCCTGCTGAGTCCACTGAGGTGAACAAGATCCTAGCTGACCCCCTAGACCAGATGAA GGCAGAAGCCTACAAATACATTGACATAACCAGGCCACAGGAGGCAAAAGGCCAGGAGGAGCAACACCCT GGGCTAGAAGATAAAGACTTGGACTTTAAAGACAAGGACACTGAAGTCTCCACAAAAGCAGAAGGGGTTC GAGCACCAAACCAGCCAGCTCCTGTGGAGGGGAAACTCATCAAGGACCACTTATTCGAAGAATCTACCTT TGCTCCTTACATAGATGAGCTCTCTGATGAGCAGCACAGGGTGTCTCTGGTCACTGCCCCTGTCAAGATC ACACTGACGGAGATCGAACCTCCTCTTATGACTGCCACACAAGAGACAATCCCCGAGAAGCAAGACCTGT GTCTGAAGCCAAGTCCTGACACAGTGCCCACTGTCACTGTCTCAGAGCCTGAAGATGACAGCCCAGGATC TGTCACGCCTCCATCTTCTGGAACAGAACCATCTGCTGCAGAGTCCCAGGGGAAAGGCAGCGTCTCCGAG GATGAGTTGATTGCTGCCATTAAAGAAGCAAAGGGGTTATCATATGAAACCACCGAGAGTCCAAGGCCAG TGGGCCAGGTGGCCGACAAACCCAAAACCAAGACCAGGTCTGGGCTGCCCACCATCCCCAGTCCCCTCGA CCAGGAGGCCAGCAGTGCCGAGTCCGGAGACTCAGAAATTGAGCTGGTGTCAGAGGACCCCATGGCCTCT GAGGATGCCCTGCCCTCTGGCTATGTGAGCTTCGGTCATGTGTCGGGCCCGCCCCCCTCTCCTGCCTCTC CATCCATCCAGTACAGCATCCTGCGGGAGGAGCGTGAGGCCGAGCTGGACAGCGAGCTCATCATCGAGTC CTGTGATGCCTCCTCAGCCTCGGAGGAGAGCCCCAAGCGCGAGCAGGACTCGCCCCCGATGAAACCGGGT GCCCTGGACGCCATCCGCGAGGAGACAGGCTCTCGGGCCACCGAAGAACGAGCGCCCAGCCACCAAGGCC CTGTGGAGCCCGACCCGATGCTCAGCTTTGCCCCCGCTGCCGCCCTCCAATCCCGCCCAGAGCCATCGTC TGGAGATGGAGCCTCGGTCCCGGAGCCGCCCAGATCACAGCAGCAGAAGCCTGAAGAGGAAGCTGTGAGT TCCAGTCAAAGTCCCACGGCCACCGAAATTCCTGGACCACTTGGTTCTGGCCTCATGCCCCCTCTGCCTT TCTTCAACAAGCAAAAAGCTATTGACCTTCTGTACTGGCGGGACATCAAGCAGACTGGGATTGTGTTCGG GAGCTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCAGCGTTGTGAGCGTCGTTGCCTACCTGGCCCTG GCCGCCCTCTCTGCCACCATCAGCTTCCGCATCTACAAGTCCGTTCTACAAGCTGTGCAGAAAACAGATG AGGGTCACCCTTTCAAGGCCTACCTGGAGCTGGAGATCACCCTGTCCCAGGAGCAGATCCAGAAGTACAC AGACTGCCTGCAGCTGTATGTGAACAGCACTCTGAAGGAGCTACGGAGGCTTTTCCTGGTCCAGGACCTG GTGGATTCCTTAAAATTTGCAGTCCTCATGTGGCTCCTGACCTACGTTGGCGCGCTCTTCAATGGCCTGA CCCTGCTGCTTATGGCTGTGGTTTCGATGTTTACTCTACCTGTTGTGTACGTTAAGCACCAGGCACAAGT TGACCAATATCTGGGACTTGTGAGGACTCACATAAACACCGTCGTGGCAAAGATCCAGGCTAAAATCCCC GGCGCCAAGAGGCACGCTGAGTAGATGGTCACCCCAGGAGAAGCCGGACATGAGTGGGGGTGTCTGGAGT GGTAACAGCTCTCTCCTTCCTCGTTACTGCAAATTGATTGTTTCCCTTTCCTGGTACCATAATCTTAGAG GCAAACTTTGAGACAGGTGTTTTGAGGCTCTTCCTGTACTCTTAGGATATATGAGCCGCCCCCTCCCCAC CCCCGTGTCAAGCACTAAAGTATAGAGTGTGTTAGATGTGGTTTTTGATTTCGTGTTGCTAAAAAAAAAA AAAGAAAAGAAAAGAAAAAAAGTGCATGATGGTGAGAGCCCACGTTCTCCCTCCTCCCTCGATGTCCTCC CCTCTGCAACGCTTCTGTAGCTTCTACTGTCCCTGTGGCTAGGCCTTTCTTGCCAAGTGCGCTGACACAT AGTGGATCGCTTATCATGTCCTTGGGTTGCTGGATGGATTCATCTCTAATAACACTATATAGAATTGTAG GCCAATGTTTTAGCATTTCCCCACACACAGAGAACATAAAAATTAAAGTTAGTTCAACCATAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312477 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312477 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17385 same embryo
	EMAGE:17389 same embryo
	EMAGE:17388 same embryo
	EMAGE:17384 same embryo
	EMAGE:17386 same embryo
	EurExpress:euxassay_003752 same experiment
	MGI:4827828 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source