EMAGE mouse gene expression spatial database

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EMAGE:17503

Adam28 a disintegrin and metallopeptidase domain 28 ( MGI:105988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005826. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005826_01	euxassay_005826_02	euxassay_005826_03	euxassay_005826_04

euxassay_005826_05	euxassay_005826_06	euxassay_005826_07	euxassay_005826_08	euxassay_005826_09

euxassay_005826_10	euxassay_005826_11	euxassay_005826_12	euxassay_005826_13	euxassay_005826_14

euxassay_005826_15	euxassay_005826_16	euxassay_005826_17	euxassay_005826_18	euxassay_005826_19

euxassay_005826_20	euxassay_005826_21	euxassay_005826_22	euxassay_005826_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17503_wholemount_strong.wlz

17503_wholemount_moderate.wlz

17503_wholemount_weak.wlz

17503_wholemount_possible.wlz

17503_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17503_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 16

esophagus

moderate

regional

moderate expression: see section 11 12 13

trachea

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7747
Entity Detected:	Adam28, a disintegrin and metallopeptidase domain 28 ( MGI:105988)
Sequence:	sense strand is shown >T7747 GGCAGCCACTGCTCCTGGGATCGCAGAATGCAGCAATGGAGTCTTCTGGTAGTCTCTTTCCTTCTTTCTC CAGTTCCAGTAAGTGCAATAAAAGAACTCCCTAAAGCCAAGAAATATGAAGTGGTTTATCCCATAAGACT TCATCCATTGCGTAAAAGAGAGACCCAAGAGCCAGAGCCAAAGGAAACATTTGAAACTGAGCTAAGGTAC AAAATGACAGTAAATGGAAAGGTTGCTGTGCTGTATCTGAAGAAGAACAACAAGCTCCTTGCGCCTGACT ACTCGGAAACATACTATAATTCCAGTGGAAACAAGGTCACCACAAGCCCGCAAATCATGGATAGCTGTTA CTACCAAGGACACATCGTAAATGAGAAAGTTTCTGCAGCCAGCATCAGCACCTGTCAAGGACTACGGGGT TACATCAGTCAAGGAGATGAAAAGTATTTTATCGAACCTTTGAGCTCGGAGAACTTGGATGAACAGGCAC ATGCACTCTTCAAGGACGACTCCAATGAAGACCAGGAGAAGAGCAACTGTGGTGTGGATGATGCGCTATG GCTCCAAGGGCTGCATCAGGACGTGGCCCTCCCTGCCACCAGGTTGATTAAGTTGAATGATGGGATGGTT CAAGAACCTAAGAAATACATAGAATATTATGTGGTCCTGGATAATGGTGAGTTTAAGAAATACAATAAAA ATCTTGCTGAAATACGAAAGATAGTGCTTGAGATGGCCAATTACATCAACATGCTTTACAATAAGCTTGA TGCCCACGTGGCCTTAGTTGGAGTGGAAATCTGGACCGATGGGGATAAAATAAAGATAACACCAGATGCC AACACCACCCTGGAAAACTTCTCTAAGTGGAGGGGAAATGATCTGCTAAAACGAAAGCATCATGATATTG CCCAGCTAATCTCATCAACAGACTTTTCTGGATCAACAGTGGGTCTAGCCTTCATGTCGTCGATGTGTTC ACCTTACCATTCTGTTGGCATTGTTCAGGACCACAGTAACTACCATCTTCGAGTCGCAGGAACAATGGCT CATGAAATGGGTCACAATCTTGGCATGATTCATGACTACTTGAGCTGCAAGTGTCCATCTGAAGTCTGTG TAATGGAGCAGTCACTAAGGTTCCATATGCCTACAGACTTCAGCTCCTGCAGTCGTGTCAATTACAAACA GTTTCTTGAAGAAAAATTATCGCATTGCCTCTTTAATAGCCCATTGCCATCAGATATCATATCCACCCCA GTCTGTGGGAACCAGTTGTTGGAAATGAATGAGGACTGTGACTGTGGCACACCCAAGGAGTGTACTAACA AATGCTGTGATGCAAGGACCTGTAAAATTAAAGCAGGTTTCCAGTGTGCCCTGGGGGAATGCTGTGAGAA ATGCCAACTTAAAAAACCTGGGGTTGTGTGCAGAGCAGCAAAAGATGAGTGTGATCTGCCTGAAGTGTGT GATGGTAAATCCAGCCACTGCCCAGGTGACAGATTCAGAGTCAATGGCTCCCCTTGCCAAAATGGGCATG GTTACTGCTTGAAGGGCAAATGTCCCACCCTGCAGCAGCAGTGCATGGACATGTGGGGTCCAGGAACCAA GGTTGCAAATACATCATGTTACAAGCAGAATGAAGGTGGGACAAAGTACGGATACTGTCATGTGGAGAAT GGCACACACATGCCCTGCAAAGCAAAAGATGCCATGTGTGGGAAATTGTTCTGTGAAGGCGGATCAGGTG ATTTGCCCTGGAAAGGACTTACCATATCTTTCCTGACATGTAAATTATTTGATCCTGAAGACACAAGTCA AGGAGTAGACATGGTGGCCAATGGAACCAAGTGTGGAACTAACAAGGTGTGCATTAATGCTGAGTGTGTG GACATGGAGAAGACTTACAAGTCAGCCAACTGCTCCTCAAAGTGCAAGGGGCACGCAGTGTGTGACCATG AGCTTCAGTGTCAGTGCAAGGAAGGATGGGCCCCTCCTGACTGCGAGAATTCAGCCACAGTCTTCCACTT CTCCATCGTGGTTGGCGTGCTTTTCCCCCTAGCAGTCATATTTGTGGTGGTTGCTATAGTGATCCAGCGC CAAAGTGCCAGAAGGAAGCAGAGGAGAGTTCAGAGGCTACCATCCACCAAGGATGCCAAGCTACACAATC AGAAGTGTAGACCCCAAAAGGTGAAGGATGTTCAACCCCAGGAGATGAGTCAGATGAAAAAGCTCCATGT GTCTGATCTGCCCTCTGAAGAGCCGGAGCCTCCACCTGATGTCCTAATCACAAAGCCAAATTTCCCACCA CCACCAATTCCTGTTTCCTTGGACCCAAATGCAAAAGTCTGAAACAACAGTTAATCAGATGGTCAGAGTG GAAGCCTCGAAATTTTGGATGGAAAAAGAGATGTGCTTAGTTCTTTATTACTAGCGCTCTTAACCCTCAG GGGTTAGCATGTTCTGGTTTGTGCTGTATTTTGAGGAGATTAAAAGAAAGATACACTTTTGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310763 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310763 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17504 same embryo
	EMAGE:17502 same embryo
	EMAGE:17501 same embryo
	EMAGE:17505 same embryo
	EurExpress:euxassay_005826 same experiment
	MGI:4822977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source