EMAGE mouse gene expression spatial database

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EMAGE:18215

Pla2g7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) ( MGI:1351327)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011999. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011999_01	euxassay_011999_02	euxassay_011999_03	euxassay_011999_04

euxassay_011999_05	euxassay_011999_06	euxassay_011999_07	euxassay_011999_08	euxassay_011999_09

euxassay_011999_10	euxassay_011999_11	euxassay_011999_12	euxassay_011999_13	euxassay_011999_14

euxassay_011999_15	euxassay_011999_16	euxassay_011999_17	euxassay_011999_18	euxassay_011999_19

euxassay_011999_20	euxassay_011999_21	euxassay_011999_22	euxassay_011999_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18215_wholemount_strong.wlz

18215_wholemount_moderate.wlz

18215_wholemount_weak.wlz

18215_wholemount_possible.wlz

18215_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18215_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 12

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 17

cerebral cortex marginal layer

strong

regional

strong expression: see section 08 09 10 11 moderate expression: see section 04 05 06 07 12 14 15

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

pons ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 03 05 06 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16

spinal cord ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16

lens

strong

regional

strong expression: see section 01 02 weak expression: see section 23

neural retina

weak

regional

weak expression: see section 03 22

anterior naris epithelium

strong

regional

strong expression: see section 15

posterior naris epithelium

strong

regional

strong expression: see section 15

nasal cavity

strong

regional

strong expression: see section 11 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 14 16 17 18

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 15

nasal septum

strong

regional

strong expression: see section 13

stomach

moderate

regional

moderate expression: see section 04 05 06 07

rectum

moderate

regional

moderate expression: see section 13

midgut

moderate

regional

moderate expression: see section 13

renal cortex

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30084
Entity Detected:	Pla2g7, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) ( MGI:1351327)
Sequence:	sense strand is shown >T30084 AAGGTCGCCTCGACACTGTTTGGATCCCAAACAAAGAATATTTTTTGGGTCTTAGTATATTTCTTGGAAC ACCCAGTATTGTAGGCAATATTTTACACCTCTTATATGGTTCTCTGACAACTCCTGCAAGCTGGAATTCT CCTTTGAGGACTGGAGAAAAATACCCGCTCATTGTCTTTTCTCATGGTCTCGGAGCCTTCAGGACGATTT ATTCTGCTATTGGCATTGGCCTGGCATCTAATGGGTTTATAGTGGCCACTGTCGAACACAGAGACAGATC TGCATCGGCAACTTACTTTTTTGAAGACCAGGTGGCTGCAAAAGTGGAAAACAGGTCTTGGCTTTACCTG AGAAAAGTAAAACAAGAGGAGTCGGAAAGTGTCCGGAAAGAACAGGTTCAGCAAAGAGCAATAGAATGTT CCCGGGCTCTCAGTGCGATTCTTGACATTGAACATGGAGACCCAAAAGAGAATGTACTAGGTTCAGCTTT TGACATGAAACAGCTGAAGGATGCTATTGATGAGACTAAAATAGCTTTGATGGGACATTCTTTTGGAGGA GCAACAGTTCTTCAAGCCCTTAGTGAGGACCAGAGATTCAGATGTGGAGTTGCTCTTGATCCATGGATGT ATCCGGTGAACGAAGAGCTGTACTCCAGAACCCTCCAGCCTCTCCTCTTTATCAACTCTGCCAAATTCCA GACTCCAAAGGACATCGCAAAAATGAAAAAGTTCTACCAGCCTGACAAGGAAAGGAAAATGATTACAATC AAGGGCTCAGTGCACCAGAACTTTGACGACTTTACTTTTGTAACTGGCAAAATAATTGGAAACAAGCTGA CACTGAAAGGAGAAATCGATTCCAGAGTAGCCATCGACCTCACCAACAAAGCTTCGATGGCTTTCTTACA AAAGCATTTAGGGCTTCAGAAAGACTTTGATCAGTGGGACCCTCTGGTGGAAGGAGATGATGAGAACCTG ATTCCTGGGTCACCCTTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3707891), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 8999. Forward Primer - name:008999_F_IRAV20-23_I18_Pla2g7, sequence:AAGGTCGCCTCGACACTG; Reverse Primer - name:008999_R_SP6_IRAV20-23_I18_Pla2g7, sequence:TCAAAGGGTGACCCAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18217 same embryo
	EMAGE:18218 same embryo
	EMAGE:18220 same embryo
	EMAGE:18219 same embryo
	EMAGE:18216 same embryo
	EurExpress:euxassay_011999 same experiment
	MGI:4827244 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source