EMAGE mouse gene expression spatial database

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EMAGE:19477

Cdca3 cell division cycle associated 3 ( MGI:1315198)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004852. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004852_01	euxassay_004852_02	euxassay_004852_03	euxassay_004852_04

euxassay_004852_05	euxassay_004852_06	euxassay_004852_07	euxassay_004852_08	euxassay_004852_09

euxassay_004852_10	euxassay_004852_11	euxassay_004852_12	euxassay_004852_13	euxassay_004852_14

euxassay_004852_15	euxassay_004852_16	euxassay_004852_17	euxassay_004852_18	euxassay_004852_19

euxassay_004852_20	euxassay_004852_21	euxassay_004852_22	euxassay_004852_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19477_wholemount_strong.wlz

19477_wholemount_moderate.wlz

19477_wholemount_weak.wlz

19477_wholemount_possible.wlz

19477_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19477_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

hypothalamus ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10 11 12

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 07 08 09 12 13 14

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 15 16 17 18 19 20

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 11 15 16 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

ventral grey horn

moderate

single cell

moderate expression: see section 11 12 13 14 15 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 12 13 14 15 16

liver left lobe

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

liver right lobe

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23

renal cortex

moderate

homogeneous

moderate expression: see section 08 09 10 11 17 18 19 20

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6550
Entity Detected:	Cdca3, cell division cycle associated 3 ( MGI:1315198)
Sequence:	sense strand is shown >T6550 CCCACGCGTCCGCCCACGCGTCCGGTTCGGCCAGAGGTCGAGCTGGGGCCCGCCGGGCCCCGTCCCCAGC CCCAGTGGAGATGGGTTCGACTCAGAGCGTCTCAGGCACTCCAGCACGGCCTCTGCCACGCAACAAGCAA GTGGCTCGAGTAGCAGACCCTCGTTCACCTAGTGCTGGCATCCAGCGCACTCCTATTCAGGTAGAGAGCT CTCCACAGCCAAGCCTACCAGCAGAACAGCTGAACGGTCTCAAACAGGCACAAGACCCAGATCCCCGCTC TCCTACTCTTGGCATTGCACGGACACCCATGAAGATCAGTGGTCCAGACCCTCAGTGCTCACTGGTGAAA GAGCTGAGCGAAGTATTGGAGACGGAAGCGTCGGAATCGATTTCCTCCCCAGAGCTTGCTCTGCCCCGGG AAACGCCTTTATTTTCTGACCTGGACCTGTCTTCAGGTCCTCAGTTATCCCCTGAGGACCAGTTACTGCC TTGGAGCCAGGCTGAACTCGATCCCAAACAGGTGTTTACCAAGGAGGAAGCCAAACAATCCGCAGAAACT ATAGCTGCCAGCCAGAACTCAGACAAGCCCTCCAGAGACCCAGAGACTCCCCAGTCCTCAGGTTCTAAGC GCAGCAGACGAAAAGCAAACAGCAAGGTTCTAGGGAGGTCCCCTCTCACCATCCTGCAGGATGACAACTC CCCTGGGACCTTGACACTACGACAGGGTAAGCGGCCTTCTGCCCTCAGTGAGAACGTTAAGGACCTAAAG GAAGGAGTCGTTCTTGGAACTGGAAGATTTCTCAAAGCTGGAGGAGGAGCACGGGAGCCAAACCAGGACC ACGACAAGGAAAATCAGCATTTTGCCTTGTTGGAGAGCTAGGGCTGTGCTGGAGTCATCCCCAAGGGCAC CAAAGAAAGCCCTGTTCTGATTCCTCTCAGGCACCAACTCTGGTACTTGGACATCGTTCTTTTAAAATGT TTTATGTGTTTCCTGTATATTAAAGTAGATGATTTTAAATGATGCTTTAAGAAGTTAGAGCAAAGCTACA CCGGGTCTTGTGTGTATAGAATGGCATCCTAGGGGCCTGTGAGACCTGGATAGATCTCTCTCCTGCCACT TCTGAGCCTCCTTTATGTAGCTTAAGCTTTGATGTCCCCAGAGCTCTGCCTTTGGCCCTCTTCACTTCTT GTTTGGGTTTAGCTATTGCTTACCTGCTTGTAATTTCCAGGTCCCCCTCTCGACATAAACACTCAACGTT AGTTCCCCTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3592108 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592108 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19478 same embryo
	EMAGE:19479 same embryo
	EMAGE:19475 same embryo
	EMAGE:19480 same embryo
	EMAGE:19476 same embryo
	EurExpress:euxassay_004852 same experiment
	MGI:4823758 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source