EMAGE mouse gene expression spatial database

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EMAGE:19382

Got1 glutamate oxaloacetate transaminase 1, soluble ( MGI:95791)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002196. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002196_01	euxassay_002196_02	euxassay_002196_03	euxassay_002196_04

euxassay_002196_05	euxassay_002196_06	euxassay_002196_07	euxassay_002196_08	euxassay_002196_09

euxassay_002196_10	euxassay_002196_11	euxassay_002196_12	euxassay_002196_13	euxassay_002196_14

euxassay_002196_15	euxassay_002196_16	euxassay_002196_17	euxassay_002196_18	euxassay_002196_19

euxassay_002196_20	euxassay_002196_21	euxassay_002196_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19382_wholemount_strong.wlz

19382_wholemount_moderate.wlz

19382_wholemount_weak.wlz

19382_wholemount_possible.wlz

19382_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19382_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 15 16 17 weak expression: see section 18 19 20

vertebral axis musculature

moderate

regional

moderate expression: see section 02 04 05 06 07 08 12 13 14 15 16 17 18 19 20 21 22

adrenal gland

strong

homogeneous

strong expression: see section 05 06 12 13 14

thymus primordium

moderate

homogeneous

moderate expression: see section 07 08 09 10 11

brain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 01 02 03 04

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 05 13

spinal cord

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 weak expression: see section 04

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 11 12 13

not examined

regional

not examined expression: see section 02 03 21 22

neural retina

moderate

regional

moderate expression: see section 02 03 21 22 weak expression: see section 01 04

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 14 15 16 17

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

oral epithelium

moderate

regional

moderate expression: see section 01 10 11 12 13 14 15

renal cortex

moderate

regional

moderate expression: see section 13 weak expression: see section 05 06 07 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6040
Entity Detected:	Got1, glutamate oxaloacetate transaminase 1, soluble ( MGI:95791)
Sequence:	sense strand is shown >T6040 CCCGGCTCGTTCTCGAGGTCTCGGCACATTCTGTCGCGATGGCGCCTCCATCAGTCTTTGCCCAGGTTCC GCAAGCTCCTCCGGTTCTGGTCTTTAAGCTCACTGCGGACTTCCGGGATGATCCAGATCCCCGCAAGGTT AACCTCGGCGTGGGAGCGTACCGCACAGATGAATCTCAGCCCTGGGTTTTGCCAGTAGTGAGGAAGGTCG AACAGAAGATTGCTAATGACAACAGCCTCAACCACGAGTACCTGCCCATCCTGGGCCTGGCAGAGTTCCG GAGCTGTGCTTCTCGCCTAGTTCTTGGGGACAACAGCCCGGCTATCAGGGAGAATCGGGTTGGAGGGGTG CAGTCTTTGGGAGGGACAGGCGCTCTTCGGATTGGAGCTGACTTCTTAGGGCGATGGTACAATGGTACAG ATAACAAGAACACACCAATCTACGTATCATCGCCAACCTGGGAGAACCATAATGCTGTGTTTTCTGCCGC CGGTTTTAAGGACATTCGGCCCTATTGCTACTGGGATGCGGAGAAGAGAGGACTGGACCTCCAGGGTTTC CTGAATGATCTGGAGAATGCCCCCGAGTTCTCCATCTTTGTCCTCCATGCCTGTGCGCACAACCCAACAG GGACCGACCCGACTCCAGAGCAGTGGAAGCAGATCGCTGCTGTCATGCAGCGCCGTTTTCTGTTCCCCTT CTTTGACTCAGCCTATCAGGGCTTTGCATCTGGAGACCTAGAGAAAGATGCGTGGGCTATTCGCTATTTT GTGTCTGAAGGCTTCGAGCTCTTCTGTGCCCAGTCCTTCTCCAAGAACTTCGGGCTCTACAATGAGAGAG TGGGGAATCTGACCGTGGTCGGAAAAGAGTCTGACAGCGTCCTGCGGGTCCTTTCCCAGATGGAGAAGAT TGTACGAATCACCTGGTCCAATCCCCCAGCCCAAGGAGCTCGGATTGTGGCCGCCACCCTCTCTGACCCG GAGCTCTTTAAGGAGTGGAAAGGTAACGTGAAGACAATGGCTGACCGGATTCTGACCATGAGATCCGAAC TCAGGGCAAGACTAGAAGCTCTCAAGACCCCCGGGACTTGGTCTCACATCACTGAGCAGATTGGAATGTT CAGTTTCACCGGCTTGAACCCCAAGCAGGTCGAGTATTTGGTCAACGAGAAGCATATCTATCTCCTGCCG AGTGGTCGGATCAACATGTGCGGCTTGACCACCAAGAACCTAGATTACGTCGCTACCTCCATCCATGAAG CCGTCACCAAAATCCAGTGAAGAAACACCTCGTAGTTCACGCCACCAAAGCAGTTCTTGTCACACCTTTC CTGCCTGCGCAAACCTAGCTGTACCTAGCTGTACACGTCCTTTATTAGAGATGACCGAGGGGAAGCAGCT GCTGTTTAGCTGACCCCCCACATGAGAAGACGTTTCTTGAAATGAGCCCCGGGGGCTCTGGGGTTAGAGC CTGTTGGAAACCAGAGTAGATTAAAGTTATTTAAGAATAAAAAGAACTTTGGCTATGAGATGTAATCACC TTGCCTTCCTCTCTAGCATTCTGCAGGAGTGATGCCCACGGAGCCTTGGGCTTCTGCGTGTTGCCTGACT CTGTACAAAAGTCTAGTCTCAAAGATCAAGTTGGTCTGAGGAGCCGGATGTGACTGTGGGTGTTGGCTGG GGCATTAAAACTCATCATCGCCACCCATCTCTGTCTCTCGTCACCCTGATCTCCCCGCACGGTTGTGTCC CTGGTCTGGAACATTAGTTTTTTTAAGGCAACTGTGGCCAATATTTATATCATGACATACAAATGGATTT ACGTATTTGACTGAAATGAAAGTTCCACTAAACGGTATTTGCTCTTGTGATATGTGGCACATTGTGATAT TTTCTTAGTCTGTTCTGTTTCATTTAAAAAATAAAACTGCTGATCAAGACAAGTGAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3489628 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3489628 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19381 same embryo
	EMAGE:19379 same embryo
	EMAGE:19378 same embryo
	EMAGE:19380 same embryo
	EMAGE:19377 same embryo
	EurExpress:euxassay_002196 same experiment
	MGI:4825150 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source